Virulence of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) Strain ESC-1 to the German Cockroach (Dictyoptera: Blattellidae) and Its Compatibility with Insecticides

Pachamuthu, Pari; Kamble, Shripat T.; Yuen, Gary Y.

doi:10.1093/jee/92.2.340

Cited by 25 publications

(30 citation statements)

References 22 publications

(35 reference statements)

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“…The entomopathogenic fungus Metarhizium anisopliae (Metschnikoff) Sorokin has been isolated from 200 insects species including the orders Lepidoptera, Coleoptera, Orthoptera, and Hemiptera (Charnley 1989, Zimmermann 1993. There are few reports on the use of this fungus for urban pest management (Gunner et al 1991;Kelley-Tunis et al 1995;Kaakeh et al 1996Kaakeh et al , 1997Pachamuthu et al 1999). According to Gunner et al (1991), M. anisopliae strain PA-2 required Ϸ6 wk to produce 90% mortality in German cockroaches, whereas Kaakeh et al (1996) reported 26 Ð30 d to achieve 90% or higher mortality with M. anisopliae strain ESC-1 in controlling German cockroaches by contact method.…”

mentioning

confidence: 99%

In Vivo Study on Combined Toxicity of <I>Metarhizium anisopliae</I> (Deuteromycotina: Hyphomycetes) Strain ESC-1 with Sublethal Doses of Chlorpyrifos, Propetamphos, and Cyfluthrin Against German Cockroach (Dictyoptera: Blattellidae)

Pachamuthu

Kamble

2000

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mentioning

confidence: 99%

In Vivo Study on Combined Toxicity of <I>Metarhizium anisopliae</I> (Deuteromycotina: Hyphomycetes) Strain ESC-1 with Sublethal Doses of Chlorpyrifos, Propetamphos, and Cyfluthrin Against German Cockroach (Dictyoptera: Blattellidae)

Pachamuthu

Kamble

2000

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“…A comparação de tais resultados com os de outros trabalhos na literatura demonstra a grande variação existente entre os isolados, haja vista que Pachamuthu et al (1999), Oliveira et al (2003) e Andaló et al (2004), obtiveram entre 4,9 × 10 7 a 1,6 × 10 9 conídios/colônia, provavelmente em decorrência da variabilidade genética entre os isolados.…”

Section: Resultsunclassified

Efeito da temperatura e cama do aviário na virulência de Beauveria bassiana (Bals.) Vuill. e Metarhizium anisopliae (Metsch.) para o controle do cascudinho (Alphitobius diaperinus) (Panzer) (Coleoptera: Tenebrionidae)

Alexandre¹,

Alves²,

Neves³

et al. 2006

Neotrop. Entomol.

