Visceral white adipose tissue and serum proteomic alternations in metabolically healthy obese patients undergoing bariatric surgery

Doulamis, Ilias P.; Konstantopoulos, Panagiotis; Tzani, Aspasia; Antoranz, Asier; Minia, Angeliki; Daskalopoulou, A.; Charalampopoulos, Anestis; Alexopoulos, Leonidas G.; Perrea, D.; Menenakos, Evangelos

doi:10.1016/j.cyto.2018.11.017

Cited by 18 publications

(13 citation statements)

References 46 publications

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“…This also corroborates the findings from another study which found that level of CSF-1 correlated with the number of omental macrophages which in turn were associated with waist circumference 47 . Moreover, serum protein level of CCL-3 has been found to correlate with visceral white adipose tissue in healthy obese individuals undergoing bariatric surgery 48 . Thus, results suggest a low-grade inflammatory state in VEGFA, CSF-1 and CCL3 which is related to obesity and BMI.…”

Section: Discussionmentioning

confidence: 98%

Longitudinal plasma inflammatory proteome profiling during pregnancy in the Born into Life study

Hedman

Lundholm

Andolf

et al. 2020

Sci Rep

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The maternal immune system is going through considerable changes during pregnancy. However, little is known about the determinants of the inflammatory proteome and its relation to pregnancy stages. Our aim was to investigate the plasma inflammatory proteome before, during and after pregnancy. In addition we wanted to test whether maternal and child outcomes were associated with the proteome. A cohort of 94 healthy women, enrolled in a longitudinal study with assessments at up to five time points around pregnancy, ninety-two inflammatory proteins were analysed in plasma with a multiplex Proximity Extension Assay. First, principal components analysis were applied and thereafter regression modelling while correcting for multiple testing. We found profound shifts in the overall inflammatory proteome associated with pregnancy stage after multiple testing (p < .001). Moreover, maternal body mass index (BMI) was associated with inflammatory proteome primarily driven by VEGFA, CCL3 and CSF-1 (p < .05). The levels of most inflammatory proteins changed substantially during pregnancy and some of these were related to biological processes such as regulation of immune response. Maternal BMI was significantly associated with higher levels of three inflammation proteins calling for more research in the interplay between pregnancy, inflammation and BMI.

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Section: Discussionmentioning

confidence: 98%

Longitudinal plasma inflammatory proteome profiling during pregnancy in the Born into Life study

Hedman

Lundholm

Andolf

et al. 2020

Sci Rep

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“…Consistently, high resistin levels also correlate with increased risk of all-cause and cardiovascular death [80]. It is also remarkable that the excess of resistin is among the cytokines that define the metabolic unhealthy state, associated with obesity, as demonstrated by the decrease of circulating resistin levels after bariatric surgery [81,82].…”

Section: Specific Adipokinesmentioning

confidence: 99%

Relevance of Leptin and Other Adipokines in Obesity-Associated Cardiovascular Risk

et al. 2019

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Obesity, which is a worldwide epidemic, confers increased risk for multiple serious conditions including type 2 diabetes, nonalcoholic fatty liver disease, and cardiovascular diseases. Adipose tissue is considered one of the largest endocrine organs in the body as well as an active tissue for cellular reactions and metabolic homeostasis rather than an inert tissue only for energy storage. The functional pleiotropism of adipose tissue relies on its ability to synthesize and release a large number of hormones, cytokines, extracellular matrix proteins, and growth and vasoactive factors, which are collectively called adipokines known to influence a variety of physiological and pathophysiological processes. In the obese state, excessive visceral fat accumulation causes adipose tissue dysfunctionality that strongly contributes to the onset of obesity-related comorbidities. The mechanisms underlying adipose tissue dysfunction include adipocyte hypertrophy and hyperplasia, increased inflammation, impaired extracellular matrix remodeling, and fibrosis together with an altered secretion of adipokines. This review describes the relevance of specific adipokines in the obesity-associated cardiovascular disease.

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“…Human soluble TGFa (sTGFa), soluble HB-EGF (sHB-EGF), and sAREG were measured in HPTC culture media samples and sAREG was measured in human serum samples using microsphere-based Luminex Technology and ELISA kits (DY239, DY259B, and DY262, respectively; all from R&D Systems) as previously described. 7,17 Statistical Analyses All results are reported as the mean6SD. Comparison of two groups was performed using an unpaired, two-tailed t test or a Pearson correlation analysis where appropriate.…”

Section: Elisamentioning

confidence: 99%

Proximal Tubule–Derived Amphiregulin Amplifies and Integrates Profibrotic EGF Receptor Signals in Kidney Fibrosis

Kefaloyianni

Raja

Schumacher

et al. 2019

JASN

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BackgroundSustained activation of EGF receptor (EGFR) in proximal tubule cells is a hallmark of progressive kidney fibrosis after AKI and in CKD. However, the molecular mechanisms and particular EGFR ligands involved are unknown.MethodsWe studied EGFR activation in proximal tubule cells and primary tubular cells isolated from injured kidneys in vitro. To determine in vivo the role of amphiregulin, a low-affinity EGFR ligand that is highly upregulated with injury, we used ischemia-reperfusion injury or unilateral ureteral obstruction in mice with proximal tubule cell–specific knockout of amphiregulin. We also injected soluble amphiregulin into knockout mice with proximal tubule cell–specific deletion of amphiregulin’s releasing enzyme, the transmembrane cell-surface metalloprotease, a disintegrin and metalloprotease-17 (ADAM17), and into ADAM17 hypomorphic mice.ResultsYes-associated protein 1 (YAP1)–dependent upregulation of amphiregulin transcript and protein amplifies amphiregulin signaling in a positive feedback loop. YAP1 also integrates signals of other moderately injury-upregulated, low-affinity EGFR ligands (epiregulin, epigen, TGFα), which also require soluble amphiregulin and YAP1 to induce sustained EGFR activation in proximal tubule cells in vitro. In vivo, soluble amphiregulin injection sufficed to reverse protection from fibrosis after ischemia-reperfusion injury in ADAM17 hypomorphic mice; injected soluble amphiregulin also reversed the corresponding protective proximal tubule cell phenotype in injured proximal tubule cell–specific ADAM17 knockout mice. Moreover, the finding that proximal tubule cell–specific amphiregulin knockout mice were protected from fibrosis after ischemia-reperfusion injury or unilateral ureteral obstruction demonstrates that amphiregulin was necessary for the development of fibrosis.ConclusionsOur results identify amphiregulin as a key player in injury-induced kidney fibrosis and suggest therapeutic or diagnostic applications of soluble amphiregulin in kidney disease.

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Visceral white adipose tissue and serum proteomic alternations in metabolically healthy obese patients undergoing bariatric surgery

Cited by 18 publications

References 46 publications

Longitudinal plasma inflammatory proteome profiling during pregnancy in the Born into Life study

Longitudinal plasma inflammatory proteome profiling during pregnancy in the Born into Life study

Relevance of Leptin and Other Adipokines in Obesity-Associated Cardiovascular Risk

Proximal Tubule–Derived Amphiregulin Amplifies and Integrates Profibrotic EGF Receptor Signals in Kidney Fibrosis

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