Virulotyping and Antimicrobial Resistance Typing ofSalmonella entericaSerovars Relevant to Human Health in Europe

Abstract

The combination of virulence gene and antimicrobial resistance gene typing using DNA arrays is a recently developed genomics-based approach to bacterial molecular epidemiology. We have now applied this technology to 523 Salmonella enterica subsp. enterica strains collected from various host sources and public health and veterinary institutes across nine European countries. The strain set included the five predominant Salmonella serovars isolated in Europe (Enteritidis, Typhimurium, Infantis, Virchow, and Hadar). Initially, these strains were screened for 10 potential virulence factors (avrA, ssaQ, mgtC, siiD, sopB, gipA, sodC1, sopE1, spvC, and bcfC) by polymerase chain reaction. The results indicated that only 14 profiles comprising these genes (virulotypes) were observed throughout Europe. Moreover, most of these virulotypes were restricted to only one (n = 9) or two (n = 4) serovars. The data also indicated that the virulotype did not vary significantly with host source or geographical location. Subsequently, a representative subset of 77 strains was investigated using a microarray designed to detect 102 virulence and 49 resistance determinants. The results confirmed and extended the previous observations using the virulo-polymerase chain reaction screen. Strains belonging to the same serovar grouped together, indicating that the broader virulence-associated gene complement corresponded with the serovar. There were, however, some differences in the virulence gene profiles between strains belonging to an individual serovar. This variation occurred primarily within those virulence genes that were prophage encoded, in fimbrial clusters or in the virulence plasmid. It seems likely that such changes enable Salmonella to adapt to different environmental conditions, which might be reflected in serovar-specific ecology. In this strain subset a number of resistance genes were detected and were serovar restricted to a varying degree. Once again the profiles of those genes encoding resistance were similar or the same for each serovar in all hosts and countries investigated.

Identification of diverse Salmonella Serotypes, Virulotypes, and Antimicrobial Resistance Phenotypes in Waterfowl From Chile

“…Escherichia coli ATCC 25922 was used as a control strain. Virulence-associated genes pefA, spvC, sirA, gipA, SEN1417, trhH, and prot6e, all of which have been variably detected in the S. Enteritidis serotype (Pan et al 2009, Huehn et al 2010, were used as target sequences for the PCRbased virulotyping. DNA extraction was performed by the High Pure PCR Template preparation Kit Ò (Roche, Mannheim, Germany) following the manufacturer's recommendations.…”

Antimicrobial and molecular analysis of Salmonella serovar Livingstone strains isolated from humans in Tunisia and Belgium

“…Currently, alternative molecular typing methods such as multilocus sequence typing (MLST) and virulotyping [1,[14][15] are used to characterize many Salmonella enterica serovars. Multilocus sequence typing [16] has been increasingly recognized as a method of choice for genotyping a number of bacterial pathogens [17][18], including Salmonella [1,[19][20][21], and has been used successfully in epidemiological studies and outbreak investigations [17,22].…”

“…Virulence genes have been screened using simple [25] or multiplex PCR [26] to determine the distribution of these genes among Salmonella enterica strains [25,27] and to genotype many Salmonella serovars [15,[28][29].…”

Infection of chickens with antimicrobial-resistantSalmonella entericaTyphimurium DT193 and monophasicSalmonellaTyphimurium-like variants: an emerging risk to the poultry industry?

“…These ''epidemic'' monophasic isolates have been found to lack fljB, a gene that encodes phase 2 flagella Huehn et al, 2010). Monophasic members of Salmonella predominantly belong to two main phage types, DT193 and DT104, and pigs are considered the predominant source Huehn et al, 2010;Wasyl & Hoszowski, 2012).…”