Virulence of Cryptococcus neoformans serotypes A, B, C and D for four mouse strains

Irokanulo, E. A. O.; Akueshi, C. O.

doi:10.1099/00222615-43-4-289

Cited by 16 publications

(8 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One must be careful though not to generalize data obtained with this strain. Differences in virulence have been noted within and between serotypes of clinical strains of C. neoformans, even using the same animal model (3,16). Clearly, ideal vaccine candidates should protect against the vast majority of isolates likely to be encountered clinically.…”

Section: Discussionmentioning

confidence: 99%

Protective Efficacy of Antigenic Fractions in Mouse Models of Cryptococcosis

et al. 2004

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Protective Efficacy of Antigenic Fractions in Mouse Models of Cryptococcosis

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Mice are less expensive than larger laboratory mammals, and their genetics, specific immunological responses, and innate immunity are well defined. However, inbred strains of mice vary in their susceptibility (or resistance) to C. neoformans (4,22,33,37,41). Experimental cryptococcosis in rats is more subacute and reminiscent of human disease (16).…”

Section: Discussionmentioning

confidence: 99%

Most Environmental Isolates ofCryptococcus neoformansvar.grubii(Serotype A) Are Not Lethal for Mice

Litvintseva

Mitchell

2009

Infect Immun

View full text Add to dashboard Cite

Most cases of cryptococcosis are caused by Cryptococcus neoformans var. grubii (serotype A), which is widespread in the environment, where it is primarily associated with pigeon excreta. A number of molecular epidemiological studies indicate that many environmental and clinical isolates of serotype A are indistinguishable. However, the murine virulence of environmental strains of C. neoformans has not been thoroughly evaluated. We used the murine intranasal model of cryptococcosis to compare the lethality of clinical and environmental strains of serotype A that possessed identical genotypes as determined by amplified fragment length polymorphisms (AFLP) and multilocus sequence typing (MLST). Eleven environmental strains were tested, and only one caused disease within 60 days postinfection, at which time the experiments were terminated. Conversely, 7 of 10 clinical isolates were lethal for mice at median times of 19 to 40 days. Passing environmental isolates in mice (up to three times) did not significantly increase their lethality. In follow-up studies, we developed a new genotyping technique based on hybridization with TCN2 and TCN4 retrotransposon-specific probes. Although the retrotransposon banding patterns were unstable after prolonged incubation in the laboratory, this method was able to differentiate clinical and environmental strains that had the same AFLP/MLST genotypes.

show abstract

“…AD hybrids showed hybrid vigor (heterosis), adapting to changing environments better than either A or D isolates . Furthermore, extensive LOH in AD hybrid strains resulted in preferential retention of the serotype A homolog of Chr1 (Hu et al 2008), which may contribute to a selective advantage in the mammalian host (Kwon-Chung and Bennett 1984;Irokanulo and Akueshi 1995;Lin et al 2008). This provides an example of how LOH has the potential to confer adaptive benefits under selective pressure.…”

Section: Loss Of Heterozygositymentioning

confidence: 99%