Virus‐like particle of Macrobrachium rosenbergii nodavirus produced in Spodoptera frugiperda (Sf9) cells is distinctive from that produced in Escherichia coli

Kueh, Chare Li; Yong, Chean Yeah; Dezfooli, Seyedehsara Masoomi; Bhassu, Subha; Tan, Soon Guan

doi:10.1002/btpr.2409

Cited by 24 publications

(28 citation statements)

References 33 publications

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“…Moreover, high pH (2–12) and thermal (up to 45°C) stability were reported for recombinant MrNv‐VLPs produced in a eukaryotic system (Kueh et al . ). Other recombinant VLPs structures have been produced in eukaryotic systems (Lin et al .…”

Section: Applications Of Encapsulation Technology In Aquaculture – Camentioning

confidence: 97%

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Encapsulation for delivering bioactives in aquaculture

Dezfooli

Gutierrez‐Maddox

Seyfoddin

2018

Reviews in Aquaculture

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The exploitation of wild fisheries has driven the seafood industry to develop more sustainable resources and high-intensity aquaculture. However, high-density aquaculture is prone to outbreaks of diseases. The use of chemicals and antimicrobial drugs in aquaculture is common practice, but such chemicals can have serious detrimental environmental impacts and antibiotics may result in bacterial resistance in many species. The development of non-antibiotic and green strategies, such as using probiotics and immunostimulants, aims for a more sustainable practice for health maintenance in aquaculture. Although the efficacy of such strategies has been demonstrated in cultivations settings, the effective delivery of bioactives remains a challenge. Conventional delivery methods are often ineffective in terms of dosage, stability and species specificity, and can possibly cause widespread environmental contamination. However, recently developed drug delivery systems may provide innovative ways to improve delivery with minimal waste and improved environmental protection. Indeed, such cutting-edge drug delivery systems have already provided measurable benefits in human medicine over the past two decades. New technologies, such as encapsulation and controlled release systems, can be used readily in scaled-up operations to improve the delivery of bioactives and ultimately increase production and profitability in aquaculture. This review critically analyses the use of encapsulation technology with a focus on microparticles for delivery of bioactive agents to farmed aquatic animals. This review discusses various potential encapsulation materials, their properties and a range of methods that can be applied and scaled-up for aquaculture. Finally, practical considerations for designing an efficient delivery system for aquaculture are discussed.

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Section: Applications Of Encapsulation Technology In Aquaculture – Camentioning

confidence: 97%

“…) and eukaryotic (Kueh et al . ) systems and proved the capability of VLPs in packaging both RNA and DNA molecules. Moreover, high pH (2–12) and thermal (up to 45°C) stability were reported for recombinant MrNv‐VLPs produced in a eukaryotic system (Kueh et al .…”

Section: Applications Of Encapsulation Technology In Aquaculture – Camentioning

confidence: 99%

Encapsulation for delivering bioactives in aquaculture

Dezfooli

Gutierrez‐Maddox

Seyfoddin

2018

Reviews in Aquaculture

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“…Apart from the MrNV VLPs produced in E. coli, we have also produced the MrNV capsid protein in Sf9 cells through baculovirus expression system (Kueh et al, 2017). This eukaryotic produced MrNV capsid protein self-assembles into VLPs significantly larger than their prokaryotic counterparts.…”

Section: Virus-like Particlesmentioning

confidence: 99%

Peer Review #2 of "Advances in the study of nodavirus (v0.2)"

2017

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Nodaviruses are small bipartite RNA viruses which belong to the family of Nodaviridae. They are categorized into alpha-nodavirus which infects insects and beta-nodavirus which infects fishes. Another distinct group of nodavirus infects shrimps and prawns, which has been proposed to be categorized as gamma-nodavirus. Our current review focuses mainly on recent studies performed on nodaviruses. Nodavirus can be transmitted vertically and horizontally. Recent outbreaks have been reported in China, Indonesia, Singapore and India, affecting the aquaculture industry. It also decreased mullet stock in the Caspian Sea. Histopathology and transmission electron microscopy (TEM) are used to examine the presence of nodaviruses in infected fishes and prawns. For classification, virus isolation followed by nucleotide sequencing are required. In contrast to partial sequence identification, profiling the whole transcriptome using next generation sequencing (NGS) offers a more comprehensive comparison and characterization of the virus. For rapid diagnosis of nodavirus, assays targeting the viral RNA based on reverse-transcription PCR (RT-PCR) such as microfluidic chips, reverse-transcription loop-mediated isothermal amplification (RT-LAMP) and RT-LAMP coupled with lateral flow dipstick (RT-LAMP-LFD) have been developed. Besides viral RNA detections, diagnosis based on immunological assays such as enzyme-linked immunosorbent assay (ELISA), immunodot and western blotting have also been reported. In addition, immune responses of fish and prawn are also discussed. Overall, in fish, innate immunity, cellular type I interferon immunity and humoral immunity cooperatively prevent nodavirus infections. Whereas prawns and shrimps adopt different immune mechanisms against nodavirus infections, through upregulation of superoxide anion, prophenoloxidase, superoxide dismutase (SOD), crustin, peroxinectin, anti-lipopolysaccharides and heat shock proteins (HSP). Potential vaccines for fishes and prawns based on inactivated viruses, recombinant proteins or DNA, either delivered through injection, oral feeding or immersion, are also discussed in detail. Lastly, a comprehensive review on nodavirus virus-like particles (VLPs) is presented. In recent

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“…Recombinant MrNV capsid protein produced in Escherichia coli and Spodoptera frugiperda (both Sf9 and Sf21) assembles into icosahedral virus-like particles (VLPs) although these were found to be morphologically different under transmission electron microscopy analysis [14,15]. Negative stained VLPs expressed in E. coli and Sf9 were measured to have diameters of ~30 and 40 nm, respectively [14,15]. The self-assembly/disassembly capability of the MrNV capsid protein has been exploited to develop nano-carriers for DNA and double stranded RNA [16,17].…”

Section: Introductionmentioning

confidence: 99%

Cryo-Electron Microscopy Structure of theMacrobrachium rosenbergiiNodavirus Capsid at 7 Angstroms Resolution

Kueh

Beh

et al. 2017

Preprint

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White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail disease (WTD) are associated with up to 100% mortality rates. Recombinant expression of the capsid protein of MrNV in insect cells leads to the production of VLPs closely resembling the native virus. We have investigated the structure of MrNV VLPs by cryogenic electron microscopy, determining a structure of the viral capsid at 7 angstroms resolution. Our data show that MrNV VLPs package nucleic acids in a manner reminiscent of other known nodavirus structures. The structure of the capsid however shows striking differences from insect and fish infecting nodaviruses, which have been shown to assemble trimer-clustered T=3 icosahedral virus particles. MrNV particles have pronounced dimeric blade-shaped spikes extending up to 6 nm from the outer surface of the capsid shell.Our structural analysis supports the assertion that MrNV along with the related virus of marine shrimp Penaeus vannamei nodavirus (PvNV) may represent a new genus of the Nodaviridae.

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Virus‐like particle of Macrobrachium rosenbergii nodavirus produced in Spodoptera frugiperda (Sf9) cells is distinctive from that produced in Escherichia coli

Cited by 24 publications

References 33 publications

Encapsulation for delivering bioactives in aquaculture

Encapsulation for delivering bioactives in aquaculture

Peer Review #2 of "Advances in the study of nodavirus (v0.2)"

Cryo-Electron Microscopy Structure of theMacrobrachium rosenbergiiNodavirus Capsid at 7 Angstroms Resolution

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