Virus-Induced Gene Silencing in Diploid and Tetraploid Potato Species

Zhao, Jinping; Jiang, Haolang; Wang, Guanyu; Wang, Zonghua; Dong, Jingao; Song, Junqi

doi:10.1007/978-1-0716-0751-0_4

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Dicotyledon Solanaceae1

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2021

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Cited by 2 publications

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“…Functional analysis of antiviral resistance signaling pathways has also been demonstrated by TRV-VIGS in N. benthamiana (Hashimoto et al, 2019). The TRVmediated VIGS system was established in 4-week-old in vitro diploid potato plants by syringe infiltration (Zhao et al, 2020). Additionally, TRV-and PVX-based microRNA silencing (VbMS) approaches to silence endogenous miRNAs in N. benthamiana and tomato plants by Agrobacterium infiltration have also been developed, thus facilitating functional studies of miRNAs in plants (Zhao et al, 2020).…”

Section: Dicotyledon Solanaceaementioning

confidence: 99%

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

et al. 2021

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As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements.

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Section: Dicotyledon Solanaceaementioning

confidence: 99%