Virulence genes identification and characterization revealed the presence of the Yersinia High Pathogenicity Island (HPI) in Salmonella from Brazil

Santos, Anamaria M.P. dos; Ferrari, Rafaela Gomes; Panzenhagen, Pedro; Rodrigues, Grazielle Lima; Conté-Júnior, Carlos Adam

doi:10.1016/j.gene.2021.145646

Cited by 20 publications

(12 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most virulence‐related genes in Salmonella are found in Salmonella Pathogenicity Islands (SPIs). SPIs cover large regions of the chromosome that encode several virulence factors (Dos Santos et al., 2021) and, so far, 17 SPIs have been described (Fowoyo, 2020).…”

Section: Principal Textmentioning

confidence: 99%

Salmonella spp. in low water activity food: Occurrence, survival mechanisms, and thermoresistance

Morasi

Rall

Dantas

et al. 2022

Journal of Food Science

View full text Add to dashboard Cite

The occurrence of disease outbreaks involving low‐water‐activity (aw) foods has gained increased prominence due in part to the fact that reducing free water in these foods is normally a measure that controls the growth and multiplication of pathogenic microorganisms. Salmonella, one of the main bacteria involved in these outbreaks, represents a major public health problem worldwide and in Brazil, which highlights the importance of good manufacturing and handling practices for food quality. The virulence of this pathogen, associated with its high ability to persist in the environment, makes Salmonella one of the main challenges for the food industry. The objectives of this article are to present the general characteristics, virulence, thermoresistance, control, and relevance of Salmonella in foodborne diseases, and describe the so‐called low‐water‐activity foods and the salmonellosis outbreaks involving them.

show abstract

Section: Principal Textmentioning

confidence: 99%

Salmonella spp. in low water activity food: Occurrence, survival mechanisms, and thermoresistance

Morasi

Rall

Dantas

et al. 2022

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…Studies involving whole-genome sequencing (WGS) have facilitated the biological understanding of gastrointestinal pathogens and several existing microorganisms. The advances of WGS generated by high throughput sequencing platforms has allowed the mass submission of genomes to public repositories in the last years [17,18]. Consequently, in silico studies from bacterial genomes have increased in the last few years, providing several possibilities in gene analysis [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

The Distribution of Campylobacter jejuni Virulence Genes in Genomes Worldwide Derived from the NCBI Pathogen Detection Database

Panzenhagen

Portes

Santos

et al. 2021

Genes

Self Cite

View full text Add to dashboard Cite

Campylobacter jejuni (C. jejuni) is responsible for 80% of human campylobacteriosis and is the leading cause of gastroenteritis globally. The relevant public health risks of C. jejuni are caused by particular virulence genes encompassing its virulome. We analyzed 40,371 publicly available genomes of C. jejuni deposited in the NCBI Pathogen Detection Database, combining their epidemiologic metadata with an in silico bioinformatics analysis to increase our current comprehension of their virulome from a global perspective. The collection presented a virulome composed of 126 identified virulence factors that were grouped in three clusters representing the accessory, the softcore, and the essential core genes according to their prevalence within the genomes. The multilocus sequence type distribution in the genomes was also investigated. An unexpected low prevalence of the full-length flagellin flaA and flaB locus of C. jejuni genomes was revealed, and an essential core virulence gene repertoire prevalent in more than 99.99% of genomes was identified. Altogether, this is a pioneer study regarding Campylobacter jejuni that has compiled a significant amount of data about the Multilocus Sequence Type and virulence factors concerning their global prevalence and distribution over this database.

show abstract

“…The role of shdA in S . typhimurium is mainly participated in adherence/invasion of fibronectin-producing cells ( Dos et al, 2021 ), and shdA -mediated binding of the extracellular matrix contributes to persistent intestinal carriage ( Kingsley et al, 2000 ; Urrutia et al, 2014 ). Interestingly, the study found that shd A gene were only carried in outbreak strains, but not in other strains located in the same evolutionary branch as the outbreak strain.…”

Section: Discussionmentioning

confidence: 99%

A Severe Gastroenteritis Outbreak of Salmonella enterica Serovar Enteritidis Linked to Contaminated Egg Fried Rice, China, 2021

et al. 2021

View full text Add to dashboard Cite

Salmonella contamination of eggs and egg shells has been identified as a public health problem worldwide. Here, we reported an outbreak of severe gastrointestinal symptoms caused by Salmonella enterica serovar Enteritidis (S. enteritidis) in China. We evaluated the outbreak by using epidemiological surveys, routine laboratory testing methods, and whole genome sequencing (WGS). This outbreak occurred in a canteen in Beijing, during March 9–11, 2021, 225 of the 324 diners who have eaten at the canteen showed gastrointestinal symptoms. The outbreak had characteristical epidemiological and clinical features. It caused a very high attack rate (69.4%) in a short incubation time. All patients developed diarrhea and high fever, accompanied by abdominal pain (62.3%), nausea (50.4%), and vomiting (62.7%). The average frequency of diarrhea was 12.4 times/day, and the highest frequency of diarrhea was as high as 50 times/day. The average fever temperature was 39.4°C, and the highest fever temperature was 42°C. Twenty strains of S. enteritidis were recovered, including 19 from the patients samples, and one from remained egg fried rice. Antibiotic susceptibility test showed that the 20 outbreak strains all had the same resistance pattern. PFGE results demonstrated that all 20 strains bore completely identical bands. Phylogenetic analysis based on WGS revealed that all 20 outbreak strains were tightly clustered together. So the pathogenic source of this food poisoning incident may was contaminated egg fried rice. Resistance gene analysis showed that the outbreak strains are all multi-drug resistant strains. Virulence gene analysis indicated that these outbreak strains carried a large number of virulence genes, including 2 types of Salmonella pathogenicity islands (SPI-1 and SPI-2). Other important virulence genes were also carried by the outbreak strains, such as pefABCD, rck and shdA. And the shdA gene was not in other strains located in the same evolutionary branch as the outbreak strain. We speculated that this is a significant reason for the serious symptoms of gastroenteritis in this outbreak. This outbreak caused by S. enteritidis suggested government should strengthen monitoring of the prevalence of outbreak clone strains, and take measures to mitigate the public health threat posed by contaminated eggs.

show abstract

Virulence genes identification and characterization revealed the presence of the Yersinia High Pathogenicity Island (HPI) in Salmonella from Brazil

Cited by 20 publications

References 85 publications

Salmonella spp. in low water activity food: Occurrence, survival mechanisms, and thermoresistance

Salmonella spp. in low water activity food: Occurrence, survival mechanisms, and thermoresistance

The Distribution of Campylobacter jejuni Virulence Genes in Genomes Worldwide Derived from the NCBI Pathogen Detection Database

A Severe Gastroenteritis Outbreak of Salmonella enterica Serovar Enteritidis Linked to Contaminated Egg Fried Rice, China, 2021

Contact Info

Product

Resources

About