Virus recovering from strawberries: Evaluation of a skimmed milk organic flocculation method for assessment of microbiological contamination

Melgaço, Fabiana Gil; Victoria, Matías; Corrêa, Adriana Abreu; Ganime, Ana Carolina; Malta, Fábio Correia; Brandão, Marcelo Luiz Lima; Medeiros, Valéria de Mello; Rosas, Carla de Oliveira; Bricio, Silvia Maria Lopes; Miagostovich, Marize Pereira

doi:10.1016/j.ijfoodmicro.2015.10.005

Cited by 20 publications

(7 citation statements)

References 40 publications

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“…Although for NoV GII.4 CTAB treatment achieved a higher recovery rate when compared to data reported previously obtained for strawberry samples. 29 In this study, we considered CTAB treatment once its use was efficient for strawberry samples. 29 CTAB is a reagent described to eliminate possible inhibitors of qPCR reaction, as organic compounds, pigments and sugars present in food samples.…”

Section: Discussionmentioning

confidence: 99%

“…Skimmed milk flocculation method was performed as described by Melgaço et al 29 including the use of cetyltrimethylammonium bromide (CTAB) ( Fig. 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…This study aims to expand previous studies that adapted successfully skimmed milk flocculation method to recover virus from strawberries. 29 Here, we assess NoV, MNV-1 and HAdV success rate and efficiency recoveries from three varieties of tomatoes as well as assess their microbiological quality by investigating NoV GI and GII, HAdV, Salmonella spp., Listeria monocytogenes and fecal coliforms from samples obtained at market places at the Great Metropolitan Region of Rio de Janeiro State.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of skimmed milk flocculation method for virus recovery from tomatoes

Melgaço

Corrêa

Ganime

et al. 2018

Brazilian Journal of Microbiology

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This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial establishments in the metropolitan region of Rio de Janeiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed with no detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated that the skimmed milk flocculation method is suitable for recovering HAdV from tomatoes and highlights the need for considering investigation in order to improve food safety.

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Section: Discussionmentioning

confidence: 99%

“…Skimmed milk flocculation method was performed as described by Melgaço et al 29 including the use of cetyltrimethylammonium bromide (CTAB) ( Fig. 1 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of skimmed milk flocculation method for virus recovery from tomatoes

Melgaço

Corrêa

Ganime

et al. 2018

Brazilian Journal of Microbiology

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“…The interactions among native Con A, mutated MCR of Con A (mCon A MCR , 16 DIGDPNYPH 24 / 16 AAAAAAAAA 24 ) and mutated carbohydrate binding region (mCon A CBR ) including Y12F, N14G, L99E, Y100F, and D208G, with HuNoV (GII.4) were measured via SPR ( Fig. 2A and C) to identify the interaction regions of Con A.…”

Section: Complex Generation Between Hunov (Gii4) and Con Amentioning

confidence: 99%

“…However, sample preparation for these methods to detect viral contamination in food and water is complicated by factors such as large sample volumes, low viral titers, and compounds that interfere with nucleic acid amplification [15]. Methods available for NoV concentration, such as swab sampling, polyethylene glycol (PEG) precipitation, ultracentrifugation, cationic separation, immune concentration, and organic flocculation, have limitations due to their narrow pH ranges, time requirements, and difficulties in handling multiple samples at a time [16,17]. Therefore, an alternative method is necessary to overcome the limitations of such methods.…”

Section: Introductionmentioning

confidence: 99%

Exploration of the metal coordination region of concanavalin A for its interaction with human norovirus

Kim

Lee

et al. 2017

Biomaterials

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Rapid methods for the detection and clinical treatment of human norovirus (HuNoV) are needed to control foodborne disease outbreaks, but reliable techniques that are fast and sensitive enough to detect small amounts of HuNoV in food and aquatic environments are not yet available. We explore the interactions between HuNoV and concanavalin A (Con A), which could facilitate the development of a sensitive detection tool for HuNoV. Biophysical studies including hydrogen/deuterium exchange (HDX) mass spectrometry and surface plasmon resonance (SPR) revealed that when the metal coordinated region of Con A, which spans Asp16 to His24, is converted to nine alanine residues (mCon A), the affinity for HuNoV (GII.4) diminishes, demonstrating that this Ca and Mn coordinated region is responsible for the observed virus-protein interaction. The mutated carbohydrate binding region of Con A (mCon A) does not affect binding affinity significantly, indicating that MCR of Con A is a major region of interaction to HuNoV (GII.4). The results further contribute to the development of a HuNoV concentration tool, Con A-immobilized polyacrylate beads (Con A-PAB), for rapid detection of genotypes from genogroups I and II (GI and GII). This method offers many advantages over currently available methods, including a short concentration time. HuNov (GI and GII) can be detected in just 15 min with 90% recovery through Con A-PAB application. In addition, this method can be used over a wide range of pH values (pH 3.0 - 10.0). Overall, this rapid and sensitive detection of HuNoV (GI and GII) will aid in the prevention of virus transmission pathways, and the method developed here may have applicability for other foodborne viral infections.

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Methods for Virus Recovery from Foods

Goyal

Aboubakr

2016

Viruses in Foods

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Virus recovering from strawberries: Evaluation of a skimmed milk organic flocculation method for assessment of microbiological contamination

Cited by 20 publications

References 40 publications

Evaluation of skimmed milk flocculation method for virus recovery from tomatoes

Evaluation of skimmed milk flocculation method for virus recovery from tomatoes

Exploration of the metal coordination region of concanavalin A for its interaction with human norovirus

Methods for Virus Recovery from Foods

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