Virus Vector for Gene Silencing in Wheat

Resources for Virus-Induced Gene Silencing in the Grasses

“…A clone of BSMV was made into a vector for use in barley (Holzberg et al, 2002) and wheat (Triticum aestivum; Scofield et al, 2005;Tai et al, 2005), and currently is the most widely employed grass VIGS vector (Table I). BSMV is a positive-strand RNAvirus of the genus Hordeivirus.…”

“…Using this system, the three DNA plasmids are mixed, bombarded into barley to initiate viral infection, and then plant sap containing large amounts of infectious virus is extracted from these plants that can then be used to infect many plants for VIGS studies. A third variation of the BSMV-VIGS vector was explored by Tai et al (2005), where a BamHI restriction site for cloning plant gene fragments was engineered into the ATG codon of the gb gene. With this cloning strategy, gb is either not expressed or, if its translation can be initiated from an in frame ATG provided by the plant gene sequence, it is synthesized as a fusion protein with the N-terminal amino acids encoded by the plant gene insert.…”

Foxtail Mosaic Virus-Induced Gene Silencing in Monocot Plants

“…By contrast, to date only four RNA viruses and one DNA virus have been modified as vectors for VIGS in monocot species, of which, Barley stripe mosaic virus (BSMV)-based VIGS has been applied for functional genomics in barley (Hordeum vulgare L.) and wheat (Triticum aestivum; Holzberg et al, 2002;Tai et al, 2005;Meng et al, 2009;Pacak et al, 2010;Yuan et al, 2011); Brome mosaic virus (BMV) in rice (Oryza sativa L.), barley, and maize (Zea mays L.; Ding et al, 2006); Bamboo mosaic virus and its satellite RNA in N. benthamiana and Brachypodium distachyon (Liou et al, 2014); and Rice tungro bacilliform virus in rice (Purkayastha et al, 2010). Very recently, Cucumber mosaic virus-based VIGS in maize is reported (Wang et al, 2016) BMV and BMV-based vectors are frequently used for VIGS in some monocot plants.…”

A new BSMV-based vector with modified β molecule allows simultaneous and stable silencing of two genes

“…Out of the several VIGS vectors elaborated for these plants, the system based on Brome mosaic virus (BMV) has been relatively seldom used [5][6][7] while the system built on Barley stripe mosaic virus (BSMV) has been applied most often. BSMV has been used for virus-induced gene silencing in barley [8][9][10][11][12], wheat [13][14][15] and Brachypodium distachyon [12,16]. The complete list of other viruses adapted for VIGS and used in grasses was presented and discussed in a recent review [17].…”

“…A similar approach to constructing the BSMV vector was presented by Bruun-Rasmussen et al [19], who engineered a cloning site in the γ molecule downstream of the γb region. Another strategy was applied by Tai et al [14]. They cloned the silencing fragment in front of the γb coding sequence, which prevented the expression of the γb gene.…”