Viscoelastic substrate decouples cellular traction force from other related phenotypes

Dwivedi, Nehal; Das, Shambaditya; Bellare, Jayesh; Majumder, Abhijit

doi:10.1016/j.bbrc.2021.01.027

Cited by 6 publications

(3 citation statements)

References 39 publications

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“…As time elapsed, these forces were gradually decreased due to the counteraction effects caused by various dissipative events such as the unbinding of weak bonds, polymer disentanglement, protein unfolding, and molecule slipping within the hydrogel. 238 , 239 For instance, alginate has been extensively used as the skeleton material to tune the viscoelasticity (e.g., stress relaxation speed or plasticity) of as-prepared hydrogels via varying its MW. The finely regulation of viscoelasticity is convenient for exclusively studying the effect of hydrogel stress relaxation on cell behavior because cells could not degrade alginate-based hydrogels.…”

Section: Influences Of Hydrogel Properties On Cell Behavior and Signaling Pathwaysmentioning

confidence: 99%

Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity

Cao

Duan

Yan

et al. 2021

Sig Transduct Target Ther

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256

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Hydrogel is a type of versatile platform with various biomedical applications after rational structure and functional design that leverages on material engineering to modulate its physicochemical properties (e.g., stiffness, pore size, viscoelasticity, microarchitecture, degradability, ligand presentation, stimulus-responsive properties, etc.) and influence cell signaling cascades and fate. In the past few decades, a plethora of pioneering studies have been implemented to explore the cell–hydrogel matrix interactions and figure out the underlying mechanisms, paving the way to the lab-to-clinic translation of hydrogel-based therapies. In this review, we first introduced the physicochemical properties of hydrogels and their fabrication approaches concisely. Subsequently, the comprehensive description and deep discussion were elucidated, wherein the influences of different hydrogels properties on cell behaviors and cellular signaling events were highlighted. These behaviors or events included integrin clustering, focal adhesion (FA) complex accumulation and activation, cytoskeleton rearrangement, protein cyto-nuclei shuttling and activation (e.g., Yes-associated protein (YAP), catenin, etc.), cellular compartment reorganization, gene expression, and further cell biology modulation (e.g., spreading, migration, proliferation, lineage commitment, etc.). Based on them, current in vitro and in vivo hydrogel applications that mainly covered diseases models, various cell delivery protocols for tissue regeneration and disease therapy, smart drug carrier, bioimaging, biosensor, and conductive wearable/implantable biodevices, etc. were further summarized and discussed. More significantly, the clinical translation potential and trials of hydrogels were presented, accompanied with which the remaining challenges and future perspectives in this field were emphasized. Collectively, the comprehensive and deep insights in this review will shed light on the design principles of new biomedical hydrogels to understand and modulate cellular processes, which are available for providing significant indications for future hydrogel design and serving for a broad range of biomedical applications.

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Section: Influences Of Hydrogel Properties On Cell Behavior and Signaling Pathwaysmentioning

confidence: 99%

Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity

Cao

Duan

Yan

et al. 2021

Sig Transduct Target Ther

404

256

View full text Add to dashboard Cite

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“…[73][74][75]80 Charrier et al observed that cells present smaller focal adhesion on viscoelastic substrates as compared to elastic gels, which is in agreement with the hypothesis that low traction forces develop at the cell substrate interface due to the relaxation of the forces exerted by the cell on the substrate, 73 and that cells apply less force on viscoelastic substrates. 81 Consequently, the viscous dissipation of the cell-generated forces into the matrix would prevent cell spreading. 73,80 However, this effect might be stiffness dependent.…”

Section: Biomaterials Science Papermentioning

confidence: 99%

Interplay of matrix stiffness and stress relaxation in directing osteogenic differentiation of mesenchymal stem cells

et al. 2022

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“…The studies on the influence of viscoelasticity on cell properties conducted during the last several decades initially focused on cell spreading [10,11] and then gradually shifted to cellular functions and collective behaviors. [12][13][14][15][16] This work motivated us to examine the consequent changes in the activities of macrophages modified by the viscoelastic properties of ECMs.…”

Section: Introductionmentioning

confidence: 99%

Substrate Viscoelasticity Amplifies Distinctions between Transient and Persistent LPS‐Induced Signals

Zhou

2021

Adv Healthcare Materials

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Macrophages settle in heterogeneous microenvironments rendered by other cells and extracellular matrices. It is well known that chemical stimuli direct macrophage behavior; however, the contributions of viscosity, which increases in inflammatory tissues but not in tumors, are ignored in immune responses including effective activation and timely attenuation. This paper demonstrates that transient lipopolysaccharide (LPS)-treated macrophages benefit from elastic substrates, whereas viscoelastic substrates with similar storage moduli support the inflammatory responses of macrophages under persistent stimulations and consequently amplify the distinctions between the transient and persistent LPS-induced transcriptional programs. Actin filaments (F-actin) fluctuate in line with transcriptional profiles and can be mathematically predicted by a clutch-like model. Moreover, viscosity modifies immune responses through transcription factors NF-𝜿B and C/EBP𝜹, which act as switches discriminating transient and persistent infections. Interestingly, enhanced immune responses, consistent with the lower activated states, are attenuated promptly by the actin nucleation-related translocation of ATF3 to nuclei. These findings suggest that the substrate viscoelasticity induces more intense inflammation only in the case of persistent infection and promotes more sensitively perceiving the duration of infection through the F-actin correlated transcription factors. In addition, it may facilitate the cognition of immune response in inflammatory and cancerous microenvironments and have a wide range of applications in inflammatory regulations.

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Viscoelastic substrate decouples cellular traction force from other related phenotypes

Cited by 6 publications

References 39 publications

Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity

Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity

Interplay of matrix stiffness and stress relaxation in directing osteogenic differentiation of mesenchymal stem cells

Substrate Viscoelasticity Amplifies Distinctions between Transient and Persistent LPS‐Induced Signals

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