Viscoelastic and Histologic Properties in Scarred Rabbit Vocal Folds After Mesenchymal Stem Cell Injection

Abstract

The treated scarred vocal folds showed persistent MSC. Injection of scarred rabbit vocal folds with MSC rendered improved viscoelastic parameters and less signs of scarring expressed as collagen content in comparison to the untreated scarred vocal folds.

In Vivo Comparison of Biomimetic Approaches for Tissue Regeneration of the Scarred Vocal Fold

“…Prophylaxis, which has been shown to enhance wound repair and minimize scar formation after injury, has already been identified and has been a focus of the vocal fold scarring literature. 3,[10][11][12]25 It would be expected that the two approaches for treating vocal fold scarring-early treatment versus late treatment-would have mechanistically different biological responses. The cellular response would be phenotypically different for tissue that has been recently injured versus a chronic injury.…”

“…Cell approaches have included autologous and nonautologous mesenchymal stem cells, [1][2][3] autologous fibroblasts, 4 and human embryonic stem cells. 5 Various engineered scaffolds tested include acellular xenogeneic scaffolds, 6,7 collagen hydrogels, 8 and hyaluronic acid (HA)-based hydrogels.…”

Bioengineering the Vocal Fold: A Review of Mesenchymal Stem Cell Applications

“…Improved viscoelastic measures have been reported for vocal folds treated with BM MSCs alone in a rabbit model (Hertegård et al, 2006a;Svensson et al, 2010). Improved tissue morphology (less granulation tissue, less rough vocal fold edge) has been reported in injured vocal folds treated with BM MSCs as compared with controls, over a recovery periods lasting from four and twelve weeks (Hertegård et al, 2006a;Kanemaru et al, 2005, Svensson et al, 2010. Figure 1 demonstrates the resolution of one dog's granuloma and rough vocal fold morphology over a 24 week recovery period.…”

“…The literature to date has focused largely on the expression and some quantification of the ECM components known to be involved with wound healing, such as collagen, fibronectin, and hyaluronic acid. A reduction in collagen I content (Hertegård et al, 2006a, Svensson et al, 2010 and an increase in TGF-β1 production have been reported in vocal folds injected with BM MSCs alone versus scarred, untreated controls. Additionally, it has been found in vitro that a coculture of ASCs and scar fibroblasts (SF) had decreased collagen production levels and SF proliferation rates when compared with SFs in monoculture (Kumai et al, 2009).…”

Histological analysis of autologous fascia graft implantation into the rabbit voice muscle

“…duprat 14 used laryngofissure to expose both vocal folds in rabbits. Other authors, such as Hertegard et al 15 , used zero degree optics for children. Campognolo et al 16 proposed using a laryngoscope based on killian's nasal speculum.…”