Virulence factors expressed by Mycobacterium ulcerans strains: Results of a descriptive study

guetta, Aka N; Solange, Kakou-N’Gazoa; Coulibaly, N D; Sabine, Vakou N’dri; Kouadio, Kouamé; Aoussi, Serge; Constance, Kadio M.; Helene, Kouakou; Aké, Julien; Raymond, N Guessan; Dosso, Mireille

doi:10.5897/ajmr2015.7393

Cited by 2 publications

(1 citation statement)

References 30 publications

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“…In Côte d'Ivoire, studies on mycobacteriosis have focused more on Mycobacterium ulcerans, which is responsible for Buruli ulcer and Mycobacterium Tuberculosis for respiratory's infection [3,4]. NTMs can cause certain diseases like pneumonia, lung abscess, pleural infection, meningitis, lymphadenitis and many infections of the skin and soft tissue.…”

Section: Introductionmentioning

confidence: 99%

First Detection of Mycobacterium sp. in Wastewater in Abidjan (Yopougon) Cote d’Ivoire

Sabine

Julien

Nina

et al. 2020

SAJRM

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Non-tuberculous mycobacteria (NTM) are ubiquitous in the environment and worldwide, under certain environmental conditions, they could be responsible for skin diseases or pulmonary lesions. In Ivory Coast, Buruli ulcers endemic country, very little information concerning the epidemiology of NTM in Cote d'Ivoire is known, this ignorance has led to an underestimation of its prevalence in the environment. Aims: The objective of this study was to characterize strains of environmental mycobacteria in wastewater located in urban areas in Abidjan (Yopougon) Côte d'Ivoire. Place and Duration of Study: The study was conducted at Yopougon (Selmer). This site is considered to be Buruli ulcer non-endemic zone according to the national program on fight against Buruli ulcer in Côte d’Ivoire. However, all biological aspect of this work was carried out at the Pastor Institute of Côte d’Ivoire. Methodology: Wastewater samples were collected at the surfaces at Yopougon using the technique described by Britton and Gresson. In the laboratory, these collected samples were treated according to the method described by Stinear et al. and Kankya et al. consisting of decontamination, neutralization followed by culture. Classification of species was done according to the method described by Runyon and collaborators. Biochemical identification of mycobacteria was done according to the method described by Metchock. Results: A total of 32 samples were collected, 8 water samples at each sampling site. After decontamination and culture, specimens grew on Lowenstein-Jensen medium after 7 days (at 37°C and 32°C) and revealed small, smooth, nonpigmented colonies. Smears confirmed the presence of variably acid-fast organisms. Biochemical traits of the isolates revealed, colony grown on Middlebrook agar 7H11 (at 37°C and 32°C); catalase (+); agar (+) (at 37°C and 32°C ); citrate (-); mannitol (-). Conclusion: This work is one of the first study focusing on Mycobacteria in wastewater in Abidjan. The isolation of Mycobacterium sp., an environmental pathogenic organism, supports the hypothesis of possible colonization of urban wastewater by pathogenic mycobacteria. Further studies are needed to verify the involvement of this specie in mycobacterial infections in Côte d'Ivoire.

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Section: Introductionmentioning

confidence: 99%

First Detection of Mycobacterium sp. in Wastewater in Abidjan (Yopougon) Cote d’Ivoire

Sabine

Julien

Nina

et al. 2020

SAJRM

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Microbiological and molecular characterization of environmental mycobacterium strains isolated from the Buruli ulcer endemic and non-endemic zones in Cte dIvoire

Vakou¹,

Julien²,

Kakou-Ngazoa³

et al. 2016

Afr. J. Microbiol. Res.

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Mycobacterium ulcerans (MU), the causative agent of Buruli ulcer (BU), skin disease, is considered to be an environmental pathogen. The pathogenic virulence of MU is being linked to the expression of toxin called Mycolactone. Genetic analyses have shown the high diversity with variable number tandem repeats (VNTR) and mycobacterial interspersed repetitive units (MIRU) in M. ulcerans and in mycolactone producing Mycobacteria (MPMs). The purpose of this study is the molecular characterization of potentially pathogenic environmental mycobacteria strain, apart from the M. ulcerans, from aquatic environments in Côte d'Ivoire. A total of 473 samples were collected comprising of 251 water and 222 sediment based on sampling sites. The sediments were the most contaminated by mycobacteria with 60% as against 43.3% in water samples from the hyper endemic areas. In hypoendemic areas, water was the most contaminated with 53.57% against 43.24% in sediment. Microscopy by Ziehl-Neelsen-staining, and PCR diagnostics using IS2404 and KR were performed on strains. 20% fast growing isolated mycobacteria species including Mycobacterium mucogenicum, Mycobacterium peregrinum and Mycobacterium sp. was found carrying the IS2404 gene previously found in M. ulcerans. 9.23% of strains carry the ketoreductase (KR) genes, one of the synthesis of mycolactone enzymes. In terms of genetic analysis using the MIRU/VNTR, the MIRU 1 was the most amplified sequence, and LOCUS 6 less amplified; no known profile have been identified in this study. This study is the first step taken in order to understand different skin infections encountered in Côte d'Ivoire.

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Virulence factors expressed by Mycobacterium ulcerans strains: Results of a descriptive study

Cited by 2 publications

References 30 publications

First Detection of Mycobacterium sp. in Wastewater in Abidjan (Yopougon) Cote d’Ivoire

First Detection of Mycobacterium sp. in Wastewater in Abidjan (Yopougon) Cote d’Ivoire

Microbiological and molecular characterization of environmental mycobacterium strains isolated from the Buruli ulcer endemic and non-endemic zones in Cte dIvoire

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