Virulence factors and antimicrobial resistance in uropathogenic &lt;i&gt;Escherichia&lt;/i&gt; &lt;i&gt;coli&lt;/i&gt; strains isolated from cystitis and pyelonephritis

GHAZVINI, Hosseinali; Taheri, Keyvan; Edalati, Elahe; Sedighi, Mansour

doi:10.3906/sag-1805-100

Cited by 21 publications

(23 citation statements)

References 33 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of our current report is in concordance with that conducted by Shashwati and his associates, who studied the prevalence of ESBLs among the patients they attended at the tertiary care center; their results showed that ESBL producers were more in males (52.54%) than in females [21]. Findings of the present study and previous ones confirmed that male gender is one of the risk factors associated with community-acquired ESBL-positive UTI [22,23].…”

Section: Discussionsupporting

confidence: 92%

Determination of the frequency of β-lactamase genes (bla SHV, bla TEM, bla CTX-M) and phylogenetic groups among ESBL-producing uropathogenic Escherichia coli isolated from outpatients

Mirkalantari

Masjedian

Irajian

et al. 2019

Journal of Laboratory Medicine

View full text Add to dashboard Cite

Background Escherichia coli accounts for 70–95% of community-acquired urinary tract infections (UTIs). Recently, there has been an increase in the prevalence of extended-spectrum β-lactamase (ESBL) in the community which required an accurate identification for better management. Therefore, the current study was performed to determine the antimicrobial resistance pattern, investigate ESBL phenotypes and genotypes (blaCTX-M, bla TEM and bla SHV genes) and determine the phylogenetic groups among ESBL-positive isolates from outpatients. Methods One hundred and eighty-three positive urine samples were collected from 4450 outpatient clinic attendees. Antibiotic susceptibility was determined and ESBL phenotype screening was carried out using disk diffusion agar and combination disk techniques, respectively. The assessment of the presence of the blaCTX-M, bla TEM and blaSHV genes and phylogenetic grouping were performed using the polymerase chain reaction (PCR) method. Results Out of 183 E. coli isolates, 59 (32.2%) showed a positive ESBL phenotype. The prevalence of ESBL-producing E. coli was higher in males (57.4%). Fifty-seven of the ESBL-producing strains carried at least one of the β-lactamase genes (bla CTX-M, bla TEM, bla SHV). Phylotyping of multi-drug resistant isolates indicated that the isolates belonged to B2, A and D phylogroups. Analysis of resistance patterns among these phylogroups revealed that 74.4%, 55.3% and 29.7% of the isolates in the B2 group were resistant to trimethoprim-sulfamethoxazole, ciprofloxacin and gentamicin, respectively. Most of the strains in the phylogroup B2 carried the bla CTX-M gene. Conclusions All the ESBL-producing isolates were placed in one of the four phylogenetic groups. The presence of CTX-M and resistance to quinolones were more frequent in B2 strains than in non-B2 strains.

show abstract

Section: Discussionsupporting

confidence: 92%

Determination of the frequency of β-lactamase genes (bla SHV, bla TEM, bla CTX-M) and phylogenetic groups among ESBL-producing uropathogenic Escherichia coli isolated from outpatients

Mirkalantari

Masjedian

Irajian

et al. 2019

Journal of Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…A similar observation was reported in Iran by (Tabasi et al, 2015). In the developing world where apart from high level of poverty and ignorance, there is also a widespread of antibiotics misuse, in addition to, their low-cost easy availability in the community without prescription which make patients with symptomatic UTI subject to abuse then result in increasing the drug resistance (Abubakar, 2009).The current work observed that fimH (the adhesive subunit of type 1 fimbriae of UPEC) was the most prevalent virulence factor detected in UPEC strains and presented with the highest frequency (92.6%) in urine isolates as compared to the rest of the genes detected , this is similar to studies performed in Iran by (Asadi et al, 2014 andGhazvin et al, 2019) and in Sudan by (Hassan et al, 2018). This reconfirms the crucial and essential role of fimH gene in E. coli to cause UTI among pregnant females, that is because of the higher binding ability of fimH which could result in the increased pathogenicity of UPEC strains as most strains of (UPEC) encode filamentous adhesive organelles called type 1 pili which is important for the invasion, adherence and persistence of the UPEC in the urinary bladder after its colonization which enhanced by adhesin FimH (Hannan et al, 2012).…”

Section: Discussionsupporting

confidence: 87%

Genotypic Detection of the Virulence Factors of Uropathogenic Escherichia Coli (Upec) Strains Isolated From Pregnant Females and Their Correlation With Antibiotic Resistance Pattern

