Virulence markers associated with <i>Trueperella pyogenes</i>
 infections in livestock and companion animals

Risseti, Rafaela Mastrangelo; Zastempowska, Ewa; Twarużek, Magdalena; Lassa, H.; Pantoja, J.C.F.; Vargas, Águeda Castagna de; Guerra, Simony Trevizan; Bolaños, Carmen Alicia Daza; Paula, Carolina Lechinski de; Alves, Ana Carolina; Colhado, Bruna S.; Portilho, Fábio Vinícius Ramos; Tasca, Caiane; Lara, Gustavo Henrique Batista; Ribeiro, Márcio Garcia

doi:10.1111/lam.12757

Cited by 28 publications

(34 citation statements)

References 14 publications

(61 reference statements)

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“…3,6,16 Interestingly, the lack of cbpA among caprine and ovine isolates in our study is consistent with findings of prior studies in which all tested isolates from goats and sheep tested negative for cbpA. 6,12,14 In addition to CbpA, T. pyogenes isolates can express neuraminidases H (NanH) and P (NanP), molecules that are also involved in bacterial adhesion. 8 The occurrence of genes encoding NanH and NanP have been shown to vary 40% and 64%, respectively, to 100% in bovine T. pyogenes isolates.…”

Section: Discussionsupporting

confidence: 91%

“…Studies involving virulence factors are important for investigation of molecular epidemiology and pathogenicity of various pathogens. 12 Our study contributes to the understanding of phenotypic and genotypic diversity that exist among T. pyogenes isolates recovered from large and small ruminants and, overall, reinforces the importance of characterization of more clinical isolates in order to better understand the pathogenesis of this bacterium in different animal species.…”

Section: Discussionsupporting

confidence: 68%

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Phenotypic and genotypic characteristics of Trueperella pyogenes isolated from ruminants

et al. 2018

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Trueperella pyogenes is an opportunistic pathogen that causes suppurative infections in animals including humans. Data on phenotypic and genotypic properties of T. pyogenes isolated from ruminants, particularly goats and sheep, are lacking. We characterized, by phenotypic and genotypic means, T. pyogenes of caprine and ovine origin, and established their phylogenetic relationship with isolates from other ruminants. T. pyogenes isolates ( n = 50) from diagnostic specimens of bovine ( n = 25), caprine ( n = 19), and ovine ( n = 6) origin were analyzed. Overall, variable biochemical activities were observed among the T. pyogenes isolates. The fimbriae-encoding gene, fimE, and neuraminidase-encoding gene, nanH, were, respectively, more frequently detected in the large ( p = 0.0006) and small ( p = 0.0001) ruminant isolates. Moreover, genotype V ( plo/ nanH/ nanP/ fimA/ fimC) was only detected in the caprine and ovine isolates, whereas genotype IX ( plo/ nanP/ fimA/ fimC/ fimE) was solely present in the isolates of bovine origin ( p = 0.0223). The 16S rRNA gene sequences of all T. pyogenes isolates were clustered with the reference T. pyogenes strain ATCC 19411 and displayed a high degree of identity to each other. Our results highlight phenotypic and genotypic diversity among ruminant isolates of T. pyogenes and reinforce the importance of characterization of more clinical isolates to better understand the pathogenesis of this bacterium in different animal species.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 68%

Phenotypic and genotypic characteristics of Trueperella pyogenes isolated from ruminants

et al. 2018

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“…In Brazil, in the state of Minas Gerais, isolation and identification were carried out of M. haemolytica bacteria as a causative agent of pneumonia in ovines 80 . Species of the genus Trueperella are known to be important opportunistic pathogens in livestock and domestic animals, causing a variety of infections 81,82 , as well as mastitis and uterine infections in cows 83,84 . Mastitis caused by T. pyogenes is considered of minor importance in goats 22,85 .…”

Section: Discussionmentioning

confidence: 99%

Effects of enrofloxacin treatment on the bacterial microbiota of milk from goats with persistent mastitis

