Virulence genes, Shiga toxin subtypes, major O-serogroups, and phylogenetic background of Shiga toxin-producing Escherichia coli strains isolated from cattle in Iran

Jajarmi, Maziar; Fooladi, Abbas Ali Imani; Badouei, Mahdi Askari; Ahmadi, Ali

doi:10.1016/j.micpath.2017.05.041

Cited by 38 publications

(45 citation statements)

References 40 publications

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“…STEC are mostly associated with outbreaks and two important diseases, including haemorrhagic colitis (HC) and haemolytic uremic syndrome (HUS) [23]. Cattle are perceived as their primary reservoir and ajor source of food contamination [24,25]. In this study low frequency of STEC and EIEC (one infections was found We can hypothesize that our strains (diarrheal stool samples) analysedwere not related olitis and haemolytic uremic syndrome.…”

Section: Discussionmentioning

confidence: 62%

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Prevalence of <i>Escherichia coli</i> virulence genes in patients with diarrhoea in Ouagadougou, Burkina Faso

Somda¹,

Bonkoungou²,

Zongo³

et al. 2017

Af J Clin Exp Micro

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Objective: Diarrhoeagenic E. coli (DEC) strains are important causes of diarrhoea in the developing world and, to a lesser extent, inthe developed world. In this study, we investigated the prevalence of the virulence genes specific for five major pathogroups of diarrheagenic Escherichia coli (DEC) in primary cultures from diarrhoeagenic patients in Burkina Faso.Methodology: From September 2016 to Mars 2017, a total of 211 faecal samples from diarrhoeagenic patients from urban hospitals of Ouagadou, Burkina Faso have been analysed. A 16-plex PCR was used to detect simultaneously, the five major DEC pathotypes (enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), Shiga toxin-producing E. coli (STEC), enteroaggregative E. coli (EAEC) and enteroinvasive E. coli (EIEC)). Results: At least one diarrhoeagenic E. Coli pathotype was detected in 31 samples (14.7%) in children and adults with diarrhoea. EAEC was the most common pathotype detected 9.5% (20/211), followed by EIEC2.4% (05/211) and STEC 0.5% (01/211). More than one DEC pathotype were detected in 2.4% (05/211) patients. EPEC and ETEC were not detected in single infection but in co-infection with others pathotypes. Conclusion: DEC, especially enteroaggregative, may be important responsible of diarrhoeas in Burkina Faso from all ages patient.Key Words: Diarrhoeagenic Escherichia coli, 16-plex PCR, Burkina Faso, human diarrhoeas stool. PREVALENCE DES GENES DE VIRULENCE D'ESCHERICHIA COLIISOLEES DES SELLES DIARRHEIQUES CHEZ LES PATIENTS

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Section: Discussionmentioning

confidence: 62%

“…PCR was performed in a reaction of 20 µl containing 2.5µl 10 X PCR buffer (SolisBiodyne), 0.75µl dNTPs (10mM), 0. 25 …”

Section: Cultivation Samplesmentioning

confidence: 99%

Prevalence of <i>Escherichia coli</i> virulence genes in patients with diarrhoea in Ouagadougou, Burkina Faso

Somda¹,

Bonkoungou²,

Zongo³

et al. 2017

Af J Clin Exp Micro

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“…Stx2 positive strain especially Stx2a induces more severe clinical symptoms and higher mortality than other Stx subtypes 25 . Previous study reported that 20% of meat STEC isolates carried eae 19 , 36% of the cattle isolates possessed eae 26 . In this study, we found that 6.7% of raw meat isolates possessed eae, the prevalence of eae in cattle isolates is 26.6%.…”

Section: Discussionmentioning

confidence: 93%

Genetic diversity of the intimin gene (eae) in non-O157 Shiga toxin-producing Escherichia coli strains in China

Yang

Sun

Fan

et al. 2020

Sci Rep

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Shiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen. The increasing incidence of non-O157 STEC has posed a great risk to public health. Besides the Shiga toxin (Stx), the adherence factor, intimin, coded by eae gene plays a critical role in STEC pathogenesis. In this study, we investigated the prevalence and polymorphisms of eae gene in non-O157 STEC strains isolated from different sources in China. Among 735 non-O157 STEC strains, eae was present in 70 (9.5%) strains. Eighteen different eae genotypes were identified in 62 eae-positive STEC strains with the nucleotide identities ranging from 86.01% to 99.97%. Among which, seven genotypes were newly identified in this study. The eighteen eae genotypes can be categorized into five eae subtypes, namely β1, γ1, ε1, ζ3 and θ. Associations between eae subtypes/genotypes and serotypes as well as origins of strains were observed in this study. Strains belonging to serotypes O26:H11, O103:H2, O111:H8 are associated with particular eae subtypes, i.e., β1, ε1, θ, respectively. Most strains from diarrheal patients (7/9, 77.8%) carried eae-β1 subtype, while most isolates from cattle (23/26, 88.5%) carried eae-ζ3 subtype. This study demonstrated a genetic diversity of eae gene in non-O157 STEC strains from different sources in China.

