Virulence genes and enterobacterial repetitive intergenic consensus region (ERIC) profiling reveals highly diverse genetic population among avian strains of Pasteurella multocida

Karthik, Kumaragurubaran; Devi, Rajan Prasanna; Chitra, M. Ananda; Kalaiselvi, G.; Bharathi, Ramasamy; Sridhar, R.

doi:10.1016/j.micpath.2021.105303

Cited by 2 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, to roughly study the genetic relationship of S. pneumoniae with a limited budget and time constraints along with an uncomplicated procedure, this method may provide an option as a molecular typing tool. Currently, there are several studies that have applied this method for genotypic characterization with promising results, such as for Acinetobacter baumannii [12,15], Corynebacterium pseudotuberculosis [16], Escherichia coli [17], Haemophilus parasuis [18], Pasteurella multocida [19], Pseudomonas aeruginosa [14], Shigella [20], Vibrio parahaemolyticus [21], and viridans streptococci [22]. There was only one report which used the ERIC method for different clonalities of S. pneumoniae [29].…”

Section: Discussionmentioning

confidence: 99%

“…ERIC-PCR is a simple, sharp, and costeffective genotyping technology that discriminates different types of bacterial isolates. The widespread distribution of these repetitive DNA elements of various bacteria should enable the rapid identification of bacterial species and isolates [12][13][14][15], which is useful for the analysis of bacterial genomes [16][17][18][19]. The ERIC sequences can be utilized as efficient primer binding sites in a PCR to produce fingerprints of different bacterial genomes [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Study of Streptococcus pneumoniae serotype 19A blood isolates from Thailand by enterobacterial repetitive intergenic consensus (ERIC)-PCR typing

Yungyuen,

Chongtrakool,

Phongsamart

et al. 2023

ScienceAsia

View full text Add to dashboard Cite

Streptococcus pneumoniae serotype 19A is frequently isolated worldwide. In this study, the clonal relationships among 62 isolates from different patients from 21 hospitals between 2008 and 2018 were characterized using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The different band patterns that appeared upon agarose gel electrophoresis were used to construct an unweighted pair group method with an arithmetic mean (UPGMA) dendrogram. There were 23 different ERIC types (E1-E23). The most prevalent type was E9, accounting for 19.36% of all isolates, followed by E6 at 16.13%, E5 at 11.30%, and 13 ERIC types present in only one isolate or at 1.61% each. Using an additional study to determine the clonal relationships, we compared our ERIC-PCR results to the corresponding multilocus sequence types (MLSTs) from our recent study with the same 62 S. pneumoniae serotype 19A isolates. The results showed there were 20 different MLST types and that ERIC-PCR was comparable to MLST as a valuable complementary tool for the investigation of S. pneumoniae serotype 19A isolates. Furthermore, ERIC-PCR is very fast, affordable, and easy to perform compared to MLST. However, there is less concordance between these two methods. These results suggest a high diversity of different ERIC-PCR and MLST patterns. Overall, the combination of results from both methods can add greater discrimination and complementary information for the differentiation of S. pneumoniae strains in Thailand.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Study of Streptococcus pneumoniae serotype 19A blood isolates from Thailand by enterobacterial repetitive intergenic consensus (ERIC)-PCR typing

Yungyuen,

Chongtrakool,

Phongsamart

et al. 2023

ScienceAsia

View full text Add to dashboard Cite

show abstract

Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks

Bernal

Fernández

Arnal

et al. 2022

Animals

View full text Add to dashboard Cite

Studies that characterize bovine respiratory disease (BRD)-associated Pasteurella multocida isolates are scarce compared with research on isolates from other hosts and clinical backgrounds. In the present study, 170 P. multocida isolates from 125 BRD outbreaks were characterized by capsular and LPS typing as well as by virulotyping. Three capsular types (A, B, F) and three LPS genotypes (L2, L3, L6) were identified. Capsular and LPS typing revealed a very low genetic diversity (GD = 0.02) among P. multocida, with most isolates belonging to genotype A:L3 (97.6%). Virulotyping identified seven virulence-associated gene profiles, with two profiles including 95.9% of the isolates. A subset of isolates was further characterized by MLST and PFGE. The sequence types ST79 and ST13 were the most frequently identified and were grouped into the same clonal complex (CC13), a result that supports the clonal population structure of BRD-associated P. multocida isolates. PFGE typing also revealed a low genetic diversity (GD = 0.18), detecting a single pattern in 62.5% of the outbreaks in which multiple isolates were analyzed. Overall, 85.2% of the isolates belonged to pulsotypes with at least 80% genetic similarity, consistent with a clonal population structure observed by MLST analysis and corroborating the genetic relatedness of most P. multocida isolates associated with BRD in cattle.

show abstract

Virulence genes and enterobacterial repetitive intergenic consensus region (ERIC) profiling reveals highly diverse genetic population among avian strains of Pasteurella multocida

Cited by 2 publications

References 25 publications

Study of Streptococcus pneumoniae serotype 19A blood isolates from Thailand by enterobacterial repetitive intergenic consensus (ERIC)-PCR typing

Study of Streptococcus pneumoniae serotype 19A blood isolates from Thailand by enterobacterial repetitive intergenic consensus (ERIC)-PCR typing

Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks

Contact Info

Product

Resources

About