Virulence factors, antibiotic resistance phenotypes and O-serogroups of Escherichia coli strains isolated from community-acquired urinary tract infection patients in Mexico

Paniagua-Contreras, Gloria Luz; Monroy-Pérez, Eric; Rodríguez-Moctezuma, José Raymundo; Domínguez-Trejo, Pablo; Vaca-Paniagua, Felipe; Vaca, Sergio

doi:10.1016/j.jmii.2015.08.005

Cited by 73 publications

(80 citation statements)

References 33 publications

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“…The DNA of the strains was extracted by boiling, as described by Paniagua-Contreras et al [15]. The C. albicans strain B311 (ATCC 32354, ATCC, Manassas, VA, USA) was used as a positive control.…”

Section: Methodsmentioning

confidence: 99%

High Virulence and Antifungal Resistance in Clinical Strains ofCandida albicans

Monroy-Pérez

Paniagua-Contreras

Rodríguez-Purata

et al. 2016

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Antifungal resistance and virulence properties of Candida albicans are a growing health problem worldwide. To study the expression of virulence and azole resistance genes in 39 clinical strains of C. albicans, we used a model of infection of human vaginal epithelial cells with C. albicans strains isolated from Mexican women with vulvovaginal candidiasis (VVC). The strains were identified by PCR amplification of the ITS1 and ITS2 regions of rRNA. The detection and expression of virulence genes and azole resistance genes MDR1 and CDR1 were performed using PCR and RT-PCR, respectively. All strains were sensitive to nystatin and 38 (97.4%) and 37 (94.9%) were resistant to ketoconazole and fluconazole, respectively. ALS1, SAP4–SAP6, LIP1, LIP2, LIP4, LIP6, LIP7, LIP9, LIP10, and PLB1-PLB2 were present in all strains; SAP1 was identified in 37 (94.8%) isolates, HWP1 in 35 (89.7%), ALS3 in 14 (35.8%), and CDR1 in 26 (66.6%). In nearly all of the strains, ALS1, HWP1, SAP4–SAP6, LIP1–LIP10, PLB1, and PLB2 were expressed, whereas CDR1 was expressed in 20 (51.3%) and ALS3 in 14 (35.8%). In our in vitro model of infection with C. albicans, the clinical strains showed different expression profiles of virulence genes in association with the azole resistance gene CDR1. The results indicate that the strains that infect Mexican patients suffering from VVC are highly virulent and virtually all are insensitive to azoles.

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Section: Methodsmentioning

confidence: 99%

High Virulence and Antifungal Resistance in Clinical Strains ofCandida albicans

Monroy-Pérez

Paniagua-Contreras

Rodríguez-Purata

et al. 2016

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…The emergence of virulence is mainly due to the presence of multiple virulence genes in E. coli pathogenicity islands. fyuA is highly pathogenic and is often used as an indication of the presence or absence of high pathogenicity islands (HPI; Paniagua et al, 2017).…”

Section: Antimicrobial Resistance Genotypes Of E Coli Isolatesmentioning

confidence: 99%

“…Bacterial pili and fimbriae are important structures for bacterial pathogenicity, and it has been suggested that type I fimbriae function primarily in the initial pathogenic phase of avian pathogenic E. coli (APEC) infection. P-type fimbriae are also thought to contribute to bacterial pathogenicity (Paniagua et al, 2017). The fimC virulence gene encodes a protein necessary for the biosynthesis of type I fimbriae.…”

Section: Virulence Genes Of E Coli Isolatesmentioning

confidence: 99%

Antimicrobial resistance of Escherichia coli isolated from retail foods in northern Xinjiang, China

