Virus Neutralizing and Enhancing Epitopes Characterized by Synthetic Oligopeptides Derived from the Feline Leukaemia Virus Glycoprotein Sequence

Nick, Sigrid; Klaws, J.; Friebel, Klaus; Birr, Christian; Hunsmann, Gerhard; Bayer, H.

doi:10.1099/0022-1317-71-1-77

Cited by 17 publications

(14 citation statements)

References 38 publications

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“…Functional studies of the surface glycoprotein of feline leukemia virus (FeLV) have revealed specific segments that are responsible for receptor binding (11,12), specific pathogenic properties (6), and neutralization sites (10,13,14,15). Previous reports have described a clustering of neutralization sites within a proline-rich sequence located between amino acids 230-289 of gp70 (10,13,14,15).…”

Section: Introductionmentioning

confidence: 99%

“…The alternative to complete structural determination of retroviral envelope proteins has been to dissect the structurefunction relationships of these proteins through indirect means. Retroviral envelope protein structure and function has been analyzed by using the construction of chimeric viruses (4,6), site-specific mutagenesis (3,7), and studies with small synthetic peptides and specific antibodies (8,9,10), to make inferences about specific structural and functional domains.…”

Section: Introductionmentioning

confidence: 99%

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Structure and Self Assembly of a Retrovirus (FeLV) Proline Rich Neutralization Domain

Fontenot

Tjandra

et al. 1994

Journal of Biomolecular Structure and Dynamics

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The 60 amino acid proline-rich neutralization domain of the external surface unit glycoprotein of feline leukemia virus was chemically synthesized in total and in fragments. We examined the ability of these retroviral peptides to form ordered conformations using 1H-NMR, circular dichroism spectroscopy, and intrinsic viscosity measurements. One dimensional nuclear magnetic resonance spectroscopy revealed that the 60 amino acid peptide could form a stable, folded structure that was long-lived, as shown by the ability to protect amide-protons in D20. Peptides corresponding to the N-terminal 42, N-terminal 20 amino acids, and middle 20 amino acid sections could also form stable structures. The C-terminal segment did not protect any protons in D20. Interestingly, self assembly of the N-terminal 42 and C-terminal 16 amino acid peptides into a structure very close to that of the 60 amino acid domain was observed. The circular dichroism results reveals a large negative cotton effect at 198 nm that is characteristic of the proline-rich beta-turn helixes which consist predominantly of trans-proline. The intrinsic viscosity results suggest a non-random coil structure that is rod shaped. Our conclusion is that PRN60 forms a beta-turn helix and that this region of FeLV-gp70 is a separate folding domain of the retroviral surface unit glycoprotein. The unique conformational properties of PRN60 and its critical role as the predominant target for neutralizing antibody responses suggest that this peptide is a reasonable candidate for producing a synthetic peptide vaccine for FeLV.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structure and Self Assembly of a Retrovirus (FeLV) Proline Rich Neutralization Domain

Fontenot

Tjandra

et al. 1994

Journal of Biomolecular Structure and Dynamics

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“…This leads to a marrow-origin, cellfree viraemia and further tertiary replication in mucosal sites from where it is shed in high concentrations (Francis et al, 1977). Antigenic targets for the virus are less well defined although sites involved in complementmediated lysis, antibody-dependent cellular cytotoxicity of virus-infected cells (McCarty & Grant, 1983;Grant et al, 1980a) and neutralization (Osterhaus et al, 1989;deNoronha et al, 1983;Grant et al, 1980b) have been mapped to the gp70 and pI5E envelope proteins Nunberg et al, 1984;Nick et al, 1990). Studies with analogous viruses would also suggest that both the core gag and env protein can act as important immunological targets for cytotoxic T cells (Tc) (Holt et al, 1986;van der Hoorn et al, t985;Flyer et al, 1983;Manjunath et al, 1986;Martin & Rouse, 1990).…”

