Virulence Potential of Listeria monocytogenes Recovered from Ice cream and Aborted Women Samples in Sohag city, Egypt

Abstract

L isteria monocytogenes is a gram-positive bacterial pathogen which is considered as a ubiquitous in nature and can be isolated from the environmental sources, including water, soil, sewage, vegetables, and food (Soni et al., 2015). The main route of infection by L. monocytogenes is through ingestion of contaminated food, such as unpasteurized milk, milk products and raw unwashed vegetables (Rawool et al., 2016).L. monocytogenes causing several outbreaks after the consumption of milk and dairy products especially ice cream (Shamloo et al., 2019). Ice cream is considered one of the most dairy products that predominates interest of popu-

The impact of Clove and thyme essential oils on Listeria monocytogenes isolated from meat and poultry products

“…The extraction of DNA from the suspected isolates was conducted according QIAamp manufacture Qiagen. The specific gene 16S rRNA of L. monocytogenes was detected using PCR and primers sequences F: GGA CCG GGG CTA ATA CCG AAT GAT AA and R: TTC ATG TAG GCG AGT TGC AGC CTA according to Hassanien and Shaker (2021) with some modifications in the cycling conditions as 5 min at 94°C, 35 cycles for 50s at 94°C, 50 °C for 1 min, and 60s at 72 °C, and final extension at 72 °C for 10 min. 1.5% agarose gel electrophoresis and ethidium bromide were used for examination of PCR product, and then photographed by light transilluminator (Biometra, Germany).…”