Virus-like particles in Heterosigma akashiwo (Raphidophyceae): a possible red tide disintegration mechanism

Nagasaki, Keisuke; Andô, Masashi; Imai, Ichiro; Itakura, Shigeru; Ishida, Yūzaburō

doi:10.1007/bf00349570

Cited by 95 publications

(72 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore they play important roles in nutrient (Wilhelm and Suttle 1999) and biogeochemical (Fuhrmann 1999) cycling, and influence structure and diversity of microbial and phytoplankton communities (Fuhrmann 1999, Wommack andColwell 2000). Viruses have also been observed to infect a wide range of aquatic algae (Van Etten et al, 1991, Van Etten andMeints 1999), including bloom-forming marine phytoplankton (Nagasaki et al, 1994a, 1994b, Jacobsen et al, 1996, Nagasaki and Yamaguchi 1997, Sandaa et al, 2001.…”

Section: Introductionmentioning

confidence: 99%

Pilot study of an EST approach of the coccolithophorid Emiliania huxleyi during a virus infection

Kegel

Allen

Metfies

et al. 2007

Gene

View full text Add to dashboard Cite

Blooms of the coccolithophorid Emiliania huxleyi can be infected by viruses, which can lead to bloom-termination. This pilot study used an expressed sequence tag (EST) approach to get a first view of gene-expression changes that occur during viral infection of E. huxleyi. cDNA libraries were constructed from uninfected cultures and 6, 12, and 24 h after infection with E. huxleyi-specific virus 86 (EhV-86). From each library 60-90 ESTs were randomly selected and annotated manually with PhyloGena. Viral genes were identified using BLAST-Search of the known viral genome. The data of this study show, that 6 h after viral infection the algal transcriptome changed significantly although few viral transcripts were present. At this point, changes mainly concerned transcripts related to photosynthesis and protein metabolism. However, after 24 h viral transcripts were most abundant. Viral transcripts found at this stage of viral infection encode proteins involved in protein degradation, nucleic acid degradation, transcription and replication.

show abstract

Section: Introductionmentioning

confidence: 99%

Pilot study of an EST approach of the coccolithophorid Emiliania huxleyi during a virus infection

Kegel

Allen

Metfies

et al. 2007

Gene

View full text Add to dashboard Cite

show abstract

“…The impact and abundance of viruses which infect marine microalgae were not recognised until recent years. Indications of virus infection in microalgae have been obtained with transmission electron microscopy (TEM) preparations of planktonic material collected from seawater (Proctor & Fuhrman 1991, Nagasaki et al 1994. The electron micrographs showed heavily stained virus-like particles (VLPs) inside the cells of several groups of marine microalgae and bacteria.…”

Section: Introductionmentioning

confidence: 99%

Seasonal abundance in Skagerrak-Kattegat coastal waters and host specificity of viruses infecting the marine photosynthetic flagellate Micromonas pusilla

Sahlsten¹

1998

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

Seawater sampled in the Skagerrak and Kattegat coastal waters during the period October 1995 to September 1996 were screened for the occurrence of viruses lytic to marine microalgae.Viruses lytic to the photosynthetic marine picoflagellate Adicrornonas pusilla (Butcher) Manton & Parke (Prasinophyceae) were detected in all seawater samples screened Evidence for viral lysis of any other of the 11 algal species tested was not obtained. Several viruses infecting different strains of Ad, pusilla were isolated. Ten isolated viruses which were tested for host speclficity were found to 'be species specific to M. pusilla and even strain specific to 1-3 of the 6 strains of M. pusilla used in the experiment. In the Skagerrak and Kattegat the seasonal abundance of viruses infectious to a M. pusilla strain isolated from the Oslofjord, Norway, was at least 1 order of magnitude higher (average 2.5 X 1 0 ' I-') than viruses infecting 2 M. pusilla strains isolated from Gulf of Maine, USA (average 2.2 X 104 and 4.6 X 103 I-', respectively).

show abstract

“…Both marine bacteriophages and algal viruses have been identified in marine systems (Cochlan et al 1993;Dodds 1979;Van Etten et al 1991). Lytic viruses have been described that are species specific for both bloomforming (Bratbak et al 1993;Suttle and Chan 1993;Milligan and Cosper 1994;Nagasaki et al 1994) and nonbloomforming phytoplankton (Cottrell and Suttle 1991;Suttle and Chan 1995). Estimates indicate that 3% of global primary production is lost to viral lysis (Suttle 1994), and experimental viral additions to seawater have reduced primary productivity rates by 80% (Suttle et al 1990).…”

mentioning

confidence: 99%

Release and bioavailability of C, N, P Se, and Fe following viral lysis of a marine chrysophyte

Gobler

Hutchins

Fisher

et al. 1997

Limnology & Oceanography

297

231

View full text Add to dashboard Cite

The potential importance of the viral lysis of phytoplankton for nutrient and carbon cycling has been acknowledged, but no quantitative assessments of this phenomenon exist. Radiotracer experiments examined the release and bioavailability of C, N, P Fe, and Se following viral lysis of the "brown tide" chrysophyte Aureococcus anophagefSeerens. Photochemical effects on the dissolved-particulate partitioning and biological uptake of virally released elements were also investigated. Viral lysis of A. anophagefSerens released 50% more C and Se than uninfected control cells to the dissolved phase, while N, P, and Fe remained in the particulate phase. There was a significant inverse correlation between A. unophugefirens and bacterial densities, as well as an increase in particulate organic nitrogen levels in cultures during viral lysis. These observations indicate thitt released dissolved organic matter supported bacterial growth and may be a pathway by which various elements are diverted in microbial food webs. Dissolved nutrients released by viral lysis were accumulated to varying degrees by natural assemblages of marine bacteria and cultured diatoms, and virally regenerated N and P relieved diatom nutrient limitation. During a 4-wk incubation, 80% of C and P within cell lysis debris was released to the diss,olved phase, likely due to bacterial activity. Photochemical degradation of cell lysis debris enhanced dissolved levels of Se (100%) and Fe (50%) and reduced total dissolved C by 15%. Photochemistry doubled the bioavailability of virally released Se to diatoms, while decreasing the bioavailability of C to bacteria threefold. The viral lysis of an A. unophugefirens bloom in the field could release 40 PM dissolved organic carbon and rapidly transfer other released elements to bacteria. Such occurrences may significantly affect water column chemistry, species composition, and succession within marine plankton communities.

show abstract

Virus-like particles in Heterosigma akashiwo (Raphidophyceae): a possible red tide disintegration mechanism

Cited by 95 publications

References 11 publications

Pilot study of an EST approach of the coccolithophorid Emiliania huxleyi during a virus infection

Pilot study of an EST approach of the coccolithophorid Emiliania huxleyi during a virus infection

Seasonal abundance in Skagerrak-Kattegat coastal waters and host specificity of viruses infecting the marine photosynthetic flagellate Micromonas pusilla

Release and bioavailability of C, N, P Se, and Fe following viral lysis of a marine chrysophyte

Contact Info

Product

Resources

About