Virus-Specific Deoxyribonucleic Acid in Simian Virus 40-Exposed Hamster Cells: Correlation with S and T Antigens

Abstract

Several homologous hamster embryonic cell lines, transformed in association with simian virus (SV) 40 infection, were examined for the presence of deoxyribonucleic acid (DNA) complementary to SV40 ribonucleic acid (RNA) made in vitro. The methods employed permitted the detection of 10 −5 μg of viral DNA in 100 μg of cellular DNA, corresponding to one-fifth of an SV40 DNA molecule per cell. Those lines which contained both the SV40 surface (S) and tumor (T) antigens also contained DNA complementary to SV40 RNA synthesized in vitro. In contrast, neither of two lines which contained S, but not T, antigen contained detectable DNA complementary to SV40 RNA. These findings suggest that the production of S antigen does not depend upon the persistence of SV40 DNA in transformed cells.

Interactions of Polyoma and Mouse DNAs. IV. Time Course and Extent of Integration of Polyoma DNA into Mouse DNA During Lytic Infection

“…The values obtained for the different polyoma-transformed and tumor cells are listed in Table 3. They are comparable to results obtained by different methods for other virustransformed cell lines (9,18,26,38). e Mouse DNA (50 ug) plus polyoma DNA (2 x 10-4 pg).…”

The SV40 S antigen; A carcinoembryonic‐type antigen of the hamster?

“…These antigens were not detected in SV40-exposed hamster cells containing S, but lacking T antigen (Sq-T--) Wright, 1971). The fact that SV40-transformed mouse cells failed to show S antigen in this and a previous study (Levine et al, 1970) would indicate that anti-S antiserum might not contain the isotopic binding antibody. A more direct confirmation of this supposition is now being undertaken.…”

“…An enumeration of all cell lines used in this study is presented in Table I. H50 and P41 were previously shown to contain SV40 S as well as T antigen (S+T+) Levine et al, 1970). All lines were propagated in monolayer with Eagle's minimal essential medium (Hanks' salts) and 2 or 10% fetal bovine serum.…”

“…It was postulated that this antigen (S-antigen) may have been similar or identical to the SV40 tumor-specific transplantation antigen; possibly coded by the viral genome. More recent experimental evidence however has laid this viewpoint open to doubt: (1) Some lines of SV40-exposed hamster cells contain the S antigen, although they do not contain SV40 T antigen, SV40 tumor-specific transplantation antigen, radioimmunoassay detectable SV40-specific surface antigen or hybridizable SV40-specific RNA Tevethia et al, 1968;Wright, 1971;Levine et al, 1970); (2) the sera of pregnant hamsters have been shown to react with hamster cells containing the S antigen (Duff and Rapp, 1970); and (3) upon exposure to proteolytic enzymes, lines of non-SV40-exposed hamster cells have been shown to contain an antigen similar or identical to the SV40 S antigen (Hayry…”

Control of Simian Virus 40 Gene Expression in Adenovirus-Simian Virus 40 Hybrid Viruses Synthesis of Hybrid Adenovirus 2-Simian Virus 40 RNA Molecules in Cells Infected with a Nondefective Adenovirus 2-Simian Virus 40 Hybrid Virus

“…DISCUSSION Previous studies using RNA-DNA hybridization and heteroduplex mapping have demonstrated that Ad2+ND, contains 43% of the wild-type SV40 genome covalently linked to Ad2 DNA (11,15). This SV40 genetic information, which includes all of the early SV40 nucleotide sequences (22), is inserted as a single segment of SV40 DNA at a point approximately 14% in from one end of the Ad2 genome (15). The fact that a significant proportion of the SV40-specific RNA synthesized during Ad2+ND, infection could be hybridized to Ad2 DNA, eluted, and rehybridized to SV40 DNA (Tables 1 and 2) indicates that at least some of the SV40 RNA sequences induced by this hybrid virus are contained in polycistronic RNA molecules which also contain Ad2 nucleotide sequences.…”