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“…For example, outbreaks of E. coli O157:H7 in the United States have occurred due consumption of a variety of fresh produce including onions, bagged spinach, lettuce and unpasteurized apple juice [10–12]. Produce and water supplies used for irrigation or consumption can become contaminated by coming in contact with trace amounts of fecal waste from farm animals, which can be carriers of E. coli O157:H7 but are themselves unaffected by it [6, 13].…”

Section: Introductionmentioning

confidence: 99%

Development and on-site evaluation of an easy-to-perform and low-cost food pathogen diagnostic workflow for low-resource communities

Mason

Botella

2020

Preprint

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20Food-borne diseases are a leading cause of illness and death in many developing 21 countries and thus, there is a real need to develop affordable and practical 22 technologies that can help improve food safety in these countries. The ability to 23 efficiently identify food pathogens is essential to allow national regulatory authorities 24 to monitor food quality and implement safety protocols. In this study, we have 25 developed a simple, low-cost ($0.76 (USD)) complete food pathogen diagnostic 26 workflow ideally suited for deployment in low-resource environments that uses a 27 simple four step process (sample enrichment, cell lysis, DNA amplification, and 28 naked-eye readout). The minimal number of steps and equipment involved in our 29 diagnostic workflow, as well as the simplicity of the yes/no flocculation readout, 30 allows non-technical personnel to perform and interpret the assay. To evaluate the 31 system's performance, we tested the entire system on fresh produce samples 32 collected from local farms and markets in Cambodia for the presence of the E. coli 33 O157 O-antigen polymerase, wzy. Although this was a proof-of-concept study, our 34 system successfully revealed a clear correlation between the origin and condition of 35 the produce collected and their likelihood of contamination. In conclusion, we believe 36 that our easy-to-perform diagnostic system can have a significant impact on 37 improving food quality and human health if adopted by regulatory authorities in 38 developing countries due to the assay's simplicity, affordability, and adaptability. 3 40 Introduction 41Over the last 20 years, there has been a significant increase in the incidence of food-42 borne diseases worldwide [1]. The risk of infection by a food-borne disease is 43 significantly higher in developing countries due to a combination of factors including 44 access to clean water and bathroom facilities, poor hygiene education, inadequate 45 food production and storage practices, and either insufficient food safety legislation 46 or poor implementation of existing legislation [2]. Most food-borne disease infections 47 in these countries result from the consumption of perishable foods sold in informal 48 markets [3] and, as such, food-borne diseases have become a leading cause of 49 illness and death in developing countries [4, 5]. Hence, there is a need for low-cost 50 and simple technologies that can help health authorities to monitor and enforce 51 adequate levels of food safety. In addition to the health benefits, increased food 52 safety standards would likely have economic benefits as a result of increased 53 demand for fresh produce exports [3]. 54 55 Shiga toxin-producing Escherichia coli (STEC) are a class of E. coli responsible for 56 many food-borne outbreaks and sporadic cases of gastrointestinal illness, with a 57 range of symptoms including haemorrhagic colitis (stomach cramping and bloody 58 diarrhoea) and the potentially fatal hemolytic-uremic syndrome (break down of red 59 blood cells, kidney failure, re...

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Virulence genes, Shiga toxin subtypes, major O-serogroups, and phylogenetic background of Shiga toxin-producing Escherichia coli strains isolated from cattle in Iran

Cited by 38 publications

References 40 publications

Prevalence of <i>Escherichia coli</i> virulence genes in patients with diarrhoea in Ouagadougou, Burkina Faso

Prevalence of <i>Escherichia coli</i> virulence genes in patients with diarrhoea in Ouagadougou, Burkina Faso

Genetic diversity of the intimin gene (eae) in non-O157 Shiga toxin-producing Escherichia coli strains in China

Development and on-site evaluation of an easy-to-perform and low-cost food pathogen diagnostic workflow for low-resource communities

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