Visceral mesoderm signaling regulates assembly position and function of the Drosophila testis niche

Anllo, Lauren; DiNardo, Stephen

doi:10.1016/j.devcel.2022.03.009

Cited by 11 publications

(24 citation statements)

References 85 publications

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“…Indeed, a Tup::GFP transgene showed strong protein expression marking hub nuclei at the apex of adult testes ( Figure 7F–F’’’ ). This is consistent with recent evidence that Tup is expressed in and required for niche organization in the male embryonic gonad ( Anllo et al, 2019 ; Anllo and DiNardo, 2022 ). The expression of Tup in the 79 ‘true hub’ nuclei of the snRNA-seq dataset but not the other nuclei of the original 120 further validates the exclusion of the 41 dispersed nuclei previously included in cluster 90.…”

Section: Resultssupporting

confidence: 93%

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq of Drosophila testes

Raz

Vida²,

Stern

et al. 2023

eLife

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Proper differentiation of sperm from germline stem cells, essential for production of the next generation, requires dramatic changes in gene expression that drive remodeling of almost all cellular components, from chromatin to organelles to cell shape itself. Here, we provide a single nucleus and single cell RNA-seq resource covering all of spermatogenesis in Drosophila starting from in-depth analysis of adult testis single nucleus RNA-seq (snRNA-seq) data from the Fly Cell Atlas (FCA) study. With over 44,000 nuclei and 6000 cells analyzed, the data provide identification of rare cell types, mapping of intermediate steps in differentiation, and the potential to identify new factors impacting fertility or controlling differentiation of germline and supporting somatic cells. We justify assignment of key germline and somatic cell types using combinations of known markers, in situ hybridization, and analysis of extant protein traps. Comparison of single cell and single nucleus datasets proved particularly revealing of dynamic developmental transitions in germline differentiation. To complement the web-based portals for data analysis hosted by the FCA, we provide datasets compatible with commonly used software such as Seurat and Monocle. The foundation provided here will enable communities studying spermatogenesis to interrogate the datasets to identify candidate genes to test for function in vivo.

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Section: Resultssupporting

confidence: 93%

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq of Drosophila testes

Raz

Vida²,

Stern

et al. 2023

eLife

Self Cite

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“…Indeed, a Tup::GFP transgene showed strong protein expression marking hub nuclei at the apex of adult testes (Figure 7F-F'''). This is consistent with recent evidence that Tup is expressed in and required for niche organization in the male embryonic gonad (Anllo and DiNardo, 2022;Anllo et al, 2019).…”

Section: The Hub: Architectural Organizer and Key Signaling Centersupporting

confidence: 93%

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq ofDrosophilatestes

Raz

Vida²,

Stern

et al. 2022

Preprint

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Proper differentiation of sperm from germline stem cells, essential for production of the next generation, requires dramatic changes in gene expression that drive remodeling of almost all cellular components, from chromatin to organelles to cell shape itself. Here we provide a single nucleus and single cell RNA-seq resource covering all of spermatogenesis in Drosophila starting from in-depth analysis of adult testis single nucleus RNA-seq (snRNA-seq) data from the Fly Cell Atlas (FCA) study (Li et al., 2022). With over 44,000 nuclei and 6,000 cells analyzed, the data provide identification of rare cell types, mapping of intermediate steps in differentiation, and the potential to identify new factors impacting fertility or controlling differentiation of germline and supporting somatic cells. We justify assignment of key germline and somatic cell types using combinations of known markers, in situ hybridization, and analysis of extant protein traps. Comparison of single cell and single nucleus datasets proved particularly revealing of dynamic developmental transitions in germline differentiation. To complement the web-based portals for data analysis hosted by the FCA, we provide datasets compatible with commonly used software such as Seurat and Monocle. The foundation provided here will enable communities studying spermatogenesis to interrogate the datasets to identify candidate genes to test for function in vivo.

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“…Using a previously validated bnl-Gal4 (Chen and Krasnow, 2014; Kamimura et al, 2006; Spéder and Brand, 2014; Tamamouna et al, 2021), we drove CD8::GFP expression and found that it was expressed in all cells of the optic lobe (Figure 3 - Figure Supplement 1D), making it unlikely to be a viable hit. We found that a previously validated ths-Gal4 (Anllo and DiNardo, 2022; Wu et al, 2017) drove CD8::GFP expression in photoreceptors but not xg O (Figure 3 - Figure Supplement 1E) consistent with previous reports (Franzdóttir et al, 2009). However, when we examined Col4a1 expression using a previously validated Gal4 enhancer trap (Hennig et al, 2006), we found that it drove CD8::GFP expression in xg O (Figure 3 - Figure Supplement 1F).…”

Section: Resultssupporting

confidence: 92%

Differentiation signals from glia are fine-tuned to set neuronal numbers during development

Prasad

Bostock

Lago-Baldaia

et al. 2021

Preprint

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Precise neuronal numbers are required for circuit formation and function. Known strategies to control neuronal numbers involve regulating either cell proliferation or survival. In the developing Drosophila visual system photoreceptors from the eye-disc induce their target field, the lamina, one column at a time. Although each column initially contains ~6 precursors, only 5 differentiate into neurons of unique identities (L1-L5); the extra precursor undergoes apoptosis. We uncovered that Hedgehog signalling patterns columns, such that the 2 precursors experiencing the lowest signalling activity are specified as L5s; only one differentiates while the other extra precursor dies. We showed that a glial population called the outer chiasm giant glia (xgO), which reside below the lamina, relays differentiation signals from photoreceptors to induce L5 differentiation. The precursors nearest to xgO differentiate into L5s and antagonise inductive signalling to prevent the extra precursors from differentiating, resulting in their death. Thus, tissue architecture and feedback from young neurons fine-tune differentiation signals from glia to limit the number of neurons induced.

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Visceral mesoderm signaling regulates assembly position and function of the Drosophila testis niche

Cited by 11 publications

References 85 publications

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq of Drosophila testes

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq of Drosophila testes

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq ofDrosophilatestes

Differentiation signals from glia are fine-tuned to set neuronal numbers during development

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