Viruses affecting tomato crops in Serbia

Nikolić, Dušan; Vučurović, Ana; Stanković, Ivana; Radović, Nikol; Zečević, Katarina; Bulajić, Aleksandra; Krstić, Branka

doi:10.1007/s10658-018-1467-y

Cited by 13 publications

(9 citation statements)

References 21 publications

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“…It is efficiently transmitted by Myzus persicae and other aphid species (Revers and García, 2015;Gadhave et al, 2020). PVY is widespread in several tomato-growing regions worldwide, based on recent large-scale studies (Soler et al, 2010;Al-Kuwaiti et al, 2016;Xu et al, 2017;Nikolić et al, 2018;Ignatov et al, 2020;Ma et al, 2020). Recently, PVY strain C was detected in tomatoes showing leaf necrosis in Kenya (Chikh-Ali et al, 2015).…”

Section: Potyvirusesmentioning

confidence: 99%

“…These methods are widely available in molecular biology labs, but they require a priori information on viral genomes or serological properties of viral species that might be present in a sample. The study of a whole community of viruses (i.e., viromes), including unknown ones, became possible due to the rapid decrease in high-throughput sequencing (HTS) costs (NIH-NHGRI, 2019) and the availability of the improved data analysis tools (Villamor et al, 2019). This circumvented challenges of targeted detection of plant viruses and contributed useful ecological and epidemiological insights.…”

Section: Introductionmentioning

confidence: 99%

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Global Advances in Tomato Virome Research: Current Status and the Impact of High-Throughput Sequencing

et al. 2021

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Viruses cause a big fraction of economically important diseases in major crops, including tomato. In the past decade (2011–2020), many emerging or re-emerging tomato-infecting viruses were reported worldwide. In this period, 45 novel viral species were identified in tomato, 14 of which were discovered using high-throughput sequencing (HTS). In this review, we first discuss the role of HTS in these discoveries and its general impact on tomato virome research. We observed that the rate of tomato virus discovery is accelerating in the past few years due to the use of HTS. However, the extent of the post-discovery characterization of viruses is lagging behind and is greater for economically devastating viruses, such as the recently emerged tomato brown rugose fruit virus. Moreover, many known viruses still cause significant economic damages to tomato production. The review of databases and literature revealed at least 312 virus, satellite virus, or viroid species (in 22 families and 39 genera) associated with tomato, which is likely the highest number recorded for any plant. Among those, here, we summarize the current knowledge on the biology, global distribution, and epidemiology of the most important species. Increasing knowledge on tomato virome and employment of HTS to also study viromes of surrounding wild plants and environmental samples are bringing new insights into the understanding of epidemiology and ecology of tomato-infecting viruses and can, in the future, facilitate virus disease forecasting and prevention of virus disease outbreaks in tomato.

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Section: Potyvirusesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Global Advances in Tomato Virome Research: Current Status and the Impact of High-Throughput Sequencing

et al. 2021

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“…Furthermore, as a new CMV host in Serbia, safflower has become a potential natural virus reservoir and an additional source of inoculum. Given that CMV can often appear on various plant species in Serbia, (Petrović et al, 2010;Stanković et al, 2011;Vučurović et al, 2012;Milojević et al, 2013, Milošević et al, 2015bNikolić et al, 2018) because of easy transmission in a non-persistent manner by aphids and a wide range of hosts (Garcia-Arenal & Palukaitis, 2008), constant monitoring is required regarding CMV status and their presence in our country. This is the first report on the presence of CMV on safflower in Serbia, confirmed using DAS-ELISA tests, and molecular detection by RT-PCR with specific primers.…”

