Virus-Like Particle Vaccine Induces Protective Immunity against Homologous and Heterologous Strains of Influenza Virus

Quan, Fu‐Shi; Huang, Chunzi; Compans, Richard W.; Kang, Sang-Moo

doi:10.1128/jvi.02052-06

Cited by 285 publications

(400 citation statements)

References 44 publications

(42 reference statements)

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“…Influenza virus specific antibodies were determined in sera by enzyme-linked immunosorbent assay (ELISA) as described previously [11]. As coating antigens to measure virus specific antibodies, egg-grown inactivated influenza viruses (A/ PR8, A/WSN, A/Aichi, or A/Philippines) were coated onto 96-well microtiter plates (Nunc Life Technologies, Rochester, NY.)…”

Section: Evaluation Of Humoral Immune Responsesmentioning

confidence: 99%

“…Lung viral titers and neutralization assays were performed using MDCK cells as previously described [11]. Cytokine ELISA was performed as described previously [11,17].…”

Section: Lung Viral Titers Virus Neutralization and Cytokine Assaysmentioning

confidence: 99%

“…Lung viral titers and neutralization assays were performed using MDCK cells as previously described [11]. Cytokine ELISA was performed as described previously [11,17]. Ready-Set-Go IL-6 and IFN-γ kits (eBioscience, San Diego, CA) were used for detecting cytokine levels in lung extracts following the manufacturer's procedures.…”

Section: Lung Viral Titers Virus Neutralization and Cytokine Assaysmentioning

confidence: 99%

“…VLP vaccines for viruses such as hepatitis B virus and human papillomavirus are safe for broad and repeated application [4][5][6]. In recent studies, immunization of mice with monovalent influenza VLPs reduced challenge virus replication and conferred protection against relatively low doses of viral challenges [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies demonstrated that monovalent influenza VLPs could be a promising alternative influenza vaccine [7][8][9][10][11]. However, the immune responses to multivalent influenza VLP vaccine have not been investigated despite its critical significance in evaluating the alternative influenza vaccine.…”

Section: Introductionmentioning

confidence: 99%

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A bivalent influenza VLP vaccine confers complete inhibition of virus replication in lungs

Quan

Steinhauer

Huang

et al. 2008

Vaccine

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The conventional egg-grown influenza vaccines are trivalent. To test the feasibility of using multivalent influenza virus-like particles (VLPs) as an alternative influenza vaccine, we developed cell-derived influenza VLPs containing the hemagglutinin (HA) of the H1 subtype virus A/PR/ 8/34 or the H3 subtype virus A/Aichi/2/68. Mice immunized intramuscularly with bivalent influenza VLPs containing H1 and H3 HAs induced neutralizing activities against the homologous and closely related H1N1 strains A/PR/8/34 and A/WSN/33 as well as the H3N2 strains A/Aichi/ 2/68 and A/Hong Kong/68, but not the A/Philippines/2/82 strain isolated 14 years later. HA sequence and structure analysis indicated that antigenic distance could be a major factor in predicting cross-protection by VLP vaccines. The bivalent influenza VLP vaccine demonstrated advantages in broadening the protective immunity after lethal challenge infections when compared to a monovalent influenza VLP vaccine. High levels of the inflammatory cytokine IL-6 were observed in naïve or unprotected immunized mice but not in protected mice upon lethal challenge. These results indicate that multivalent influenza VLP vaccines can be an effective strategy for developing safe and alternative vaccine to control the spread of influenza viruses.

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Section: Evaluation Of Humoral Immune Responsesmentioning

confidence: 99%

“…Lung viral titers and neutralization assays were performed using MDCK cells as previously described [11]. Cytokine ELISA was performed as described previously [11,17].…”

Section: Lung Viral Titers Virus Neutralization and Cytokine Assaysmentioning

confidence: 99%