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Bolsista de Produtividade em Pesquisa/CNPqNeotropical Entomology 35(1): 075-082 (2006) Effect of Temperature and Poultry Litter in Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch) Virulence against the Lesser Mealworm Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)ABSTRACT -This study was carried out to evaluate the influence of temperature and poultry litter on germination vegetative growth virulence and conidial production of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) isolates on larvae and adults of the lesser mealworm (Alphitobius diaperinus) (Panzer). The vegetative growth and conidial production were evaluated on culture media. Virulence was studied submerging larvae and adults in a conidial suspension (1 x 10 8 conidia/ml). All the experiments were carried out in growth chamber (26ºC and 32ºC and 14h photophase). Fungus-killed insects were daily collected and used for microscopic conidial counts. The poultry litter effect was evaluated by submerging the insects in a fungal suspension (10 8 conidia/ml) and then transferring them to cups containing poultry litter (new and used). B. bassiana isolates were more sensitive than M. anisopliae to high temperature because conidia viability, vegetative growth and virulence were negatively affected (P < 0,05). The conidial production was higher to B. bassiana in 26ºC (7 to 11 x 10 8 conidia/larval cadaver and 8 x 10 8 conidia/adult cadaver) (P < 0,05). Larval stage was about 10 times more sensitive to M. anisopliae at 26ºC than adults stage. Regarding B. bassiana, differences on sensitivity between larval stages and adults were not observed at this temperature. However, at 32ºC, larval stage was more sensitive for CB116 and UEL50 isolates. Mortality was higher when larvae and adults (15.7 and 66.7% respectively) were treated by B. bassiana and maintained on new poultry litter at 26ºC) (P < 0,05).KEY WORDS: Entomopathogenic fungus, microbial control, animal production RESUMO -O estudo avaliou a influência da temperatura e de cama nova e usada na germinação, crescimento vegetativo, virulência e produção de conídios de isolados de Beauveria bassiana (Bals.) Vuill. e Metarhizium anisopliae (Metsch.) sobre larvas e adultos do cascudinho Alphitobius diaperinus (Panzer). O crescimento vegetativo e a produção de conídios foram avaliados em meio de cultura. Para a avaliação da virulência os insetos foram tratados com suspensões contendo 1 x 10 8 conídios/ ml. Os experimentos foram realizados em câmara B.O.D. (26ºC e 32ºC e 14h de fotofase). Diariamente os insetos mortos foram coletados para quantificação da produção de conídios. Para avaliação das camas, após a inoculação, insetos foram transferidos para recipientes contendo cama de aviário (nova e usada). Os isolados de B. bassiana foram mais sensíveis à temperatura elevada em relação aos isolados de M. anisopliae no que se refere à viabilidade, crescimento vegetativo e virulência (P < 0,05). A conidiogênese também foi maior para B. bassiana a 26ºC (7 a 11 x 10...

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“…Most recently, the compatibility (increased mortality and lower LT 50 ) of M. anisopliae was demonstrated with imidacloprid (Kaakeh et al, 1997), chlorpyrifos, prometamphos, and cyfluthrin (Pachamutu et al, 1999;Pachamutu and Kamble, 2000) against the German cockroach. Boric acid also enhanced the virulence of Bacillus thuringiensis (Berliner) subsp.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…However, its slow action (usually ϳ30 days) and inconsistent efficacy have confounded effective use of this pathogen under field conditions, including the structural environment (Pachamutu et al, 1999). For these reasons, sales of a commercial formulation of M. anisopliae for cockroaches, Bio-Path by EcoScience Corp. (Worcester, MA), were discontinued (Pachamutu et al, 1999). Recently, several studies reported increased virulence of M. anisopliae against B. germanica in combination with the organic insecticides imidacloprid, chlorpyrifos, propetamphos, and cyfluthrin (Kaakeh et al, 1997;Pachamutu et al, 1999;Pachamutu and Kamble, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Synergism between Metarhizium anisopliae (Deuteromycota: Hyphomycetes) and Boric Acid against the German Cockroach (Dictyoptera: Blattellidae)

2002

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Virulence of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) Strain ESC-1 to the German Cockroach (Dictyoptera: Blattellidae) and Its Compatibility with Insecticides

Cited by 25 publications

References 22 publications

In Vivo Study on Combined Toxicity of <I>Metarhizium anisopliae</I> (Deuteromycotina: Hyphomycetes) Strain ESC-1 with Sublethal Doses of Chlorpyrifos, Propetamphos, and Cyfluthrin Against German Cockroach (Dictyoptera: Blattellidae)

In Vivo Study on Combined Toxicity of <I>Metarhizium anisopliae</I> (Deuteromycotina: Hyphomycetes) Strain ESC-1 with Sublethal Doses of Chlorpyrifos, Propetamphos, and Cyfluthrin Against German Cockroach (Dictyoptera: Blattellidae)

Efeito da temperatura e cama do aviário na virulência de Beauveria bassiana (Bals.) Vuill. e Metarhizium anisopliae (Metsch.) para o controle do cascudinho (Alphitobius diaperinus) (Panzer) (Coleoptera: Tenebrionidae)

Synergism between Metarhizium anisopliae (Deuteromycota: Hyphomycetes) and Boric Acid against the German Cockroach (Dictyoptera: Blattellidae)

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