Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata Permeability

Lazareva, Ekaterina A.; Atabekova, Anastasia K.; Lezzhov, Alexander A.; Morozov, Sergey Y.; Heinlein, Manfred; Solovyev, A.G.

doi:10.1007/978-1-0716-2132-5_23

Cited by 2 publications

(1 citation statement)

References 33 publications

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“…To analyze the functional significance of BMB1-BMB2 interaction mediated by the BMB1 C-terminal region, we used a complementation test with PVX-POL-GFP, a Potato virus X (PVX) genome-derived reporter construct [36]. PVX-POL-GFP replicates and expresses GFP in initially infected cells but is deficient in cell-to-cell movement as the PVX MP and capsid protein genes are not present in this construct.…”

Section: Bmb1 Region Involved In Interaction With Bmb2mentioning

confidence: 99%

Interaction between Movement Proteins of Hibiscus green spot virus

Atabekova

Lazareva

Lezzhov

et al. 2022

Viruses

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Movement proteins (MPs) of plant viruses enable the translocation of viral genomes from infected to healthy cells through plasmodesmata (PD). The MPs functions involve the increase of the PD permeability and routing of viral genome both to the PD entrance and through the modified PD. Hibiscus green spot virus encodes two MPs, termed BMB1 and BMB2, which act in concert to accomplish virus cell-to-cell transport. BMB1, representing an NTPase/helicase domain-containing RNA-binding protein, localizes to the cytoplasm and the nucleoplasm. BMB2 is a small hydrophobic protein that interacts with the endoplasmic reticulum (ER) membranes and induces local constrictions of the ER tubules. In plant cells, BMB2 localizes to PD-associated membrane bodies (PAMBs) consisting of modified ER tubules and directs BMB1 to PAMBs. Here, we demonstrate that BMB1 and BMB2 interact in vitro and in vivo, and that their specific interaction is essential for BMB2-directed targeting of BMB1 to PAMBs. Using mutagenesis, we show that the interaction involves the C-terminal BMB1 region and the N-terminal region of BMB2.

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Section: Bmb1 Region Involved In Interaction With Bmb2mentioning

confidence: 99%