Virus-like particles associated with porcine epidemic diarrhoea

Chasey, D.; Cartwright, Sheila F.

doi:10.1016/s0034-5288(18)32994-1

Cited by 157 publications

(115 citation statements)

References 4 publications

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“…PED was first reported in Belgium [3] and the United Kingdom [4] in 1978. It has subsequently been reported in many other swine-producing countries throughout the world.…”

Section: Introductionmentioning

confidence: 99%

Genetic variation analysis of reemerging porcine epidemic diarrhea virus prevailing in central China from 2010 to 2011

et al. 2012

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Porcine epidemic diarrhea has re-emerged with devastating impact in central China since October 2010. To investigate and analyze the reason of this outbreak, the M and ORF3 genes of 15 porcine epidemic diarrhea viruses (PEDV), which were collected from different areas of central China during October 2010 and December 2011, were amplified by reverse transcriptase polymerase chain reaction, cloned, sequenced, and analyzed. Sequence analyses showed that the nucleotides and amino acids were changed at some sites in the M and ORF3 genes of the 15 PEDV strains compared with those genes of CV777 reference strain. Based on the phylogenetic analyses, PEDVs in central China and reference strains could be separated into three groups: G1, G2, and G3. The 15 PEDV strains belonged to G3 group and showed a close relationship with Korean strains (2007), Thai strains (2007–2008), and partial other Chinese strains (2010–2011), but differed genetically from European strains (Br1/87) and the vaccine strain (CV777 vs) being used in China. Furthermore, all 15 PEDV strains from central China and some other isolates in China from 2003 to 2007 (LJB-03, QH, and LZC) belonged to different group. Therefore, PEDV exhibits rapid variation and genetic evolution, and the currently prevailing PEDV strains in central China are a new genotype.

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“…PED was first reported in Belgium [3] and the United Kingdom [4] in 1978. It has subsequently been reported in many other swine-producing countries throughout the world.…”

Section: Introductionmentioning

confidence: 99%

Genetic variation analysis of reemerging porcine epidemic diarrhea virus prevailing in central China from 2010 to 2011

et al. 2012

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“…PEDV has been classified as a member of the Coronaviridae family [1,2]. PED was first detected in Belgium [3] and the UK [4] in 1978, and outbreaks of this disease have been reported in many pig farming countries, leading to severe economic losses in Canada [5], Europe [6,7], and Asia, including Japan [8], China [9], Korea [10], and more recently, Thailand [11]. The complete sequence of the entire genome of the strain CV777 was found to be 28,033 nucleotides in length, after excluding the poly(A) tail [12,13].…”

Section: Introductionmentioning

confidence: 99%

Expression of a Cholera Toxin B Subunit-Neutralizing Epitope of the Porcine Epidemic Diarrhea Virus Fusion Gene in Transgenic Lettuce (Lactuca sativa L.)

2010

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Transgenic plants have been used as a safe and economic expression system for the production of edible vaccines. A synthetic cholera toxin B subunit gene (CTB) was fused with a synthetic neutralizing epitope gene of the porcine epidemic diarrhea virus (sCTB-sCOE), and the sCTB-sCOE fusion gene was introduced into a plant expression vector under the control of the ubiquitin promoter. This plant expression vector was transformed into lettuce (Lactuca sativa L.) using the Agrobacterium-mediated transformation method. Stable integration and transcriptional expression of the sCTB-sCOE fusion gene was confirmed using genomic DNA PCR analysis and northern blot analysis, respectively. The results of western blot analysis with anti-cholera toxin and anti-COE antibody showed the synthesis and assembly of CTB-COE fusion protein into oligomeric structures with pentameric sizing. The biological activity of CTB-COE fusion protein to its receptor, G(M1)-ganglioside, in transgenic plants was confirmed via G(M1)-ELISA with anti-cholera toxin and anti-COE antibody. Based on G(M1)-ELISA, the expression level of CTB-COE fusion proteins reached 0.0065% of the total soluble protein in transgenic lettuce leaf tissues. Transgenic lettuce successfully expressing CTB-COE fusion protein will be tested to induce efficient immune responses against porcine epidemic diarrhea virus infection by administration with raw material.

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“…PEDV was first detected in Belgium and the United Kingdom in 1978 (2, 3), and has since been identified in Hungary, Italy, China, and South Korea (4–7). In 2013, PEDV spread throughout the pig population in the United States, and the viral genome showed a close relationship with that of variant strains identified in China (8).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of K14JB01, a Novel Variant Strain of Porcine Epidemic Diarrhea Virus in South Korea

et al. 2014

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Virus-like particles associated with porcine epidemic diarrhoea

Cited by 157 publications

References 4 publications

Genetic variation analysis of reemerging porcine epidemic diarrhea virus prevailing in central China from 2010 to 2011

Genetic variation analysis of reemerging porcine epidemic diarrhea virus prevailing in central China from 2010 to 2011

Expression of a Cholera Toxin B Subunit-Neutralizing Epitope of the Porcine Epidemic Diarrhea Virus Fusion Gene in Transgenic Lettuce (Lactuca sativa L.)

Complete Genome Sequence of K14JB01, a Novel Variant Strain of Porcine Epidemic Diarrhea Virus in South Korea

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