Section: Discussionmentioning

confidence: 99%

“…The role which this protein plays in the pathogenesis of FeLV infection is still controversial as claims for a potent immunosuppressive effect and hence exclusion from any vaccine preparation lie side by side with arguments for its inclusion in any vaccine because anti-pl 5E antibodies could have a beneficial effect on protection (Olsen et al, 1977;Mathes et al, 1978Mathes et al, , 1979. Antibody reactivity which maps to the N-terminal end of pl 5E is capable of viral neutralization (Nick et al, 1990) and thus may play a role during the systemic phase of the infection. When we compared FHVAtkgp85 plus AcNPVgp85 with FHVAtkgp70 plus AcNPVgp70, the regime which contained p 15E gave better protection indicating that in this particular format no deleterious effect of p 15E could be detected.…”

Section: Discussionmentioning

confidence: 99%

The use of feline herpesvirus and baculovirus as vaccine vectors for the gag and env genes of feline leukaemia virus

Wardley¹,

Berlinski²,

Thomsen³

et al. 1992

Journal of General Virology

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The env and gag genes from feline leukaemia virus were expressed in a thymidine kinase-negative feline herpesvirus and a baculovirus. Cats were vaccinated with various combinations of these recombinant viruses and 100% protection against feline leukaemia virus challenge was achieved using an immunization schedule which utilized both env and gag products delivered at both a mucosal and systemic site.

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“…Additional peptides were chosen from the beginning of the amino acid sequence of the MuLV glyco-Gag protein, a known epitope of murine sarcoma virus (MSV)-induced tumors (3,19,27,29,46,47). The Env peptides (SU and TM) were chosen from (i) known epitopes of the FeLV gp70 (52,70,71), (ii) the epitope of the broadly neutralizing XMRV Env monoclonal antibody 83A25 (39,45,50,51), (iii) epitopes of narrowly reacting nonneutralizing monoclonal antibodies, (iv) a major cytotoxic T lymphocyte (CTL) epitope in p15E (21,63), (v) studies on FeLV TM epitopes (37,44), and (vi) critical portions of the immunosuppressive domain as defined in MuLV (7,35,41) and analogous to antigenic peptides from HIV.…”

Section: Methodsmentioning

confidence: 99%

No Evidence for Xenotropic Murine Leukemia-Related Virus Infection in Sweden Using Internally Controlled Multiepitope Suspension Array Serology

Blomberg

Sjösten

et al. 2012

Clin Vaccine Immunol

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ABSTRACTMany syndromes have a large number of differential diagnoses, a situation which calls for multiplex diagnostic systems. Myalgic encephalomyelitis (ME), also named chronic fatigue syndrome (CFS), is a common disease of unknown etiology. A mouse retrovirus, xenotropic murine leukemia-related virus (XMRV), was found in ME/CFS patients and blood donors, but this was not corroborated. However, the paucity of serological investigations on XMRV in humans prompted us to develop a serological assay which cover many aspects of XMRV antigenicity. It is a novel suspension array method, using a multiplex IgG assay with nine recombinant proteins from theenvandgaggenes of XMRV and 38 peptides based on known epitopes of vertebrate gammaretroviruses. IgG antibodies were sought in 520 blood donors and 85 ME/CFS patients and in positive- and negative-control sera from animals. We found no differences in seroreactivity between blood donors and ME/CFS patients for any of the antigens. This did not support an association between ME/CFS and XMRV infection. The multiplex serological system had several advantages: (i) biotinylated protein G allowed us to run both human and animal sera, which is essential because of a lack of XMRV-positive humans; (ii) a novel quality control was a pan-peptide positive-control rabbit serum; and (iii) synthetic XMRV Gag peptides with degenerate positions covering most of the variation of murine leukemia-like viruses did not give higher background than nondegenerate analogs. The principle may be used for creation of variant tolerant peptide serologies. Thus, our system allows rational large-scale serological assays with built-in quality control.

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Virus Neutralizing and Enhancing Epitopes Characterized by Synthetic Oligopeptides Derived from the Feline Leukaemia Virus Glycoprotein Sequence

Cited by 17 publications

References 38 publications

Structure and Self Assembly of a Retrovirus (FeLV) Proline Rich Neutralization Domain

Structure and Self Assembly of a Retrovirus (FeLV) Proline Rich Neutralization Domain

The use of feline herpesvirus and baculovirus as vaccine vectors for the gag and env genes of feline leukaemia virus

No Evidence for Xenotropic Murine Leukemia-Related Virus Infection in Sweden Using Internally Controlled Multiepitope Suspension Array Serology

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