Virulence of Escherichia coli in acute pyelonephritis, acute cystitis and asymptomatic bacteriuria

Bosch, J. F. van den; Oe, P. L.; Postma, P.; Graaff, J. de; Maclaren, D. M.; Jansen, Wim; Guiñée, P. A. M.

doi:10.1007/bf00421979

Cited by 8 publications

(13 citation statements)

References 21 publications

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“…None of these differences is so pronounced that it alone could account for this higher prevalence of P. mirabilis. Studies such as those of Brooks et al (1980) and of Van den Bosch et al (1980) have already shown that virulence in these commensal pathogens is a multifactorial phenomenon. Judgement of the contribution of each separate factor to the bacterial virulence is therefore difficult and requires further studies.…”

Section: Discussionmentioning

confidence: 99%

Uropathogenic Properties of Proteus Mirabilis and Proteus Vulgaris

Peerbooms

Verweij

Maclaren

1985

Journal of Medical Microbiology

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SUMMARY. A group of faecal isolates of Proteus vulgaris and P. mirabilis was studied for the presence of possible virulence factors such as growth rates in urine and broth, haemolysin production, hydrophobicity, sensitivity to the bactericidal activity of human serum and cell invasiveness. Differences were found in haemolysin production, cell invasiveness and experimental virulence in a mouse model. These differences might explain why P. mirabilis is much more common in human urinary-tract infections than P. vulgaris. INTRODUCTIONProteus vulgaris and P. mirabilis are common inhabitants of the human gut; Krikler (1953) reported prevalences of 17% for P. mirabilis and 5% for P. vulgaris in the faeces of healthy persons. Urinary pathogens are thought to originate mainly from the gut and it is interesting that P. mirabilis is disproportionately more frequently isolated from patients with urinary-tract infections than P. vulgaris. Senior (1 979), for example, isolated 258 strains of P. mirabilis from infected urines against only four strains of P. vulgaris, two of which were isolated together with P. mirabilis. These epidemiological data suggest that P. mirabilis is more likely to cause urinary-tract infections than P. vulgaris. As both species are very similar, genetically and biochemically (Brenner et al., 1978), a comparative study of isolates of both species could yield valuable information about bacterial properties important in the pathogenesis of urinary-tract infections. Therefore, we have compared faecal isolates of both species, isolated from the same population, with regard to several properties: experimental virulence in a mouse model, growth rates in urine and broth, haemolysin production, hydrophobicity, sensitivity to the bactericidal activity of human serum, cell invasiveness and serotype. MATERIALS AND METHODSIsolation of Proteus strains from faeces. Stool samples were obtained from patients seen in general practice. Most samples had been submitted for examination for occult blood or for suspected parasitic infestation. A suspension of the sample in phosphate-buffered saline (PBS) was inoculated in the centre of a fresh nutrient-agar plate (Oxoid). The plates were incubated for ~ ~

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Section: Discussionmentioning

confidence: 99%

Uropathogenic Properties of Proteus Mirabilis and Proteus Vulgaris

Peerbooms

Verweij

Maclaren

1985

Journal of Medical Microbiology

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“…The 55 strains of E. coli used in this study were isolated from cases of urinary-tract infection either in general practice or in hospital patients, and were described previously (van den Bosch et al, 1979, 1980a and. Strains AD27 and AD27b were kindly supplied by Dr L. Emody (University of PCcs, Hungary), and were originally numbered P673 and P673/ 1.…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study we found differences in virulence between different strains of E. coli in a mouse model (van den Bosch, de Graaff and MacLaren, 1979). We were able to divide the strains into three virulence groups on the basis of the kinetics of the viable counts in the kidney and other organs after intravenous injection, and the measurement of LD50 values and killing times.…”

Section: Introductionmentioning

confidence: 93%

Haemolysis by urinary Escherichia coli and virulence in mice

Bosch

Postma

Graaff

et al. 1981

Journal of Medical Microbiology

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PLATE XVIIISUMMARY. The influence of haemolysin production on virulence was studied in an experimental mouse model. Urinary strains of Escherichia coli can be divided into three virulence groups by determining their kinetics in the mouse kidney after intravenous injection. Virulent strains of groups I1 and I11 were more often haemolytic than avirulent group-I strains. Haemolytic virulent strains often caused haemoglobinuria in the mice, and killed the mice more rapidly than did non-haemolytic virulent strains. No relationship was found between a-haemolytic activity and virulence in wild-type haemolytic strains. When haemolysin production was reduced or eliminated by treatment with actinomycin-D or rifampicin, six out of seven group-I1 strains tested gave the same results as avirulent group-I strains. However, the kinetics in the mouse kidney of four haemolytic group-I11 strains tested was not changed after reduction or elimination of haemolysin production; only a small decrease in toxicity was observed. It is concluded that haemolysin production by E. coli is a decisive virulence factor in most of the mouse-nephropathogenic group-I1 strains, but not in the virulent group-I11 strains.

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“…A small group with a pattern intermediate between groups II and III was named group IV. Strains isolated from acute pyelonephritis were more often virulent in the mouse model than strains from acute cystitis and from asymptomatic bacteriuria (ABU) (van den Bosch et al 1980a), whereas cystitis strains were not more often virulent than normal faeces strains (van den Bosch et al 1981 a). Therefore we have suggested that E. coli strains reach the bladder in general in proportion to their frequency in the faecal flora and subsequently may invade the kidney and cause serious infection in relation to their virulence.…”

Section: Introductionmentioning

confidence: 97%

Virulence of urinary and faecalEscherichia coliin relation to serotype, haemolysis and haemagglutination

Bosch

Postma

Koopman

et al. 1982

J. Hyg.

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SUMMARYThe virulence offaecal and urinary Escherichia coli strains was studied in relation to serotype, haemolysin production and haemagglutination pattern. By means of an experimental mouse model E. coli strains can be divided into avirulent (I), mouse nephropathogenic (II), and generally virulent (III) strains. Virulent group II and group III strains were more often haemolytic and haemagglutinating than avirulent group I strains. Presence of K antigen could not be associated with virulence. Discriminant analysis for qualitative variables revealed that no combination of the investigated properties contributed more to a strain's virulence level than did one single property. It is concluded that other virulence factors, apart from haemolysin production in group II strains and haemagglutinins in group III strains, must be involved in the determination of a strain's virulence level.All 02, 06 and 018 ac strains tested were virulent, and by far the most 075 strains were avirulent, whereas other 0 groups were more variable with regard to virulence. Pyelonephritis strains were more often mannose-resistant haemagglutinating than faecal and other urinary isolates, indicating that mannoseresistant adhesins may be important in the pathogenesis of pyelonephritis.

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Virulence of Escherichia coli in acute pyelonephritis, acute cystitis and asymptomatic bacteriuria

Cited by 8 publications

References 21 publications

Uropathogenic Properties of Proteus Mirabilis and Proteus Vulgaris

Uropathogenic Properties of Proteus Mirabilis and Proteus Vulgaris

Haemolysis by urinary Escherichia coli and virulence in mice

Virulence of urinary and faecalEscherichia coliin relation to serotype, haemolysis and haemagglutination

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