Zhang

Luo

et al. 2020

Food Science & Nutrition

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To determine antimicrobial resistance, 431 samples of retail foods purchased at different supermarkets in Northern Xinjiang were examined in this study. There were 112 Escherichia coli strains that were isolated, with approximately 26% of the samples contaminated by E. coli. The detection rate of E. coli isolated from pork was the highest (59.6%), followed by mutton (52.6%), retail fresh milk (52.4%), duck (36.4%), beef (35.3%), chicken (33.3%), and ready-to-eat food (12.9%); the E. coli detection rate for fish and vegetables was <11%. The result showed that the 112 isolates were mostly resistant to tetracycline (52%), followed by ampicillin (42%), compound trimethoprim/sulfamethoxazole (37%), amoxicillin (33%), and nalidixic acid (32%), imipenem resistance was not detected. One hundred isolates carried at least one antimicrobial resistance gene. The detection rate of resistance genes of our study was as follows:tetA (38%), tetB (27%), bla OXA (40%), bla TEM (20%), floR (20%), sul1 (16%), sul2 (27%), aad Ala (19%), aadB (11%), strA (28%), and strB (24%); tetC and bla PSE were not detected. Virulence genes fimC, agg, stx2, fimA, fyuA, papA, stx1, and eaeA were found in 52, 34, 21, 19, 6, 3, 2, and 2 isolates, respectively; papC was not detected. There was a statistically significant association between fimC and resistance to ciprofloxacin (p = .001), gentamicin (p = .001), amikacin (p = .001), levofloxacin (p = .001), and streptomycin (p = .001); between fimA and resistance to tetracycline (p = .001), ampicillin (p = .001), compound trimethoprim/sulfamethoxazole (p = .001), and amoxicillin (p = .003); between agg and resistance to gentamicin (p = .001), tetracycline (p = .001), ciprofloxacin (p = .017), and levofloxacin (p = .001); and between stx2 and resistance to ampicillin (p = .001), tetracycline (p = .001), compound trimethoprim/ sulfamethoxazole (p = .002), and amoxicillin (p = .015).

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“…Urinary tract infections (UTIs) are one of the most common infections, affecting both outpatients and inpatients worldwide 1 . Uropathogenic Escherichia coli (UPEC), classified as Extraintestinal pathogenic E. coli (ExPEC), are one of the most predominant causes of UTIs 2 .…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Group/Subgroups Distributions, Virulence Factors, and Antimicrobial Susceptibility of Escherichia coli Strains from Urinary Tract Infections in Hatay

Yılmaz

Aslantaş

2020

Rev. Soc. Bras. Med. Trop.

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Introduction: Nosocomial and community acquired urinary tract infections (UTIs) are one of the most encountered infections in the world. Methods: This study aimed to determine the antibiotic susceptibility, phylogeny, and virulence genes of 153 Escherichia coli strains isolated from UTIs. Antimicrobial susceptibility of the isolates to different classes of antimicrobials was determined by the VITEK-2 automated system. Presence of virulence genes and phylogenetic groups were investigated by PCR. Results: Regarding susceptibility to antimicrobials, ampicillin resistance was most abundant (67.3%), followed by amoxicillin-clavulanic acid (50.9%); least abundant was resistance to amikacin (1.3%) and nitrofurantoin (1.3%). Multi drug resistance (MDR) was observed in 34.6% of the isolates, and all isolates were found to be susceptible to imipenem, meropenem and fosfomycine. The majority of the isolates belonged to the phylogenetic group B2 3 (35.9%), followed by A1 (20.9%), D1 (18.9%), D2 (12.4%), A0 (%5.9), B1 (3.9%) and B2 (1.9%). Among E. coli strains examined, 49% had iucD, 32.7% papE-F, 26.1% papC, 15% cnf2, 11.1% sfa, 7.8% cnf1, 1.3% afaE, 1.3% afaD, 1.3% hlyA, 0.7% f17a-A, 0.7% clpG and 0.7% eaeA genes. Conclusions: Our research demonstrated that virulence factors were distributed among different phylogroup/subgroups, which play a role in UTIs pathogenesis in humans. For this reason, complex and detailed studies are required to determine the relationship between virulence factors and specific E. coli strains that cause UTIs in humans. FIGURE 1: PCR amplification of E. coli specific 16S rRNA gene (401 bp).

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Virulence factors, antibiotic resistance phenotypes and O-serogroups of Escherichia coli strains isolated from community-acquired urinary tract infection patients in Mexico

Abstract: The isolated UPEC strains predominantly belonged to three serogroups (O25, O15, and O8), harboured numerous virulence genes, and are multiresistant to antibiotics. The findings of this study could be used to orient UTI treatment strategies and in epidemiological studies in Mexico.

Cited by 73 publications

References 33 publications

High Virulence and Antifungal Resistance in Clinical Strains ofCandida albicans

High Virulence and Antifungal Resistance in Clinical Strains ofCandida albicans

Antimicrobial resistance of Escherichia coli isolated from retail foods in northern Xinjiang, China

Phylogenetic Group/Subgroups Distributions, Virulence Factors, and Antimicrobial Susceptibility of Escherichia coli Strains from Urinary Tract Infections in Hatay

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