Virus dose-dependent neutrophil and lymphocyte proportions in peripheral blood during influenza A infection of mice

Kostolanský, F; Dugovičová, V; Janulíková, J; Mucha, V; Mistríková, J; Varečková, E

doi:10.4149/av_2013_04_474

Cited by 4 publications

(6 citation statements)

References 4 publications

(4 reference statements)

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“…Leukopenia was described before as a characteristic sign accompanying the IAV infection, depending on the virulence of IAV (Kostolanský et al, 2013). We showed here that IAV infection led to a more substantial leukopenia in persistently infected mice than in naïve mice.…”

Section: Discussionsupporting

confidence: 61%

“…Our results showed that the acute IAV infection of MHV-68 persistently infected individuals triggers the alteration of the parameters of innate immunity, which can result in changes of the course of influenza infection and, consequently, in changes of pathogenesis of acute respiratory infection or in reactivation of herpesvirus from latency. The impact of such co-infection on leukocyte number and the proportion of their subpopulation could be dependent on the IAV virulence and on infectious dose of IAV, as we showed in the case of simple IAV infection (Kostolanský et al, 2013), but it could depend also on the HA subtype or host origin of IAV virus, which needs to be elucidated in future studies.…”

Section: Resultsmentioning

confidence: 82%

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The influence of dual infection with herpes and influenza viruses on the differential blood cell count of mice

Ančicová¹,

Dugovičová²,

Briestenská³

et al. 2016

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Based on our previous results, which confirmed the role of latent gammaherpesvirus infection in alteration of immune homeostasis, we studied the influence of simultaneous infection with gammaherpes and influenza viruses on selected parameters of innate immunity, particularly on the subpopulations of peripheral blood cell leukocytes. The aim was to analyze changes of differential blood cell count of BALB/c mice persistently infected with murine gammaherpesvirus 68 (MHV-68) and subsequently co-infected with influenza A virus (IAV), in comparison to mice infected with MHV-68 or with IAV only. Our results showed that ongoing gammaherpesvirus latency in mice caused a decreased number of leukocytes after acute infection with IAV in comparison to a single acute IAV infection. However, increased proportion of neutrophils was measured in peripheral blood of IAV-infected and co-infected mice. Dual infection had no effect on the proportion of monocytes or basophilic and eosinophilic granulocytes. The number of atypical lymphocytes, usually accompanying the persistent infection with MHV-68, decreased in co-infected mice as a consequence of the acute infection with IAV. Persistent infection with gammaherpesvirus may thus modulate the host immune response to influenza A virus and the acute IAV infection can influence the immune homeostasis established by latent MHV-68 infection.

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Section: Discussionsupporting

confidence: 61%

Section: Resultsmentioning

confidence: 82%

The influence of dual infection with herpes and influenza viruses on the differential blood cell count of mice

Ančicová¹,

Dugovičová²,

Briestenská³

et al. 2016

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“…Many studies aiming to identify the roles of diff erent subpopulation of leukocytes during the infl uenza infection were performed. In addition to macrophages and NK cells, neutrophils are also recruited into the lungs during infl uenza virus infection and play an important role in the immunopathology (Xu et al, 2006;Deng et al, 2010;Kostolanský et al, 2013). However, the role of neutrophils in infl uenza virus infection seems to be ambivalent.…”

Section: Discussionmentioning

confidence: 99%

“…However, the role of neutrophils in infl uenza virus infection seems to be ambivalent. Two reports showed that the depletion of neutrophils before infl uenza virus infection resulted in higher mortality rates in mice (Ito et al, 2011;Fujisawa 2008), while other reports showed that increased numbers of neutrophils in lungs during infl uenza infection were associated with severe pneumonia and high mortality (Saito et al, 2013;Kostolanský et al, 2013;Perrone et al, 2008). Th e course of infection and the role of neutrophils within it appear to be dependent on the virus virulence.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous infection with gammaherpes and influenza viruses enhances the host immune defense

