Virulence of Escherichia coli in relation to host factors in women with symptomatic urinary tract infection

Sandberg, Torsten; Kaijser, B; Lidin-Janson, G; Lincoln, K.; Ørskov, Frits; Ørskov, Ida; Stokland, Eira; Svanborg-Edén, C

doi:10.1128/jcm.26.8.1471-1476.1988

Cited by 85 publications

(27 citation statements)

References 34 publications

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“…Epidemiological studies have shown that many E. coli Kl strains isolated from different sources and geographic locations belong to a small number of genetic clones (2,3,37,51). Some of these genetic clones were associated with particular virulence in human urinary tract infection and others were associated with neonatal bacteremia and meningitis (3,32,47). Studies with laboratory animals have shown that the clonal type is not always in conformity with the virulence of strains and virulence markers of strains belonging to the same clonal type are often diverse (1,3,37,45,51; also this study).…”

Section: Discussionsupporting

confidence: 55%

“…Escherichia coli carrying the Kl capsule is a commonly occurring bacteria in the feces of healthy humans and is an important pathogen that causes bacteremia and meningitis in newborn infants (23,34). In addition, some types of E. coli Kl strains were found to be of particular virulence in pyelonephritis in humans of all age groups (23,32,47). The somatic antigens of E. coli Kl are associated with their virulence.…”

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confidence: 99%

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Influence of some bacterial and host factors on colonization and invasiveness of Escherichia coli K1 in neonatal rats

et al. 1993

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Of 209 healthy infants examined, 44 (21.1%) carried Escherichia coli Kl in their feces. Of these 44 isolates, 36 (81.8%) were attributed to 10 different known clonal groups of E. coli Kl and 4 isolates represented unknown types. The influence of mannose-resistant (MR) adhesins, aerobactin production, and resistance to serum on colonization and invasiveness of E. coli KJ in orally infected inbred LEW baby rats was investigated.Strains expressing MR adhesins had significantly higher colonization and invasion rates than non-MR strains did. Mixed-infection experiments of LEW rats revealed interactions between different types of E. coli Kl strains affecting colonization and invasion rates. P-fimbriated strains appeared to have a selective advantage for colonization. The bacteremic potentials of different E. coli Kl strains could not be associated with their resistance to sera from LEW rats free of members of the family Enterobacteriaceae. No differences in virulence between fecal E. coli Kl isolates and clinical isolates from diseased humans were found. An influence of the major histocompatibility complex on host susceptibility to invasive E. coli Kl was indicated by comparing the parental LEW rat strain with different congenic LEW strains (RTI).

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Section: Discussionsupporting

confidence: 55%

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confidence: 99%

Influence of some bacterial and host factors on colonization and invasiveness of Escherichia coli K1 in neonatal rats

et al. 1993

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“…The biological characteristics of uropathogenic E. coli strains (UPEC) include hemolysin and aerobactin production, expression of P fimbriae, serum resistance, cytotoxic necrotizing factor (CNF), and capsule production. These strains belong to a small number of O serogroups 10,13,14,17,24,25,27,32,40 . The genes responsible for expression of these characteristics are normally clustered in DNA regions denominated pathogenic islands (PAIs) 9,20,37 .…”

Section: Introductionmentioning

confidence: 99%

Biological and genetic characteristics of uropathogenic Escherichia coli strains

Silveira

Benetti

Lancellotti

et al. 2001

Rev. Inst. Med. trop. S. Paulo

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The aim of the present study was to determine biological characteristics such as expression of fimbriae, Congo red binding, production of hemolysin and aerobactin, adhesion to HeLa and uroepithelial cells and invasion of HeLa cells by Escherichia coli isolates obtained from patients showing clinical signs of urinary tract infection (UTI). Also, the presence of genes (apa, afa, spa) for fimbria expression and cytotoxic necrotizing factors (CNF1, CNF2) was assayed using specific primers in PCR. The data obtained were compared with the clonal relationships obtained by analysis of multilocus enzyme electrophoresis (MLEE), restriction fragment length polymorphism (RFLP) of the rDNA (ribotyping) and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR). All isolates but one presented a combination of at least two of the characteristics studied, a fact suggesting the presence of pathogenicity islands (PAIs). Diffuse adherence type to HeLa cells was observed to occur in most of the strains, but adhesion to uroepithelial cells seems to be a more reliable test to verify pathogenicity. Although four strains seemed to be able to invade HeLa cells when assayed by light microscopy, electron microscopy studies demonstrated that these strains were not invasive. MLEE, RFLP and ERIC-PCR were able to group the isolates differently into main clusters that were not correlated with the presence of pathogenic traits.

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“…A reasonable question might be whether the only distinction between cystitis and pyelonephritis is the site where the organism happens to attach. There are reports suggesting that this sometimes may be the case, i.e., that apparently the same organism earlier causing acute pyelonephritis has caused cystitis upon recurrence in the same patient (2,33). However, there are other data indicating that cystitis strains as a group are different from pyelonephritis strains.…”

Section: Discussionmentioning

confidence: 99%

Comparison ofEscherichia coliStrains Recovered from Human Cystitis and Pyelonephritis Infections in Transurethrally Challenged Mice

Johnson

Lockatell

Russell³

et al. 1998

Infect Immun

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Urinary tract infection, most frequently caused byEscherichia coli, is one of the most common bacterial infections in humans. A vast amount of literature regarding the mechanisms through which E. coli induces pyelonephritis has accumulated. Although cystitis accounts for 95% of visits to physicians for symptoms of urinary tract infections, few in vivo studies have investigated possible differences between E. coli recovered from patients with clinical symptoms of cystitis and that from patients with symptoms of pyelonephritis. Epidemiological studies indicate that cystitis-associated strains appear to differ from pyelonephritis-associated strains in elaboration of some putative virulence factors. With transurethrally challenged mice we studied possible differences using three each of the most virulent pyelonephritis and cystitis E. coli strains in our collection. The results indicate that cystitis strains colonize the bladder more rapidly than do pyelonephritis strains, while the rates of kidney colonization are similar. Cystitis strains colonize the bladder in higher numbers, induce more pronounced histologic changes in the bladder, and are more rapidly eliminated from the mouse urinary tract than pyelonephritis strains. These results provide evidence that cystitis strains differ from pyelonephritis strains in this model, that this model is useful for the study of the uropathogenicity of cystitis strains, and that it would be unwise to use pyelonephritis strains to study putative virulence factors important in the development of cystitis.

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Virulence of Escherichia coli in relation to host factors in women with symptomatic urinary tract infection

Cited by 85 publications

References 34 publications

Influence of some bacterial and host factors on colonization and invasiveness of Escherichia coli K1 in neonatal rats

Influence of some bacterial and host factors on colonization and invasiveness of Escherichia coli K1 in neonatal rats

Biological and genetic characteristics of uropathogenic Escherichia coli strains

Comparison ofEscherichia coliStrains Recovered from Human Cystitis and Pyelonephritis Infections in Transurethrally Challenged Mice

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