“…A similar scenario unfolded in Finland when trimethoprim was withdrawn from use for the treatment of UTI ( Heikkila et al, 1990 ). In the Enterobacteriaceae, resistance to sulfonamides, trimethoprim, ampicillin, tetracycline, and fluoroquinolones remains high ( Enne et al, 2001 ; Nozarian and Abdollahi, 2015 ; Paniagua-Contreras et al, 2015 ; Uzun et al, 2015 ; Yahiaoui et al, 2015 ). Carriage rates for sul1 , sul2 , or sul3 genes in E. coli isolated from retail meat, livestock, healthy humans, and humans with clinical disease also remains a problem ( Enne et al, 2001 ; Blahna et al, 2006 ; Frank et al, 2007 ; Altalhi et al, 2010 ; Bailey et al, 2010 ; Saenz et al, 2010 ; Vogt et al, 2014 ; Yahiaoui et al, 2015 ).…”
Section: Antimicrobial Resistance In
Escherichia Colimentioning
Contamination of waste effluent from hospitals and intensive food animal production with antimicrobial residues is an immense global problem. Antimicrobial residues exert selection pressures that influence the acquisition of antimicrobial resistance and virulence genes in diverse microbial populations. Despite these concerns there is only a limited understanding of how antimicrobial residues contribute to the global problem of antimicrobial resistance. Furthermore, rapid detection of emerging bacterial pathogens and strains with resistance to more than one antibiotic class remains a challenge. A comprehensive, sequence-based genomic epidemiological surveillance model that captures essential microbial metadata is needed, both to improve surveillance for antimicrobial resistance and to monitor pathogen evolution. Escherichia coli is an important pathogen causing both intestinal [intestinal pathogenic E. coli (IPEC)] and extraintestinal [extraintestinal pathogenic E. coli (ExPEC)] disease in humans and food animals. ExPEC are the most frequently isolated Gram negative pathogen affecting human health, linked to food production practices and are often resistant to multiple antibiotics. Cattle are a known reservoir of IPEC but they are not recognized as a source of ExPEC that impact human or animal health. In contrast, poultry are a recognized source of multiple antibiotic resistant ExPEC, while swine have received comparatively less attention in this regard. Here, we review what is known about ExPEC in swine and how pig production contributes to the problem of antibiotic resistance.
“…A similar scenario unfolded in Finland when trimethoprim was withdrawn from use for the treatment of UTI ( Heikkila et al, 1990 ). In the Enterobacteriaceae, resistance to sulfonamides, trimethoprim, ampicillin, tetracycline, and fluoroquinolones remains high ( Enne et al, 2001 ; Nozarian and Abdollahi, 2015 ; Paniagua-Contreras et al, 2015 ; Uzun et al, 2015 ; Yahiaoui et al, 2015 ). Carriage rates for sul1 , sul2 , or sul3 genes in E. coli isolated from retail meat, livestock, healthy humans, and humans with clinical disease also remains a problem ( Enne et al, 2001 ; Blahna et al, 2006 ; Frank et al, 2007 ; Altalhi et al, 2010 ; Bailey et al, 2010 ; Saenz et al, 2010 ; Vogt et al, 2014 ; Yahiaoui et al, 2015 ).…”
Section: Antimicrobial Resistance In
Escherichia Colimentioning
Contamination of waste effluent from hospitals and intensive food animal production with antimicrobial residues is an immense global problem. Antimicrobial residues exert selection pressures that influence the acquisition of antimicrobial resistance and virulence genes in diverse microbial populations. Despite these concerns there is only a limited understanding of how antimicrobial residues contribute to the global problem of antimicrobial resistance. Furthermore, rapid detection of emerging bacterial pathogens and strains with resistance to more than one antibiotic class remains a challenge. A comprehensive, sequence-based genomic epidemiological surveillance model that captures essential microbial metadata is needed, both to improve surveillance for antimicrobial resistance and to monitor pathogen evolution. Escherichia coli is an important pathogen causing both intestinal [intestinal pathogenic E. coli (IPEC)] and extraintestinal [extraintestinal pathogenic E. coli (ExPEC)] disease in humans and food animals. ExPEC are the most frequently isolated Gram negative pathogen affecting human health, linked to food production practices and are often resistant to multiple antibiotics. Cattle are a known reservoir of IPEC but they are not recognized as a source of ExPEC that impact human or animal health. In contrast, poultry are a recognized source of multiple antibiotic resistant ExPEC, while swine have received comparatively less attention in this regard. Here, we review what is known about ExPEC in swine and how pig production contributes to the problem of antibiotic resistance.