Ančicová¹,

Wagnerová²,

Janulíková³

et al. 2015

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Summary. -We have studied the impact of simultaneous infection of mice with murine gammaherpesvirus (MHV) and infl uenza A virus (IAV) on the immune response and pathogenesis of both infections. Aft er a persistent MHV-68 herpesviral infection had been established, the same mice were super-infected with IAV. Individual parameters of MHV infection (viral DNA detection in organs and blood) and numbers of leukocytes in lungs and spleens were determined. With regard to the assumed reactivation of MHV-68 (mainly in lungs, spleen, thymus and peritoneal exudate cells) we focused our attention on the detection of transcripts, typical either for lytic infection (ORF50) and/or for latency (ORF73). Herpesviral DNA was detected in above mentioned organs in several intervals during the acute phase of IAV co-infection, but the expression of monitored transcripts was lower, i.e. it has decreased. Th ough the reason for such limited expression during acute infl uenza superinfection remains unclear, it is unambiguous that lower MHV-68 expression was detected in lungs and peritoneal exudate cells (PECs) from 3 rd to 10 th day aft er co-infection with IAV. Furthermore, our study showed that the ongoing gammaherpesvirus latency in co-infected mice aff ected the number of cytotoxic T-lymphocytes and neutrophils during the acute IAV infection and lowered their deviations from that of non-infected mice. Th erefore, we suppose that co-infection with herpes and infl uenza viruses could be mutually benefi cial for the host by promoting its defense against both viruses.

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“…DBA/2J mice also mount a hyper-inflammatory response with much stronger up-regulation of many immune response-dependent genes. As exemplified by the aforementioned studies, work in murine models of IAV infection have concentrated on mRNA expression studies, and common peripheral blood cells have been assessed only rarely [5–7]. In a separate study, we have recently assessed immunological and hematological parameters as indicators for disease severity in inbred mice infected with IAV strains of differential virulence [8].…”

Section: Introductionmentioning

confidence: 99%

Hematological parameters in the early phase of influenza A virus infection in differentially susceptible inbred mouse strains

et al. 2015

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BackgroundHematological parameters have not received much attention in small animal models of infection, particularly at very early time points. We therefore studied changes in leukocyte and thrombocyte numbers in a mouse model of influenza A virus (IAV) infection, including measurements within the first 24 h after infection, and also assessing effects, if any, of the infection/anesthesia procedure on these parameters.MethodsDBA/2J and C57BL/6J mice (n = 5–8 per observation) were evaluated in a time course experiment of IAV infection, focusing on early time points. After anesthesia with ketamine/xylazine, a suspension of 2 × 103 focus forming units of the mouse-adapted IAV strain A/Puerto Rico/8/1934 (H1N1) in 20 µl sterile PBS, or 20 µl sterile PBS only (“mock treatment”), were instilled intranasally. Weight loss was assessed daily, and eight common hematological parameters and viral hemagglutinin (HA) mRNA expression were determined after 6, 12, 18, 24, 48 and 120 h.ResultsHematological differences between the strains were apparent even in untreated mice. Infection-dependent changes, in particular increased granulocyte and decreased lymphocyte counts, were first detectable after 18 h in DBA/2J, were fully manifest in both strains at 48 h, and were usually more pronounced in the DBA/2J mice. In this strain, relative granulocyte and lymphocyte counts and the granulocyte/lymphocyte ratio correlated with viral HA mRNA expression and weight loss. In C57BL/6J, hematological parameters did not correlate with weight loss, but HA mRNA expression correlated weakly with total leukocyte counts, granulocyte/lymphocyte ratio, relative and absolute granulocyte counts, and relative lymphocyte counts. Significant changes due to mock treatment were mild and were detected only in C57BL/6J.ConclusionThis study underscores the value of hematological parameters in monitoring disease evolution in the early phase of IAV infection, and likely other pathogens. The hematological response to infection may differ significantly among inbred mouse strains.

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Virus dose-dependent neutrophil and lymphocyte proportions in peripheral blood during influenza A infection of mice

Cited by 4 publications

References 4 publications

The influence of dual infection with herpes and influenza viruses on the differential blood cell count of mice

The influence of dual infection with herpes and influenza viruses on the differential blood cell count of mice

Simultaneous infection with gammaherpes and influenza viruses enhances the host immune defense

Hematological parameters in the early phase of influenza A virus infection in differentially susceptible inbred mouse strains

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