Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications

Vargas, Maria Isabel Patiño; Martínez-García, Francisco Drusso; Offens, Freya; Becerra, Natalia; Múnera, Luz Marina Restrepo; Mei, Henny C. van der; Harmsen, Martin C.; Kooten, Theo G. van; Sharma, Prashant K.

doi:10.1016/j.bioadv.2022.212967

Cited by 12 publications

(7 citation statements)

References 49 publications

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“…They found there were changes in cell metabolism dependent on the displacement of Maxwell elements, and that fibroblast proliferation could be controlled based on agarose concentration. 54 These results indicate that viscoelastic properties of hydrogels can be used as a material-based modulator for cell metabolism and proliferation. A different study fabricated an electrospun multilayer nanofibrous scaffold composed of silk fibroin (SF) blended with PVA, silk sericin loaded with silver(I) sulfadiazine, and SF blended with PCL.…”

Section: Other Methodsmentioning

confidence: 88%

“…Another group studied the effect of viscoelastic properties of plasma‐agarose composite hydrogels on cell behavior. They found there were changes in cell metabolism dependent on the displacement of Maxwell elements, and that fibroblast proliferation could be controlled based on agarose concentration 54 . These results indicate that viscoelastic properties of hydrogels can be used as a material‐based modulator for cell metabolism and proliferation.…”

Section: Skin/wound Healingmentioning

confidence: 95%

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Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Adhikari

Stager

Krebs

2023

J Biomedical Materials Res

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The field of biomaterials aims to improve regenerative outcomes or scientific understanding for a wide range of tissue types and ailments. Biomaterials can be fabricated from natural or synthetic sources and display a plethora of mechanical, electrical, and geometrical properties dependent on their desired application. To date, most biomaterial systems designed for eventual translation to the clinic rely on soluble signaling moieties, such as growth factors, to elicit a specific cellular response. However, these soluble factors are often limited by high cost, convoluted synthesis, low stability, and difficulty in regulation, making the translation of these biomaterials systems to clinical or commercial applications a long and arduous process. In response to this, significant effort has been dedicated to researching cell‐directive biomaterials which can signal for specific cell behavior in the absence of soluble factors. Cells of all tissue types have been shown to be innately in tune with their microenvironment, which is a biological phenomenon that can be exploited by researchers to design materials that direct cell behavior based on their intrinsic characteristics. This review will focus on recent developments in biomaterials that direct cell behavior using biomaterial properties such as charge, peptide presentation, and micro‐ or nano‐geometry. These next generation biomaterials could offer significant strides in the development of clinically relevant medical devices which improve our understanding of the cellular microenvironment and enhance patient care in a variety of ailments.

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Section: Other Methodsmentioning

confidence: 88%

Section: Skin/wound Healingmentioning

confidence: 95%

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Adhikari

Stager

Krebs

2023

J Biomedical Materials Res

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“…SEM microphotographs of hydrogels are employed to determine pore size, pore distribution, and porosity percentage, as well as fiber thickness and fiber orientation [ 17 , 38 , 63 , 72 , 73 , 74 ]. In cell-loaded hydrogels, the visualization of the cells is also possible [ 80 , 81 , 82 , 83 , 84 ]. The analytical capabilities of SEM include X-ray-based tools, such as energy dispersive X-Ray spectroscopy (EDX).…”

Section: Electron-based Techniquesmentioning

confidence: 99%

A Beginner’s Guide to the Characterization of Hydrogel Microarchitecture for Cellular Applications