“…coli recovered from cystitis and 36 to 48 % from pyelonephritis . This toxin is also implicated in acute prostatitis and is associated with ampicillin‐resistant strains . CNF1 production by K1 strains enhances the invasion of bovine mammary epithelial cells and of the blood–brain barrier …”
Cytotoxic necrotizing factor 1 (CNF1) is a toxin produced by pathogenic strains of Escherichia coli responsible for extra-intestinal infections. CNF1 deamidates Rac1, thereby triggering its permanent activation and worsening inflammatory reactions. Activated Rac1 is prone to proteasomal degradation. There is no targeted therapy against CNF1, despite its clinical relevance. In this work we developed a fluorescent cell-based immunoassay to screen for inhibitors of CNF1-induced Rac1 degradation among 1120 mostly approved drugs. Eleven compounds were found to prevent CNF1-induced Rac1 degradation, and five also showed a protective effect against CNF1-induced multinucleation. Finally, lasalocid, monensin, bepridil, and amodiaquine protected cells from both diphtheria toxin and CNF1 challenges. These data highlight the potential for drug repurposing to fight several bacterial infections and Rac1-based diseases.
“…In the ExPEC uropathogenic E. coli (UPEC) strains, expression of type 1 pili enhances colonization of the urothelial mucosa, promotes biofilm formation and host cell invasion, and induces expression of proinflammatory cytokines ( 18 – 21 ). Moreover, some UPEC strains are able to persist within infected tissues due to their ability to inhibit NF-κB activation, modulate expression and release of proinflammatory cytokines ( 22 – 24 ), and form intracellular bacterial communities, which allows bacteria to resist the host immune response and antibiotic therapy ( 25 – 28 ). On the other hand, it has been shown that AIEC strains are stronger biofilm producers ( 29 ) and can subvert the innate immune response ( 30 ), allowing them to persistently colonize the intestinal mucosa.…”
Adherent/invasive Escherichia coli (AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than in healthy subjects. Since AIEC strains show a high percentage of similarity to extraintestinal pathogenic E. coli (ExPEC), neonatal meningitis-associated E. coli (NMEC), and uropathogenic E. coli (UPEC) strains, here we compared AIEC strain LF82 with a UPEC isolate (strain EC73) to assess whether LF82 would be able to infect prostate cells as an extraintestinal target. The virulence phenotypes of both strains were determined by using the RWPE-1 prostate cell line. The results obtained indicated that LF82 and EC73 are able to adhere to, invade, and survive within prostate epithelial cells. Invasion was confirmed by immunofluorescence and electron microscopy. Moreover, cytochalasin D and colchicine strongly inhibited bacterial uptake of both strains, indicating the involvement of actin microfilaments and microtubules in host cell invasion. Moreover, both strains belong to phylogenetic group B2 and are strong biofilm producers. In silico analysis reveals that LF82 shares with UPEC strains several virulence factors: namely, type 1 pili, the group II capsule, the vacuolating autotransporter toxin, four iron uptake systems, and the pathogenic island (PAI). Furthermore, compared to EC73, LF82 induces in RWPE-1 cells a marked increase of phosphorylation of mitogen-activated protein kinases (MAPKs) and of NF-κB already by 5 min postinfection, thus inducing a strong inflammatory response. Our in vitro data support the hypothesis that AIEC strains might play a role in prostatitis, and, by exploiting host-cell signaling pathways controlling the innate immune response, likely facilitate bacterial multiplication and dissemination within the male genitourinary tract.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.