Section: Virus Detection and Symptomatology In The Fieldmentioning

confidence: 99%

Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia

Milošević¹,

Ignjatov²,

Marjanović-Jeromela³

et al. 2020

Ratar i povrt

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Safflower (Carthamus tinctorius L.) is an important oilseed crop belonging to the family Asteraceae. A total of 46 safflower samples were collected from Srbobran locality (South Bačka District) in Serbia in 2015 and analysed for the presence of cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV), and lettuce mosaic virus (LMV), using commercial double-antibody sandwich (DAS)-ELISA kits. Both viruses, CMV and AMV, were detected serologically in the collected samples. None of the analysed samples was found to be positive for LMV. The presence of CMV was further confirmed by mechanical transmission to test the plants including Chenopodium quinoa, C. amaranticolor, Nicotiana glutinosa, and Datura stramonium as well as C. tinctorius, confirming the infectious nature of the disease. Molecular detection of CMV was performed by amplification of a 871 bp fragment in all the tested samples, using the specific primers CMVCPfwd/CMVCPrev that amplify the entire coat protein (CP) gene and part of 3'-and 5'-UTRs of CMV RNA 3. The RT-PCR products derived from the isolates 290Saff and 294Saff were sequenced (MH577791 and MH577792, respectively) and compared with the CMV sequences available in GenBank. Phylogenetic analysis based on CP gene sequences showed clustering of the selected isolates into three subgroups: IA, IB and II. Serbian CMV isolates found in safflower belong to subgroup II. To our knowledge, this is the first report on CMV infection of safflower in Serbia, which has the potential to cause substantial damage to safflower production and pose a threat to other economic crops grown in Serbia.

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“…This study revealed that PVY and CMV were prevalent viruses in pepper crops in Serbia, but their occurrence, distribution, and relative incidence varied during the investigation. However, the dominant presence of PVY has been observed also in tobacco (Stanković et al, 2011), and it was one of the most frequently found virus in individual and mixed infections in tomato production in Serbia (Nikolić et al, 2018). Furthermore, CMV is well established in the tomato (Nikolić et al, 2018), different pumpkin species (Vučurović et al 2012), tobacco (Stanković et al, 2011) and other hosts which serve as CMV reservoirs.…”

Section: Disease Incidence and Symptomatology In The Fieldmentioning

confidence: 99%

“…However, the dominant presence of PVY has been observed also in tobacco (Stanković et al, 2011), and it was one of the most frequently found virus in individual and mixed infections in tomato production in Serbia (Nikolić et al, 2018). Furthermore, CMV is well established in the tomato (Nikolić et al, 2018), different pumpkin species (Vučurović et al 2012), tobacco (Stanković et al, 2011) and other hosts which serve as CMV reservoirs. I 10 20 0 60 0 0 0 0 0 0 0 20 0 60 0 Horgoš II 5 0 20 0 0 60 0 0 0 20 0 80 100 20 0 Senta 20 35 15 25 0 0 0 0 0 0 0 35 15 Difficulties in controlling PVY and CMV infections are caused by the great variability of the virus, their polyphagous nature and numerous aphid vectors which transmit the virus with different efficiencies.…”

Section: Disease Incidence and Symptomatology In The Fieldmentioning

confidence: 99%

Occurrence and diversity of viruses infecting pepper in Serbia

Milošević¹,

Ignjatov²,

Stanković³

et al. 2018

Acta agriculturae Serbica

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A two-year investigation (2009-2010) of the presence and distribution of pepper (Capsicum annuum L.) viruses in Serbia revealed that viruses occur each year in open-field production. Disease incidence, as estimated by the number of symptomatic plants in the field, highly varied depending on the year and sampling locality. Disease incidence ranged from 20% to 60%. Four viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), of which PVY was predominant, were detected by serological testing of pepper samples collected from many localities in Serbia. Molecular detection of PVY was performed based on amplification of a 975 bp fragment in all tested samples, using the specific primers PVYc/PVYd that amplify the gene for P1 protein. The RT-PCR products derived from the four isolates (PL-28-09, PL-15-09, PL-3-10, PL-108-10) of PVY were sequenced (KC288142, KC288143, KC288144, and KC288144, respectively) and compared with the PVY sequences available in GenBank. Sequence analysis, conducted with MEGA5 software, revealed 99.8-100% nt identity among the four Serbian PVY isolates from pepper. The sequences of PVY isolates from Serbia share the highest nucleotide and amino acid identity with isolates from Slovenia, Croatia, Germany, and tobbaco isolate from Serbia. All of the four Serbian isolates were clustered in subgroup N-1 with other European isolates of necrotic strains.

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Viruses affecting tomato crops in Serbia

Cited by 13 publications

References 21 publications

Global Advances in Tomato Virome Research: Current Status and the Impact of High-Throughput Sequencing

Global Advances in Tomato Virome Research: Current Status and the Impact of High-Throughput Sequencing

Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia

Occurrence and diversity of viruses infecting pepper in Serbia

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