Virulence factors of Escherichia coli isolated from bovine clinical mastitis

Kaipainen, Tarja; Pohjanvirta, Tarja; Shpigel, Nahum Y.; Shwimmer, A.; Pyörälä, Satu; Pelkonen, Sinikka

doi:10.1016/s0378-1135(01)00483-7

Cited by 67 publications

(74 citation statements)

References 18 publications

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“…Although, the rates of traT gene vary depending on the country, it is the most commonly obtained virulence factor in E. coli strains. Likewise, traT gene prevalence in previous studies varied from 36% to 62% [10,16]. In the current study, a higher rate (75%) was detected in the observed strains.…”

Section: Discussionsupporting

confidence: 45%

“…The presence of the aerobactin gene was evaluated in previous studies [10,17,18]. Although, Lin et al (1998) defi ned the gene in all strains of E. coli, some researchers [10,18] suggested smaller rates (4-11%) of aerobactin gene presence. Conversely, none of the strains carried the gene in the current study.…”

Section: Discussionmentioning

confidence: 99%

“…The amplifi cation conditions included 25 cycles of denaturation at 95°C for 2 minutes, primer annealing at 55°C for 1 minute, and extension at 72°C for 1 minute. The fi nal extension step was 10 minutes at 70°C [10].…”

Section: Pcr Detection Of Virulence Genesmentioning

confidence: 99%

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Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

2014

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Cow mastitis caused by Escherichia coli (E. coli) exhibits various local and systemic clinical signs at varying degrees of severity. The aim this study was to elucidate the virulence properties, antibiotic resistance and phylogenetics of 56 E. coli strains. Of all the studied strains, 12 were positive for hemolytic properties and 38 were positive for biofi lm production. Additionally, 55 of the strains were positive for multiple resistances in bacteriological tests. PCR analysis revealed that 42 strains carried the traT gene, 20 strains had the shiga toxin gene (stx1-stx2), and 8 strains carried the intimin gene (eae), but all strains were negative for aerobactin gene (aer). All strains encoding shiga toxin genes were also positive for stx1, but only 4 strains were positive for stx2. There were no signifi cant differences in virulence genes between antibiotic-resistant and antibiotic-susceptible strains. The random amplifi ed polymorphic DNA-polymerase chain reaction patterns revealed the existence of 13 main groups with 4 subgroups of E. coli. In this study, E. coli strains causing intramammary infections and originating from various sources might show resistance against common antibiotics. Pathogenity of E. coli that cause clinical mastitis, and prognosis of the infection might be predicted by obtaining the traT gene. Additionally, antibiotic resistance should be investigated at the genomic level to detect the relationship between virulence factors and antibiotic resistance. In fi eld conditions, development of antibiotic resistance is the main cause of mastitis treatment failure. Thus, antibiotic resistance profi les in herds should be monitored and effective antibiotics should be administered.

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Section: Discussionsupporting

confidence: 45%

Section: Discussionmentioning

confidence: 99%

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Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

2014

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“…This discrepancy may result from differences in the assessment of the degree of serum resistance, such as the percentage of serum used in the assay, or the technique used to assess the survival of bacteria. This is not the only explanation, because in one study, the percentage of serum-resistant isolates varied from high to low according to the geographical origin of the strains [55]. In the absence of a standardized assay, the degrees of resistance or susceptibility of bacterial isolates are impossible to compare from one study to another.…”

Section: The Biological Significance Of the Complement In Milk And Armentioning

confidence: 99%

The complement in milk and defense of the bovine mammary gland against infections

Rainard

2003

Vet. Res.

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-The mammary gland of dairy cows, which is prone to infection by various bacteria, mobilizes local and systemic immune defenses to cope with pathogens. The complement system plays an important part in the innate immunity against microorganisms through its bactericidal, opsonic, and phlogistic functions. The amount of the complement in the milk of healthy glands of dairy cows is low. Moreover, the classical pathway of activation is not functional because of a shortage in C1q. By contrast, the alternative pathway is active, deposits C3b and C3bi on bacteria, and generates amounts of C5a which are highly variable among cows. A slight inhibition of the bactericidal/hemolytic activities, of the deposition of C1q on bacteria, and of the phlogistic activity of C5a makes milk a rather anti-inflammatory fluid. The inhibitory activity does not involve C3b/ C3bi deposition on bacteria, nor the generation of C5a by the alternative pathway. When inflammation develops, the blood-derived complement components overcome the inhibitions and complement-dependent bactericidal, opsonic and phlogistic activities may be high in milk. Further research is necessary to evaluate the contribution of C5a to the recruitment of leukocytes in the mammary gland, and to specify the links between the complement system and the response of resident cells (leukocytes and mammary epithelial cells) to infection stimulus. This will help to define the contribution of the complement system to resistance against mastitis, and could help to differentiate animals more or less resistant to this frequent and costly disease.

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“…Serotyping has long been accepted as a common method for estimating the pathogenic potential of APEC strains, by which a number of serotypes, such as O1, O2, O8, O18 and O78, were detected more frequently than other serotypes (Ewers et al, 2004(Ewers et al, , 2007McPeake et al, 2005;Rodriguez-Siek et al, 2005b;Vandekerchove et al, 2005). A close relationship between virulence factors and pathogencity of APEC has been reported (Babai et al, 1997;Gomis et al, 2001;Janben et al, 2001;Kaipainen et al, 2002;Dziva & Stevens, 2008;Tyler et al, 2008). Genotyping of the virulence-associated genes has been suggested to differentiate APEC strains isolated from internal organs of poultry suffering multi-systemic infections from E. coli strains from faeces of clinically healthy chickens (Pfaff-McDonough et al, 2000;Delicato et al, 2003;McPeake et al, 2005;Rodriguez-Siek et al, 2005b;Skyberg et al, 2007;Johnson et al, 2008).…”

Section: Introductionmentioning

confidence: 97%

Distribution of serotypes and virulence-associated genes in pathogenicEscherichia coliisolated from ducks

et al. 2010

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The objective of the present study was to investigate the serotypes and virulence-associated genes of avian pathogenic Escherichia coli (APEC) isolated from duck colibacillosis cases. Two hundred and fifty-four APEC isolates from duck colibacillosis cases were serotyped and amplified for 12 known virulenceassociated genes and the betA gene (encoding choline dehydrogenase) by polymerase chain reaction assays. One hundred and forty-three E. coli isolates from cloacal swabs of healthy ducks were also amplified for the same genes. A total of 53 O-serogroups were found in 254 APEC isolates, among which O93, O78 and O92 were predominant serogroups. Polymerase chain reaction results showed that Shiga-toxin-producing E. coli distributed in only 2.4% of ducks compared with 49.2% of the APEC isolates harbouring the irp2 gene, and 44.9% the fyuA gene, respectively. The ibeA gene was only present in 27 APEC isolates and was not found in healthy ducks. The rfaH gene was detected in 20.5% of APEC isolates, whereas 5.6% was found in healthy ducks. A total 79.5% of APEC isolates harboured the betA gene, which was significantly higher than in healthy ducks (16.1%), suggesting that betA may be associated with virulence.

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Virulence factors of Escherichia coli isolated from bovine clinical mastitis

Cited by 67 publications

References 18 publications

Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

The complement in milk and defense of the bovine mammary gland against infections

Distribution of serotypes and virulence-associated genes in pathogenicEscherichia coliisolated from ducks

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