Virulence Factors of Avian Pathogenic Escherichia coli Isolated from Broilers from the South of Brazil

Rocha, Ângela Sanches; Silva, Ari Bernardes da; Brito, A Benito Guimarães de; Moraes, Hamilton Luiz de Souza; Pontes, Alexandre Pontes; Cé, Milene C.; Nascimento, Vladimir Pinheiro do; Salle, Carlos Tadeu Pippi

doi:10.1637/0005-2086(2002)046[0749:vfoape]2.0.co;2

Cited by 16 publications

(13 citation statements)

References 23 publications

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“…shown that these VGs are predominantly found on plasmids (Collingwood et al, 2014;da Rocha et al, 2002;Johnson et al, 2005;Nakazato et al, 2009). The presence of ExPEC-associated genes among the FEC and CEC isolates in the current study supports the hypothesis that clinical and faecal avian E. coli have zoonotic potential.…”

Section: Virulence Associated Genes In Faecal and Clinical Escherichisupporting

confidence: 87%

“…Previous studies have identified an association between the prevalence of different combinations of VGs and the pathogenicity of APEC (da Rocha et al, 2002;Ewers et al, 2004;Johnson et al, 2008b;Rocha et al, 2008). Further, they have reported that certain VG combinations are a useful tool to differentiate between APEC and AFEC (Johnson et al, 2008b;Rodriguez-Siek et al, 2005a;Schouler et al, 2012) when the E. coli was isolated from the lesions or the cloacal swabs of chickens affected with colibacillosis.…”

Section: Page | 106mentioning

confidence: 99%

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Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Section: Virulence Associated Genes In Faecal and Clinical Escherichisupporting

confidence: 87%

Section: Page | 106mentioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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“…Several authors dedicated themselves to these studies, and in spite of frequency variations, the following main factors are significant: adhesion capacity, colicin production, aerobactin presence, serum resistance, sensitive temperature hemagglutinin, and the presence of certain capsular antigens (Dho & Lafont 1982, Naveh et al 1984, Rocha et al 2002, Ngeleka et al 2002, Delicato et al 2003, McPeake et al 2005.…”

Section: Resultsmentioning

confidence: 99%

Genes associated with pathogenicity of avian Escherichia coli (APEC) isolated from respiratory cases of poultry

Rocha

Lima-Rosa

et al. 2008

Pesq. Vet. Bras.

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RESUMO.-[Genes associados à patogenicidade deEscherichia coli patogênica para aves (APEC) isoladas de frangos de corte com sintomatologia clínica respiratória.] Os mecanismos de virulência das amostras de Escherichia coli potencialmente patogênicas para aves (APEC) têm sido continuamente estudados e acredita-se ser multifatorial. Certas propriedades são associadas primariamente a amostras virulentas e vêm sendo identificadas em amostras de E. coli isoladas de aves. Neste estudo um total de 61 amostras de E. coli, isoladas de frangos de corte com problemas respiratórios, foram testadas através da Reação em Cadeia da Polimerase (PCR), para a presença dos genes responsáveis pela capacidade de adesão, fimbria P (papC) e fimbria F11 (felA), produção de colicinas (cvaC), presença de aerobactina (iutA), resistência sérica (iss), hemaglutinina temperatura sensível (tsh) e presença de dos antígenos capsulares K1 e K5 (kpsII). O gene iss foi detectado em 73,8%, tsh em 55,7%, iutA em 45,9%, felA em 39,3%, papC em 24,3%, cvaC em 23% e kpsII em 18%.

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“…The pathogenicity of an E.coli strain is based on the presence and expression of some potential virulence factors (Won et al, 2009). In spite of frequency variations, the following factors are significant including adhesion, invasion, colicin production, aerobactin presence, serum resistance and temperature sensitive haemagglutination (Dho and Lafont, 1982;Naveh et al, 1984;Rocha et al, 2002;Ngeleka et al, 2002;Brito et al, 2003;Delicato et al, 2003 andMcpeake et al, 2005). In this study, 15 E.coli isolates were obtained from chickens with confirmed cases of colibacillosis and were screened for 11 virulence genes commonly associated with APEC and we discussed the association between these virulence genes and phylogenetic groups in which E.coli belonged to(A, B1, B2 and D).…”

Section: Discussionmentioning

confidence: 99%

Molecular Screening Of Virulence Genes In Avian Pathogenic Esherichia Coli

Tawab

Ammar

Galil

2016

Benha Veterinary Medical Journal

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Avian pathogenic Escherichia coli (APEC) causes colibacillosis, which is one of the main causes of economic losses in the poultry industry worldwide. This disease occurs only when the E. coli infecting strain presents virulence factors (encoded by specific genes) that enable the adhesion and proliferation in the host organism. Herin, 15 E. coli strains of different serogroups isolated from birds with colibacillosis were assigned to their phylogenetic groups and analyzed for the occurrence of 11 virulence associated genes. phylogenetic typing showed that group B2 was the largest (33.3%, 5/15) followed by group B1 (26.7%, 4/15), groups A and D were similar in size (22.1%, 19/86). The virulence profiles showed that ompA was found in most isolates 14/15 (93.3%). The iss gene was found in13/15 (86.6%). Followed by traT and iutA genes which were found in12/15 (80%). cvaC, stx2 and tsh genes were present in 9/15(60%), 7/15(46%) and of the isolates, respectively. Only one isolate gave positive amplification for stx1 and ibeA genes each. The hly gene was not encountered in any of the tested isolates.

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Virulence Factors of Avian Pathogenic Escherichia coli Isolated from Broilers from the South of Brazil

Cited by 16 publications

References 23 publications

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Genes associated with pathogenicity of avian Escherichia coli (APEC) isolated from respiratory cases of poultry

Molecular Screening Of Virulence Genes In Avian Pathogenic Esherichia Coli

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