Virulence targeted inhibitory effect of linalool against the exclusive uropathogen<i>Proteus mirabilis</i>

Durgadevi, Ravindran; Ravi, Arumugam Veera; Alexpandi, Rajaiah; Swetha, Thirukannamangai Krishnan; Abirami, G.; Vishnu, S. C; Pandian, Shunmugiah Karutha

doi:10.1080/08927014.2019.1619704

Cited by 24 publications

(16 citation statements)

References 57 publications

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“…The reduction at 1087 and 1238 cm −1 related to the breakdown of bacterial membrane phospholipids in photocatalyzed V. harveyi . The reduced intensity at 1550 and 1645 cm −1 denotes the damage to the amide-I and amide-II bonding of proteins and peptides 54 . The perceptible decrease in the intensity around 2750–3050 cm −1 indicates the damage to the fatty acid contents in the cell membrane in the photocatalyzed cells 17 .…”

Section: Resultsmentioning

confidence: 99%

Metal sensing-carbon dots loaded TiO2-nanocomposite for photocatalytic bacterial deactivation and application in aquaculture

Alexpandi

Gopi

Durgadevi

et al. 2020

Sci Rep

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Nowadays, bioactive nanomaterials have been attracted the researcher’s enthusiasm in various fields. Herein, Diplocyclos palmatus leaf extract-derived green-fluorescence carbon dots (DP-CDs) were prepared using the hydrothermal method. Due to the strong fluorescence stability, the prepared DP-CDs were coated on filter-paper to make a fluorometric sensor-strip for Fe 3+ detection. After, a bandgap-narrowed DP-CDs/TiO 2 nanocomposite (DCTN) was prepared using the methanolic extract of D. palmatus. The prepared DCTN exhibited improved photocatalytic bacterial deactivation under sunlight irradiation. The DCTN-photocatalysis slaughtered V. harveyi cells by the production of reactive oxygen species, which prompting oxidative stress, damaging the cell membrane and cellular constituents. These results suggest the plausible mode of bactericidal action of DCTN-photocatalysis under sunlight. Further, the DCTN has shown potent anti-biofilm activity against V. harveyi , and thereby, DCTN extended the survival of V. harveyi -infected shrimps during the in vivo trial with Litopenaeus vannamei . Notably, this is the first report for the disinfection of V. harveyi -mediated acute-hepatopancreatic necrosis disease (AHPND) using nanocomposite. The reduced internal-colonization of V. harveyi on the hepatopancreas as well as the rescue action of the pathognomonic effect in the experimental animals demonstrated the anti-infection potential of DCTN against V. harveyi -mediated AHPND in aquaculture.

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Section: Resultsmentioning

confidence: 99%

Metal sensing-carbon dots loaded TiO2-nanocomposite for photocatalytic bacterial deactivation and application in aquaculture

Alexpandi

Gopi

Durgadevi

et al. 2020

Sci Rep

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“…The cell-free and cell-bound EPS were collected from morin treated and untreated C. albicans culture, as described by Durgadevi et al (2019). C. albicans cells were grown at 37 • C for 16 h in YEPD media supplemented with and without morin.…”

Section: Eps Extractionmentioning

confidence: 99%

Inhibitory Effect of Morin Against Candida albicans Pathogenicity and Virulence Factor Production: An in vitro and in vivo Approaches

et al. 2020

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Candida albicans is considered an exclusive etiologic agent of candidiasis, a very common fungal infection in human. The expression of virulence factors contributes highly to the pathogenicity of C. albicans. These factors include biofilm formation, yeast-to-hyphal transition, adhesins, aspartyl proteases, and phospholipases secretion. Moreover, resistance development is a critical issue for the therapeutic failure of antifungal agents against systemic candidiasis. To circumvent resistance development, the present study investigated the virulence targeted therapeutic activity of the phytobioactive compound morin against C. albicans. Morin is a natural compound commonly found in medicinal plants and widely used in the pharmaceutical and cosmetic products/industries. The present study explicated the significant inhibitory potential of morin against biofilm formation and other virulence factors' production, such as yeast-hyphal formation, phospholipase, and exopolymeric substances, in C. albicans. Further, qPCR analysis confirmed the downregulation of biofilm and virlence-related genes in C. albicans upon morin treatment, which is in correspondence with the in vitro bioassays. Further, the docking analysis revealed that morin shows strong affinity with Hwp-1 protein, which regulates the expression of biofilm and hyphal formation in C. albicans and, thereby, abolishes fungal pathogenicity. Moreover, the anti-infective potential of morin against C. albicans-associated systemic candidiasis is confirmed through an in vivo approach using biomedical model organism zebrafish (Danio rerio). The outcomes of the in vivo study demonstrate that the morin treatment effectively rescues animals from C. albicans infections and extends their survival rate by inhibiting the internal colonization of C. albicans. Histopathology analysis revealed extensive candidiasis-related pathognomonic changes in the gills, intestine, and kidney of animals infected with C. albicans, while no extensive abnormalities were observed in morintreated animals. The results evidenced that morin has the ability to protect against the pathognomonic effect and histopathological lesions caused by C. albicans infection in zebrafish. Thus, the present study suggests that the utilization of morin could act as a potent therapeutic medication for C. albicans instigated candidiasis.

