Virus-Free Improved Food in the Era of Bacterial Immunity

Roy, Anirban; Singh, Aditi; Jailani, A. Abdul Kader; Gupta, Dinesh; Voloudakis, Andreas E.; Mukherjee, Sunil Kumar

doi:10.1007/978-3-030-63372-1_3

Cited by 1 publication

(1 citation statement)

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“…Scientists used CRISPR to edit UbEF1B and CCR4/NOT3, which are crucial for the replication of geminiviruses. The edited plants exhibited increased resistance to specific plant viruses [167]. Similarly, the editing of the translation initiation factor, the eIF4E1 gene in tomato plants, leads to enhanced resistance to infection by certain RNA viruses, including the pepper mottle virus [168].…”

Section: Crispr Interference (Crispri)mentioning

confidence: 99%

Dissecting Diagnostic and Management Strategies for Plant Viral Diseases: What Next?

Devi,

Guruprasath,

Balu

et al. 2024

Agriculture

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Recent advancements in molecular biology have revolutionized plant disease diagnosis and management. This review focuses on disease diagnosis through serological techniques, isothermal amplification methods, CRISPR-based approaches, and management strategies using RNA-based methods. Exploring high-throughput sequencing and RNA interference (RNAi) technologies like host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS), this review delves into their potential. Despite the precision offered by RNAi in pest and pathogen management, challenges such as off-target effects and efficient dsRNA delivery persist. This review discusses the significance of these strategies in preventing aphid-mediated plant virus transmission, emphasizing the crucial role of meticulous dsRNA design for effective viral RNA targeting while minimizing harm to plant RNA. Despite acknowledged challenges, including off-target effects and delivery issues, this review underscores the transformative potential of RNA-based strategies in agriculture. Envisaging reduced pesticide dependency and enhanced productivity, these strategies stand as key players in the future of sustainable agriculture.

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Section: Crispr Interference (Crispri)mentioning

confidence: 99%