Virus-mediated release of endosomal content in vitro: different behavior of adenovirus and rhinovirus serotype 2.

Prchla, Elisabeth; Plank, Christian; Wagner, Ernst; Blaas, Dieter; Fuchs, Renate

doi:10.1083/jcb.131.1.111

Cited by 124 publications

(120 citation statements)

References 67 publications

Supporting

Mentioning

114

Contrasting

Unclassified

Order By: Relevance

“…168 Peptides derived from both HRV2 VP1 and influenza virus HA proteins allowed escape of low molecular weight (MW) (ie 10 kilodaltons, or kDa), but not high MW (ie 70 kDa), dextrans from endosomal vesicles. Likewise, whole HRV2 enabled endosome leakage of only the 10 kDa, but not the 70 kDa, molecule.…”

Section: Other Mechanismsmentioning

confidence: 99%

Intracellular trafficking of nonviral vectors

2005

View full text Add to dashboard Cite

Section: Other Mechanismsmentioning

confidence: 99%

Intracellular trafficking of nonviral vectors

2005

View full text Add to dashboard Cite

“…During cell entry, nonenveloped viruses have to breach a biological membrane to deliver their genomes to the cell cytoplasm. Previously, it has been shown that both myristoylated and unmodified VP4 minor capsid proteins (which are 60-80 residues long) of several picornaviruses and dicistroviruses can induce the lysis of liposomes (26,40,41). In contrast, the VP4 subunits of SBPV have been predicted to be only 20 residues long, and lack the myristoylation signal sequence (42).…”

Section: Genome Release Is Associated With Formation Of Pores At Thrementioning

confidence: 99%

Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism

Kalynych

Füzik

Přidal

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Viruses from the family Iflaviridae are insect pathogens. Many of them, including slow bee paralysis virus (SBPV), cause lethal diseases in honeybees and bumblebees, resulting in agricultural losses. Iflaviruses have nonenveloped icosahedral virions containing single-stranded RNA genomes. However, their genome release mechanism is unknown. Here, we show that low pH promotes SBPV genome release, indicating that the virus may use endosomes to enter host cells. We used cryo-EM to study a heterogeneous population of SBPV virions at pH 5.5. We determined the structures of SBPV particles before and after genome release to resolutions of 3.3 and 3.4 Å, respectively. The capsids of SBPV virions in low pH are not expanded. Thus, SBPV does not appear to form "altered" particles with pores in their capsids before genome release, as is the case in many related picornaviruses. The egress of the genome from SBPV virions is associated with a loss of interpentamer contacts mediated by N-terminal arms of VP2 capsid proteins, which result in the expansion of the capsid. Pores that are 7 Å in diameter form around icosahedral threefold symmetry axes. We speculate that they serve as channels for the genome release. Our findings provide an atomic-level characterization of the genome release mechanism of iflaviruses.electron microscopy | uncoating | honeybee | structure | virus

show abstract

“…Indeed, the routes followed by the DNA to be transcribed toward the nucleus frequently remain unclarified. As viruses used for gene transfer require internalization followed by protein modification into acidic compartments to be able to enter the cytoplasm of the infected cells, 20,21 it has been proposed that the endocytosis or phagocytosis process should be an essential step for nonviral gene transfer. 22 However, intracellular trafficking throughout endosomes and lysosomes should be different for DNA lipidic complexes, DNA polycationic complexes, or Ab-DNA conjugates.…”

mentioning

confidence: 99%

Antibody-mediated endocytosis of G250 tumor-associated antigen allows targeted gene transfer to human renal cell carcinoma in vitro

et al. 1999

View full text Add to dashboard Cite

Specific gene transfer into targeted tumor cells remains a critical issue for the development of systemic gene therapy protocols. With this end in view, we have tested the possibility of selectively directing genes to tumor cells through the recognition of tumor-associated antigens (TAA). This was approached in vitro on four human renal cell carcinoma (RCC) lines by means of the highly specific mouse G250 monoclonal antibody (mAb) chemically conjugated to a plasmid DNA conveying a reporter activity. This mAb directed to a TAA that is present on 95% of primary RCCs and on 60% of metastatic human RCCs was extensively characterized, including during clinical trials. Epifluorescence microscopy analysis indicated that upon specific binding to G250 TAA, G250 mAb alone or conjugated to plasmid DNA was internalized by an active endocytic process and colocalized with the transferrin concentrated in the late recycling perinuclear compartment. We also observed that both unconjugated G250 mAb or G250 mAb conjugated to plasmid DNA remained in the perinuclear region of the cells for Ն20 hours and were not rapidly translocated to lysosomes or recycled to the plasma membrane. In contrast, unconjugated plasmid DNA was not internalized. After transfection of G250 TAA-positive RCC lines with G250 mAb conjugated to a plasmid cDNA encoding mouse interleukin-2, a significant and sustained production of mouse interleukin-2 protein was detected from days 5-15 and was abrogated by inhibiting the internalization process. Altogether, our data showed that endocytosis of G250 TAA should be the basis of gene transfer to RCC, suggesting that targeting of TAA capable of internalization may be the basis of new approaches for designing alternative cancer gene therapy procedures.Key words: Renal cell carcinoma; tumor-associated antigen; endocytosis; antifection; gene therapy.M ost ongoing clinical trials in cancer gene therapy are based primarily on direct intratumoral administration of recombinant vectors and/or on ex vivo cell manipulations. 1 To date, only a few procedures permit cell-or tissue-specific targeting in vivo. This can be achieved with specific viral-based gene delivery systems 2,3 and nonviral approaches through DNA complexed to a ligand capable of binding the surface molecules expressed on the targeted cells. 4 -6 We have developed an approach named antifection that allows specific gene targeting. This technique is based on the use of antibodies (Abs) directed toward cell-specific receptors, which are chemically conjugated to plasmid DNA. This was first validated by using various reporter genes expressed in lymphoid cells, both in vitro and in vivo. 7,8 Recently, we have also reported the successful transfer of interleukin-3 (IL-3)-encoding plasmid DNA linked to anti-CD117 Abs into cultured human primary hemopoietic progenitors. 9 Although most of the ligands examined so far continue to be expressed on nontumor cells, limiting the tumor targeting, several Abs directed to tumor-associated antigen (TAA) present a widely restricted...

show abstract

Virus-mediated release of endosomal content in vitro: different behavior of adenovirus and rhinovirus serotype 2.

Cited by 124 publications

References 67 publications

Intracellular trafficking of nonviral vectors

Intracellular trafficking of nonviral vectors

Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism

Antibody-mediated endocytosis of G250 tumor-associated antigen allows targeted gene transfer to human renal cell carcinoma in vitro

Contact Info

Product

Resources

About