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“…The effect of HMB on P. mirabilis urease production was determined by the procedure described Durgadevi et al (2019) with modification. Briefly, 1% of P. mirabilis culture was allowed to grow in 1 ml of urea broth [peptone (1.5 g/L), sodium chloride (5 g/L), monopotassium phosphate (2 g/L), and filter sterilized urea (20 g/L)] supplemented with and without HMB (12.5, 25, and 50 µg/ml) for 24 h at 37 • C. After incubation, 0.02% phenol red reagent (pH indicator) was added to CFCS of both HMBtreated and untreated samples.…”

Section: Urease Assaymentioning

confidence: 99%

“…Owing to urease activity, P. mirabilis produces crystalline biofilm in the presence of urinary components such as urea, chloride, sodium, potassium, and other dissolved ions, and organic and inorganic compounds. For this study, artificial urine (AU) media was prepared in accordance with the procedure described by Durgadevi et al (2019) with minor changes. Briefly, 0.05% calcium chloride, 0.07% magnesium chloride, 0.2% sodium disulfate, 0.5% sodium chloride, 0.002% sodium oxalate, 0.06% trisodium citrate, 0.1% ammonium chloride, 0.3% potassium dihydrogen orthophosphate, 0.2% potassium chloride, and 2.5% urea were dissolved in sterile distilled water.…”

Section: Crystalline Biofilm Productionmentioning

confidence: 99%

“…A maximum of 60 and 51% reduction was observed at MBIC of HMB treatment in P. mirabilis ATCC and CI, respectively (Figure 2A). In FT-IR analysis, the IR spectra of EPS samples extracted from the control and HMB-treated (MBIC) P. mirabilis culture exhibited indistinguishable peak positions at 1,500-1,700 and 1,300-900 cm −1 , which corresponded to the proteins and mixed region of carbohydrates and nucleic acids, respectively (Durgadevi et al, 2019). However, the absorbance intensities of these spectral regions were found to be decreased in the EPS of HMB-treated P. mirabilis samples than those of the EPS of untreated control P. mirabilis samples ( Figure 2C).…”

Section: Effect Of 2-hydroxy-4-methoxybenzaldehyde On Exopolysaccharimentioning

confidence: 99%

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Explication of the Potential of 2-Hydroxy-4-Methoxybenzaldehyde in Hampering Uropathogenic Proteus mirabilis Crystalline Biofilm and Virulence

et al. 2019

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Proteus mirabilis is an important etiological agent of catheter-associated urinary tract infections (CAUTIs) owing to its efficient crystalline biofilm formation and virulence enzyme production. Hence, the present study explicated the antibiofilm and antivirulence efficacies of 2-hydroxy-4-methoxybenzaldehyde (HMB) against P. mirabilis in a non-bactericidal manner. HMB showed concentration-dependent biofilm inhibition, which was also evinced in light, confocal, and scanning electron microscopic (SEM) analyses. The other virulence factors such as urease, hemolysin, siderophores, and extracellular polymeric substances production as well as swimming and swarming motility were also inhibited by HMB treatment. Further, HMB treatment effectively reduced the struvite/apatite production as well as crystalline biofilm formation by P. mirabilis. Furthermore, the results of gene expression analysis unveiled the ability of HMB to impair the expression level of virulence genes such as flhB, flhD, rsbA, speA, ureR, hpmA, and hpmB, which was found to be in correlation with the results of in vitro bioassays. Additionally, the cytotoxicity analysis divulged the innocuous characteristic of HMB against human embryonic kidney cells. Thus, the present study reports the potency of HMB to act as a promising therapeutic remedy for P. mirabilis-instigated CAUTIs.

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Virulence targeted inhibitory effect of linalool against the exclusive uropathogenProteus mirabilis

Cited by 24 publications

References 57 publications

Metal sensing-carbon dots loaded TiO2-nanocomposite for photocatalytic bacterial deactivation and application in aquaculture

Metal sensing-carbon dots loaded TiO2-nanocomposite for photocatalytic bacterial deactivation and application in aquaculture

Inhibitory Effect of Morin Against Candida albicans Pathogenicity and Virulence Factor Production: An in vitro and in vivo Approaches

Explication of the Potential of 2-Hydroxy-4-Methoxybenzaldehyde in Hampering Uropathogenic Proteus mirabilis Crystalline Biofilm and Virulence

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