Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine

Abstract

Pasteurella multocida causes atrophic rhinitis in swine and fowl cholera in birds, and is a secondary agent in respiratory syndromes. Pathogenesis and virulence factors involved are still poorly understood. The aim of this study was to detect 22 virulence-associated genes by PCR, including capsular serogroups A, B and D genes and to evaluate the antimicrobial susceptibility of P. multocida strains from poultry and swine. ompH, oma87, plpB, psl, exbD-tonB, fur, hgbA, nanB, sodA, sodC, ptfA were detected in more than 90% of the strains of both hosts. 91% and 92% of avian and swine strains, respectively, were classified in serogroup A. toxA and hsf-1 showed a significant association to serogroup D; pmHAS and pfhA to serogroup A. Gentamicin and amoxicillin were the most effective drugs with susceptibility higher than 97%; however, 76.79% of poultry strains and 85% of swine strains were resistant to sulphonamides. Furthermore, 19.64% and 36.58% of avian and swine strains, respectively, were multi-resistant. Virulence genes studied were not specific to a host and may be the result of horizontal transmission throughout evolution. High multidrug resistance demonstrates the need for responsible use of antimicrobials in animals intended for human consumption, in addition to antimicrobial susceptibility testing to P. multocida.

Çin’de Kanatlı Hayvanlardan İzole Edilen Pasteurella multocida Suşlarının Moleküler Karakterizasyonu ve tonB Geni Açısından Genetik Analizi

“…The results showed that the capsular types of isolates were predominantly type A which is similar to that it was usual reported for P. multocida in China and other countries [34,35] . But we found one isolate of P. multocida was type F which is similar to the findings that reported previously [33] . Meanwhile, in these two provinces, antibiotic therapy is still an effective tool in the treatment of infections caused by P. multocida.…”

“…Parallel with the developments on the research of P. multocida, it has been almost completely controlled. Nevertheless, the pathogenic mechanism is currently not well understood, and thus it still restricts the development of livestock and poultry breeding and causes severe economic losses [9,33] . In this study, 23 strains of P. multocida isolated from poultry in Chongqing and Sichuan, China were tested for capsular antigens and antimicrobial susceptibility.…”

“…This result may indicate that the avian strain or serotype A of P. multocida is not easy to carry the toxA gene. Some research suggested that the toxA gene is more significantly associated with serotype D, compared to serotype A [33,36] . We investigated iron-regulated and Characteristics Analysis of P. multocida Strains iron-acquisition related genes (tonB, fur, hgbB, hgbA) irrespective of the animal clinical status and found that all isolates had the tonB gene similar to the findings reported previously [37] .…”

“…The phylogenetic tree displayed that these tonB genes have close genetic relationship and there is a high similarity (92.9%-100%) among these tonB genes. A similar distribution of certain genes, regardless of the host species of P. multocida serotype, may suggest that the selection of factors that present cross-protection as candidates for vaccine development [33,42] . TonB protein is generally conserved among species and has a high immunogenicity.…”

Evaluation of the biofilm formation capacity of Pasteurella multocida strains isolated from cases of fowl cholera and swine lungs and its relationship with pathogenicity

“…To amplify the tadD gene, we used the PCR protocol described by Furian et al (2016). The amplification of the tadB, tadE and tadG genes was adapted from the protocol described by Moraes et al (2014).…”