Virulence Phenotyping and Molecular Characterization of a New Virulence Type of Pyrenophora tritici-repentis the Causal Agent of Tan Spot

Benslimane, Hamida

doi:10.5423/ppj.nt.07.2017.0150

Cited by 12 publications

(5 citation statements)

References 18 publications

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“…Lamari et al (1998) were the first to report race 5 in Algeria, and this race has since been reported in Canada (Strelkov et al, 2002), the United States of America (Ali et al,1999), Syria and Azerbaijan (Lamari et al, 2005b). In contrast, race 6 was found in Algeria and Morocco (Strelkov et al, 2002;Benslimane, 2018;Gamba et al, 2017), while races 7 and 8 were found only in the Middle East, Caucasus and Algeria, and Kazakhstan in 2018 and 2020 (Kokhmetova et al, 2020b;Benslimane, 2018, Ouaar et al, 2022. In 2021, in the North Caucasus region of Russia, races 1, 3 and 4 were identified (Kremneva et al, 2021).…”

Section: Discussionmentioning

confidence: 98%

Characterization of Pyrenophora tritici-repentis (tan spot of wheat) races in Kazakhstan

Kumarbayeva

Кохметова²,

Kovalenko³

et al. 2022

Phytopathol. Mediterr.

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Tan spot, caused by Pyrenophora tritici-repentis, is an economically important foliar disease of wheat in Kazakhstan. Population structure of the pathogen changes every year due to climate change. This study aimed to characterize the race structure of P. tritici-repentis isolates recovered from wheat in south and north Kazakhstan, and identify tan spot resistance in host genotypes based on disease phenotypes and molecular screening. Virulence profiles were determined within 40 isolates of the pathogen from wheat crops during the 2020 growing season. Seven races, (1, 3, 4, 5, 6, 7 and 8) were identified. A collection of 80 wheat accessions, including promising lines and cultivars from Kazakhstan and Russia, were evaluated for their reactions to races 1 and 5 of the pathogen, and to Ptr ToxA and Ptr ToxB, using greenhouse assessments and molecular markers diagnostic for the Tsn1 and Tsc2 genes. From a practical viewpoint, 18 wheat genotypes were insensitive to the two races and the two Ptr toxins. This resistant germplasm can be used in breeding programmes aiming to develop wheat varieties resistant to P. tritici-repentis.

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Section: Discussionmentioning

confidence: 98%

Characterization of Pyrenophora tritici-repentis (tan spot of wheat) races in Kazakhstan

Kumarbayeva

Кохметова²,

Kovalenko³

et al. 2022

Phytopathol. Mediterr.

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“…Ptr can be classified into different races based on the ability of the pathogen to secrete necrotrophic effectors and induce necrotic and/or chlorotic symptoms on specific wheat genotypes. Eight Ptr races have been described in the literature based on three known effectors (ToxA, ToxB, and ToxC), however, new races have recently been described (Abdullah et al, 2017; Ali et al, 2010; Benslimane, 2018; Kamel et al, 2019; Kariyawasam et al, 2021) pointing to as yet uncharacterized effectors/virulence factors. Although the secretome of in vitro‐grown Ptr cultures has been investigated in a previous study (Cao et al, 2009), here we report a comparative study of the secretomes of two different Ptr races (races 1 and 5) using SWATH MS‐based identification.…”

Section: Discussionmentioning

confidence: 99%

Profiling the Pyrenophora tritici‐repentis secretome: The Pf2 transcription factor regulates the secretion of the effector proteins ToxA and ToxB

See

Moffat

2023

Molecular Microbiology

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Plant fungal pathogens secrete an array of compounds to circumvent host defence responses and facilitate colonization. For example, necrotrophic pathogens of the order Pleosporales which includes some of the major wheat pathogens, produce effector proteins that trigger a specific host sensitivity response in an inverse geneto-gene manner (Friesen et al., 2007;Strelkov & Lamari, 2003).The interaction is governed by a complex and specific recognition between the host and the pathogenicity factors, as demonstrated by two of the most well-studied effector proteins, ToxA (Ballance

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“…This may well provide an explanation for the observed distinction of Ls13-192 from the other race 4 isolates (90-2 and SD20) in the whole genome phylogenetic clustering and gene correlation analyses, since unlike Ls13-192, SD20 and 90-2 have not been reported to be virulent on durum wheat. Furthermore, as ToxA and ToxB were absent, race 4 isolate T205 is unlike the new virulence type that lacked ToxA and ToxB gene expression on bread wheat differentials but produced necrosis in durum wheat [ 85 ].…”

Section: Discussionmentioning

confidence: 99%

A global pangenome for the wheat fungal pathogen Pyrenophora tritici-repentis and prediction of effector protein structural homology

et al. 2022

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The adaptive potential of plant fungal pathogens is largely governed by the gene content of a species, consisting of core and accessory genes across the pathogen isolate repertoire. To approximate the complete gene repertoire of a globally significant crop fungal pathogen, a pan genomic analysis was undertaken for Pyrenophora tritici-repentis (Ptr), the causal agent of tan (or yellow) spot disease in wheat. In this study, 15 new Ptr genomes were sequenced, assembled and annotated, including isolates from three races not previously sequenced. Together with 11 previously published Ptr genomes, a pangenome for 26 Ptr isolates from Australia, Europe, North Africa and America, representing nearly all known races, revealed a conserved core-gene content of 57 % and presents a new Ptr resource for searching natural homologues (orthologues not acquired by horizontal transfer from another species) using remote protein structural homology. Here, we identify for the first time a non-synonymous mutation in the Ptr necrotrophic effector gene ToxB, multiple copies of the inactive toxb within an isolate, a distant natural Pyrenophora homologue of a known Parastagonopora nodorum necrotrophic effector (SnTox3), and clear genomic break points for the ToxA effector horizontal transfer region. This comprehensive genomic analysis of Ptr races includes nine isolates sequenced via long read technologies. Accordingly, these resources provide a more complete representation of the species, and serve as a resource to monitor variations potentially involved in pathogenicity.

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Virulence Phenotyping and Molecular Characterization of a New Virulence Type of Pyrenophora tritici-repentis the Causal Agent of Tan Spot

Cited by 12 publications

References 18 publications

Characterization of Pyrenophora tritici-repentis (tan spot of wheat) races in Kazakhstan

Characterization of Pyrenophora tritici-repentis (tan spot of wheat) races in Kazakhstan

Profiling the Pyrenophora tritici‐repentis secretome: The Pf2 transcription factor regulates the secretion of the effector proteins ToxA and ToxB

A global pangenome for the wheat fungal pathogen Pyrenophora tritici-repentis and prediction of effector protein structural homology

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