Polveiro

Vidigal

Mendes

et al. 2020

Sci Rep

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Antibiotic resistance has become a major concern for human and animal health. As fluoroquinolones have been extensively used in human and veterinary medicine, there has also been the rapid emergence and spread of antimicrobial resistance around the world. Here, we analysed the microbiome of goat milk using samples from healthy goats and those diagnosed with persistent mastitis and treated using the antibiotic enrofloxacin with 16S rRNA amplicon sequencing. We selected a group of 11 goats and 22 samples of milk that did not respond clinically to enrofloxacin treatment. Milk samples were evaluated before and after treatment to verify changes of the microbiota; the three first lactating goats were selected from the healthy control group. The milk samples from the healthy control animals presented a larger abundance of different species of bacteria of the Staphylococcus genus, but a smaller number of different genera, which indicated a more specific niche of resident bacteria. The Firmicutes phylum was predominantly different between the studied groups. Samples from before-treatment animals had a higher number of new species than those from the control group, and after being treated again. These microbiota received new bacteria, increasing the differences in bacteria even more in relation to the control group. Genotypes such as Trueperella and Mannheimia, between other genera, had a high abundance in the samples from animals with persistent mastitis. The dysbiosis in this study, with marked evidence of a complex microbiota in activity in cases of the failure of antimicrobial treatment for persistent chronic mastitis, demonstrates a need to improve the accuracy of pathogen identification and increases concern regarding antibiotic treatments in milk production herds. Antimicrobial therapies have been shown to be increasingly problematic due to the development multiple types of antimicrobial resistance (AMR) mechanisms, and for that reason, therapeutic alternatives to treat multidrug-resistant microorganisms are rapidly dwindling. Fluoroquinolones have been extensively used in human and veterinary medicine as they are considered among the most effective drugs for the treatment of bacterial infections 1,2. Enrofloxacin, is a fluoroquinolone exclusively developed for use in veterinary medicine 1,3,4. This drug is a potent inhibitor of bacterial DNA Topoisomerase II (Gyrase) and the DNA Topoisomerase IV (Topo IV), which are essential enzymes involved in key cellular processes including DNA replication 5-10. The drug has a broad spectrum of activity, being active against major pathogenic bacteria (both Gram-positive and Gram-negative), mycoplasmas 11 , and also mycobacteria 12 , but is ineffective against obligate anaerobes 13. Furthermore, in both mammalian and non-mammalian species, enrofloxacin is partially metabolised in the liver to ciprofloxacin, a primary metabolite of which is cyclopropyl, a potent antimicrobial agent itself 14. The active substance is characterised by a low host toxicity, being non-mutagenic with a...

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“…Due to its pyogenic, opportunistic characteristic (taking advantage of a gateway), T. pyogenes causes suppurative infection in domestic and wild animals (RISSETI et al, 2017;ÇEÇEN et al, 2018;ROGOVSKYY et al, 2018), although virulence factors have not been fully elucidated (RISSETI et al, 2017;LIU et al, 2018;TAMAI et al, 2018). The evolution of the disease is related to cases of mastitis, arthritis, interdigital phlebitis, osteomyelitis, abortion, metritis, orchitis, omphalitis and bronchopneumonia as well as subcutaneous abscess or abscess in parenchymal tissues, with a variable prognosis (CAFFARO et al, 2014;ISHIYAMA et al, 2017;WARETH et al, 2018;KONTTURI et al, 2019;PIERSANTI et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Subcutaneous abscess caused by Trueperella pyogenes in a bovine in the Western Amazon: case report

Reckziegel

Gomes

Aquino

et al. 2019

AVB

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Article history Trueperella pyogenes is a pathogenic bacterium that causes suppurative infections in domestic and wild animals as well as humans. This paper describes a clinical case of subcutaneous abscess by T. pyogenes in a bovine in the Western Amazon, Brazil. During anamnesis, intramuscular injectable drug administration in the middle third of the left gluteal biceps muscle and the practice of reusing needles were reported. The clinical examination revealed edema, hyperthermia and high pain sensitivity to the touch in the region. A sample was collected through a puncture incision for the bacterial culture, during which T. pyogenes was isolated. The therapeutic approach was the use of a systemic antimicrobial and topical care, with a good prognosis. This report is the first description of infection by T. pyogenes in cattle in the Western Amazon and underscores the importance of appropriate sanitary management in herds, especially the use of disposable needles and hygiene at the site of parenteral drug application.

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Virulence markers associated with Trueperella pyogenes infections in livestock and companion animals

Cited by 28 publications

References 14 publications

Phenotypic and genotypic characteristics of Trueperella pyogenes isolated from ruminants

Phenotypic and genotypic characteristics of Trueperella pyogenes isolated from ruminants

Effects of enrofloxacin treatment on the bacterial microbiota of milk from goats with persistent mastitis

Subcutaneous abscess caused by Trueperella pyogenes in a bovine in the Western Amazon: case report

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