Visceral Fat Accumulation is Associated with Oxidative Stress and Increased Matrix Metalloproteinase-9 Expression in Atherogenic Factor-overlapped Model Rats

Tomita, Yuri; Iwai, Shinichi; Kumai, Toshio; Ohnuma, Shigeko; Kurahashi, Chika; Tsuboi, Asayo; Ohba, Keiichiro; Ono, Tomohiro; Oka, Yoshiomi; Koike, Jyunki; Kobayashi, Shinichi; Oguchi, Katsuji

doi:10.15369/sujms.22.27

Cited by 6 publications

(15 citation statements)

References 45 publications

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“…The PCR reaction parameters were as follows: 95°C for 10 min, 40 cycles of 10 s at 95°C, 30 s at 60°C, and 1 s at 72°C. Fluorescence data were analyzed with LightCycler software (Roche) as described previously (35).…”

Section: Quantitation Of Mrnamentioning

confidence: 99%

Tea Polyphenols Inhibit Rat Osteoclast Formation and Differentiation

et al. 2012

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Abstract. Matrix metalloproteinases (MMPs) play an important role in degeneration of the matrix associated with bone and cartilage. Regulation of osteoclast activity is essential in the treatment of bone disease, including osteoporosis and rheumatoid arthritis. Polyphenols in green tea, particularly epigallocatechin-3-gallate (EGCG), inhibit MMPs expression and activity. However, the effects of the black tea polyphenol, theaflavin-3,3′-digallate (TFDG), on osteoclast and MMP activity are unknown. Therefore, we examined whether TFDG and EGCG affect MMP activity and osteoclast formation and differentiation in vitro. TFDG or EGCG (10 and 100 μM) was added to cultures of rat osteoclast precursors cells and mature osteoclasts. Numbers of multinucleated osteoclasts and actin rings decreased in polyphenol-treated cultures relative to control cultures. MMP-2 and MMP-9 activities were lower in TFDG-and EGCG-treated rat osteoclast precursor cells than in control cultures. MMP-9 mRNA levels declined significantly in TFDG-treated osteoclasts in comparison to control osteoclasts. TFDG and EGCG inhibited the formation and differentiation of osteoclasts via inhibition of MMPs. TFDG may suppress actin ring formation more effectively than EGCG. Thus, TFDG and EGCG may be suitable agents or lead compounds for the treatment of bone resorption diseases.

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Section: Quantitation Of Mrnamentioning

confidence: 99%

Tea Polyphenols Inhibit Rat Osteoclast Formation and Differentiation

et al. 2012

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“…In addition, oxidative stress plays an important role in the progression of NASH 13 . As we have shown in a previous study 11 , the diacron reactive oxidative metabolites d-ROMs test can be used as an indicator of oxidative stress.…”

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confidence: 66%

“…In addition, vascular intimal lesions and lipid deposits have been observed under the endothelial cells in the aorta of SHHRs, but not in spontaneously hyperlipidemic rats or controls 8 . Moreover, invasive changes occur in the subendothelium of SHHR when nitric oxide production is inactivated, followed by a high fat diet and sucrose water treatment HFDS 9 , and a previous study found visceral fat accumulation and increased oxidative stress in SHHR-HFDS 10,11 . We have found brosis and cell in ammation around the central vein, and fat accumulation in hepatocytes on liver histology in SHHR-HFDS ; thus we use SHHR-HFDS as a rat model of NASH.…”

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confidence: 88%

“…MMPs are associated with liver brosis ; in particular, MMP-2 and MMP-9 play important roles in the progression of liver brosis 15 , and MMP-9 activity and expression are associated with oxidative stress 16 . Our previous studies showed that mRNA expression of MMP-9 in white blood cells WBCs is signi cantly higher in SHHR-HFDS compared to other rats 11 . Moreover, MMPs are tightly regulated by tissue inhibitors of metalloproteinases TIMPs 17 .…”

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confidence: 89%

“…Table 1 Fluorescence data were analyzed with LightCycler software Roche . The mRNA levels were compared to 18 s rRNA as a standard, and relative expression ratios were obtained 11 .…”

Section: Quantitative Real-time Polymerase Chain Reactionmentioning

confidence: 99%

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