Martínez-García

Fischer

Hayn

et al. 2022

Gels

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The extracellular matrix (ECM) is a three-dimensional, acellular scaffold of living tissues. Incorporating the ECM into cell culture models is a goal of cell biology studies and requires biocompatible materials that can mimic the ECM. Among such materials are hydrogels: polymeric networks that derive most of their mass from water. With the tuning of their properties, these polymer networks can resemble living tissues. The microarchitectural properties of hydrogels, such as porosity, pore size, fiber length, and surface topology can determine cell plasticity. The adequate characterization of these parameters requires reliable and reproducible methods. However, most methods were historically standardized using other biological specimens, such as 2D cell cultures, biopsies, or even animal models. Therefore, their translation comes with technical limitations when applied to hydrogel-based cell culture systems. In our current work, we have reviewed the most common techniques employed in the characterization of hydrogel microarchitectures. Our review provides a concise description of the underlying principles of each method and summarizes the collective data obtained from cell-free and cell-loaded hydrogels. The advantages and limitations of each technique are discussed, and comparisons are made. The information presented in our current work will be of interest to researchers who employ hydrogels as platforms for cell culture, 3D bioprinting, and other fields within hydrogel-based research.

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“…Whole-cell immobilization of different cell types ranging from heterotrophic microbes and animal cells to photosynthetic organisms has been extensively studied for a variety of applications including tissue engineering, , 3D cell culture and analysis matrices, − wastewater purification, − and biotransformation or production of chemicals. − In these biohybrid platforms, the properties of both the cells and the matrix are pivotal factors affecting the operational performance of the system as a whole. Cell-laden hydrogels are most often used in the biomedical field and have generally been characterized using imaging techniques that can preserve the sample ultrastructure (e.g., scanning electron microscopy (SEM), confocal laser scanning microscopy, and atomic force microscopy (AFM)), mechanical assessments (compressive and tensile testing, rheology, swelling behavior, porosity, and gel degradation), , and by evaluating the biological compatibility of the system (cell viability, growth, and morphology). ,, The selection of methods is heavily dependent on the end application, which defines the operational framework and boundary conditions for the applied materials.…”

Section: Introductionmentioning

confidence: 99%

“…Cell-laden hydrogels are most often used in the biomedical field and have generally been characterized using imaging techniques that can preserve the sample ultrastructure (e.g., scanning electron microscopy (SEM), confocal laser scanning microscopy, and atomic force microscopy (AFM)), 13 mechanical assessments (compressive and tensile testing, rheology, swelling behavior, porosity, and gel degradation), 14 , 15 and by evaluating the biological compatibility of the system (cell viability, growth, and morphology). 10 , 11 , 15 The selection of methods is heavily dependent on the end application, which defines the operational framework and boundary conditions for the applied materials.…”

Section: Introductionmentioning

confidence: 99%

Mapping Nanocellulose- and Alginate-Based Photosynthetic Cell Factory Scaffolds: Interlinking Porosity, Wet Strength, and Gas Exchange

Levä,

Rissanen,

Nikkanen

et al. 2023

Biomacromolecules

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To develop efficient solid-state photosynthetic cell factories for sustainable chemical production, we present an interdisciplinary experimental toolbox to investigate and interlink the structure, operative stability, and gas transfer properties of alginate- and nanocellulose-based hydrogel matrices with entrapped wild-type Synechocystis PCC 6803 cyanobacteria. We created a rheological map based on the mechanical performance of the hydrogel matrices. The results highlighted the importance of Ca2+-cross-linking and showed that nanocellulose matrices possess higher yield properties, and alginate matrices possess higher rest properties. We observed higher porosity for nanocellulose-based matrices in a water-swollen state via calorimetric thermoporosimetry and scanning electron microscopy imaging. Finally, by pioneering a gas flux analysis via membrane-inlet mass spectrometry for entrapped cells, we observed that the porosity and rigidity of the matrices are connected to their gas exchange rates over time. Overall, these findings link the dynamic properties of the life-sustaining matrix to the performance of the immobilized cells in tailored solid-state photosynthetic cell factories.

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Viscoelastic properties of plasma-agarose hydrogels dictate favorable fibroblast responses for skin tissue engineering applications

Cited by 12 publications

References 49 publications

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

A Beginner’s Guide to the Characterization of Hydrogel Microarchitecture for Cellular Applications

Mapping Nanocellulose- and Alginate-Based Photosynthetic Cell Factory Scaffolds: Interlinking Porosity, Wet Strength, and Gas Exchange

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