Virus Diseases of Vegetable and Melon Crops in the South of the Russian Far East

Tolkach, V. F.; Kakareka, N. N.; Volkov, Yu. G.; Kozlovskaya, Z. N.; Sapotskiy, M. V.; Pleshakova, T. I.; D'yakonov, K. P.; Shchelkanov, M. Yu.

doi:10.18470/1992-1098-2019-4-121-133

Cited by 4 publications

(1 citation statement)

References 10 publications

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“…To measure the titter of infectious viral particles within infected plants, it is proposed to employ the method of plant infection and count the mosaic (necrotic) spots developed on the leaves of indicator plants depending on the concentration of viral particles, the number of spots that appear will vary. Empirically, it was noted, for example, that the tobacco mosaic virus titer can be investigated using isolated leaves of Nicotiana glutinosa L., Datura stramonium L., and Phaseolus vulgaris L. cultivated in a wet chamber [ 26 ]. Leaves of P. vulgaris were also suggested for the detection of tobacco necrosis virus and the ring spotting of tobacco, alfalfa mosaics, and common and southern bean mosaics.…”

Section: Methods For Plant Viral Diagnosticmentioning

confidence: 99%

Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review

Mehetre

Leo

Singh³

et al. 2021

Viruses

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Plant viral diseases are the foremost threat to sustainable agriculture, leading to several billion dollars in losses every year. Many viruses infecting several crops have been described in the literature; however, new infectious viruses are emerging frequently through outbreaks. For the effective treatment and prevention of viral diseases, there is great demand for new techniques that can provide accurate identification on the causative agents. With the advancements in biochemical and molecular biology techniques, several diagnostic methods with improved sensitivity and specificity for the detection of prevalent and/or unknown plant viruses are being continuously developed. Currently, serological and nucleic acid methods are the most widely used for plant viral diagnosis. Nucleic acid-based techniques that amplify target DNA/RNA have been evolved with many variants. However, there is growing interest in developing techniques that can be based in real-time and thus facilitate in-field diagnosis. Next-generation sequencing (NGS)-based innovative methods have shown great potential to detect multiple viruses simultaneously; however, such techniques are in the preliminary stages in plant viral disease diagnostics. This review discusses the recent progress in the use of NGS-based techniques for the detection, diagnosis, and identification of plant viral diseases. New portable devices and technologies that could provide real-time analyses in a relatively short period of time are prime important for in-field diagnostics. Current development and application of such tools and techniques along with their potential limitations in plant virology are likewise discussed in detail.

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Section: Methods For Plant Viral Diagnosticmentioning

confidence: 99%

Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review

Mehetre

Leo

Singh³

et al. 2021

Viruses

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Cucumber mosaic virus among ornamental crops in the Russian Far East

Tolkach,

Volkov,

Kakareka

et al. 2024

Ûg Ross.: èkol. razvit.

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Aim. To summarise and analyse scientific data on Cucumber mosaic virus strains (CMV – Cucumber mosaic virus) (Martellivirales: Bromoviridae, Cucumovirus) isolated from ornamental plants in the Far East of the Russian Federation. Discussion. The paper describes the genome structure and tripartite organization of CMV virions. Strains of this virus isolated from ornamental cultures in the south of the Russian Far East are described: Garden balsam, Common snapdragon, Dahlias, Hybrid gladiolus, Hybrid delphinium, Cambria, Indian canna, Cattleya, Tiger lily, Garden petunia, Primula obconica, Moth orchids, Fatshedera from Lize Freres, Weeping fig, Common hollyhock, Purple coneflower. The physicochemical properties (i. e. point of thermal inactivation, period of preservation of infectivity at a temperature of 20 °C, maximum dilution of juice causing disease of healthy plants) and the symptoms of these strains on a wide range of species and varieties of indicator plants are systematised according to the Russian Collection of East Asian Viruses, functioning at the Laboratory of Virology of the Federal Scientific Center for East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences. Conclusions. The limiting factor in the development of floriculture is infectious diseases, among which viral infections (including CMV) are of the greatest importance, demonstrating a high strain diversity in the south of the Russian Far East. Thus, the study of the biological, physico‐chemical, immunochemical and molecular biological properties of CMV is an urgent task, as it opens up the possibility of studying isolates of this virus and classifying its strains taking into account individual characteristics and kinship relationships.

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Diversity of phytovirus strains in the South of the Russian Far East

Kakareka,

Volkov,

Tolkach

et al. 2024

Ûg Ross.: èkol. razvit.

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Aim: to systematise data on the strain diversity of phytoviruses isolated in the South of the Russian Far East based on the analysis of the funds of the Russian Collection of East Asian Viruses of the Laboratory of Virology of the Federal Research Centre for Terrestrial Biodiversity of the Far Eastern Branch, Russian Academy of Sciences.The paper considers strains belonging to representatives of the genera Carlavirus (Tymovirales: Betaflexiviridae) (potato virus M, potato virus S, Vicia pseudorobus necrotic mosaic virus, lily symptomless virus); Cucumovirus (Martellivirales: Bromoviridae) (tomato aspermy virus, cucumber mosaic virus); Nepovirus (Picornavirales: Secoviridae) (raspberry ringspot virus, tobacco ringspot virus, tomato ringspot virus, dayflower mosaic virus, Phryma asiatica mosaic virus, Capsicum annuum necrotic spot virus, foenugreek necrotic spot virus); Potexvirus (Tymovirales: Alphaflexiviridae) (potato aucuba mosaic virus, potato virus X, white clover mosaic virus, Plantago asiatica mosaic virus, hydrangea ringspot virus, lily virus X); Potyvirus (Patatavirales: Potyviridae) (Onion yellow dwarf virus, Trifolium hybridum yellow mosaic virus, bean yellow mosaic virus, potato virus A, potato virus Y, Trifolium repens mottle virus, Hippeastrum mosaic virus, mountain clover mosaic virus, soybean mosaic virus, Tradescantia albiflora mosaic virus, turnip mosaic virus, bean common mosaic virus, soybean weak mosaic virus, soybean chlorotic deformation virus); Tobamovirus (Martellivirales: Virgaviridae) (cucumber green mottle mosaic virus, tobacco mosaic virus, tomato mosaic virus).The level revealed of strain variety of phytoviruses in the South of the Russian Far East as a reflection of the high biodiversity in this region requires regular ecological and phytovirusological monitoring in both natural and agrobiocenoses.

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Virus Diseases of Vegetable and Melon Crops in the South of the Russian Far East

Cited by 4 publications

References 10 publications

Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review

Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review

Cucumber mosaic virus among ornamental crops in the Russian Far East

Diversity of phytovirus strains in the South of the Russian Far East

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