Ahmed

2021

Al-Azhar Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

Urinary Tract Infections (UTIs) during pregnancy are among the most common infections worldwide and can lead to poor perinatal and maternal outcomes, especially in developing countries and requires medical treatment as soon as it is detected. Virulent and resistant strains of Escherichia coli are the most important causative agents for UTIs. The present investigation aimed to detect virulence factors of Uropathogenic Escherichia coli (UPEC), including pap, fim, sfa, aer and hly genes in isolates collected from symptomatic and asymptomatic pregnant females by multiplex polymerase chain reaction (PCR) assay and determine their correlation with antibiotic resistance patterns. Two hundred urine samples collected from pregnant females with or without symptoms of UTI were admitted to antenatal clinic at Obstetrics and Gynecology Department of Al-Zahraa University Hospital in Cairo. Out of 200 cultured urine samples, the cultures that showed ≥ 10 5 CFU/ml were identified to have significant growth. Found significant bacteriuria was detected in 61 samples and these samples selected for further analysis in this study, the overall prevalence of UTI among pregnant females was (30.5%). Symptomatic pregnant females with UTI were (48.5%) more than asymptomatic (11.3%). The most frequently isolated species was S. saprophyticus (35.0%), followed by E. coli (26.2%), S .aureus (19.4%), C .albicans (5.8%), S. epidermidis (2.9%) and each of K. pneumonia, K .oxytoca, P .vulgaris, E.fecalis, Bacillus spp were (1.9%), while P. auroginosa had the least percentage of isolation (1.0%). Antimicrobial susceptibility pattern was done for 27 UPEC isolates showed the highest level of resistance (100%) against β-lactams as (Ampicillin, Penicillin and Ceftriaxone), nitrofurantoin, and ampicillin/sulbactam, while the lowest level of resistance (66.7%) against Cotrimoxazole. Multiple drug resistance (resistance to two or more drugs) was observed in (100%) of the UPEC isolates. The higher level of resistance to antibiotics was observed in symptomatic bacteriuria more than asymptomatic. Among two predominant uropathogens, E. coli showed complete resistance up to 5 different antibiotics of total 11 antibiotics (45.5%) while S. saprophyticus showed complete resistance (18.2%), so E. coli showed higher virulence and resistance than S. saprophyticus to cause UTI, so subjected to Multiplex PCR to detect its virulence factors, which included pap, fim, sfa, aer and hly genes The adhesive fimH gene was the most frequent in UPEC isolates (92.6%). These virulence genes detected from total UPEC isolates of symptomatic cases were higher than asymptomatic. On the other hand, UPEC strains carrying the virulence genes were more resistant to the antibiotics used, so these observations confirm the important role of virulence genes of UPEC in in existence of symptoms of UTI and the drug resistance.

show abstract

“…Among 12 studies which investigated the prevalence of fimH , 10 studies reported a high frequency of 64% to 100% for fimH . Only Hassan et al and Bahalo et al reported a low prevalence of 28% and 30% for fimH , respectively ( 14 , 19 , 22 – 32 ). In this study, most of the isolates carried fimH as fimbria factor; uropathogenic E. coli isolates, which cause a wide range of human UTIs, and infection following bacterial adhesion in which catch-bonds are significant.…”

Section: Discussionmentioning

confidence: 95%

Virulence factors analysis and antibiotic resistance of uropathogenic Escherichia coli isolated from patients in northeast of Iran

Ghavidel¹,

Gholamhosseini-Moghadam²,

Nourian³

et al. 2020

IJM

View full text Add to dashboard Cite

Background and Objectives: Escherichia coli is known to be the pathogen commonly isolated from those infected with uri- nary tract infections (UTIs). The aim of this study was to investigate the presence of E. coli virulence genes and antibiotics’ resistance pattern among clinical isolates in the Northeast of Iran. Relationships between virulence genes and antimicrobial resistances were studied as well. Materials and Methods: Three hundred isolates of E. coli were isolated from patients with UTIs that referred to Ghaem and Imam Reza hospitals (Mashhad, Iran) during August 2016 to February 2017. A multiplex PCR was employed to amplify the genes encoding pyelonephritis associated pili (pap), S-family adhesions (sfa), type1fimbriae (fimH) and aerobactin (aer). Disk diffusion test was performed to test the susceptibility of isolates to β-lactams, aminoglycosides, cephalosporins, quino- lone, fluoroquinolones, carbapenems and trimethoprim-sulfamethoxazole. Results: The PCR results identified the fimH in 78.4%, aer in 70.5%, sfa in 13.6% and the pap in 8.2% of isolates. The rates of antibiotic resistance of the isolates were as follows: 64.7% resistant to cephalosporins, 34% to trimethoprim-sul- famethoxazole, 31% to fluoroquinolones, 15.3% to aminoglycosides, 13.3% to β-lactams, 7.8% to quinolones and 4.4% to carbapenems. Significant relationships existed between pap and aer, pap and sfa, aer and fluoroquinolones also pap and cephalosporins. Conclusion: fimH and aer were found in > 50% of isolates suggesting the importance of both genes in UPEC. The majority of isolates had fimH as adhesion factor for colonization. Determining antibiotic resistance patterns in specific geographical areas is necessary for appropriate treatment of urinary tract infection. The high rate of resistance to cephalosporins is most likely due to incorrect drug administration

show abstract

Virulence factors and antimicrobial resistance in uropathogenic <i>Escherichia</i> <i>coli</i> strains isolated from cystitis and pyelonephritis

Cited by 21 publications

References 33 publications

Determination of the frequency of β-lactamase genes (bla SHV, bla TEM, bla CTX-M) and phylogenetic groups among ESBL-producing uropathogenic Escherichia coli isolated from outpatients

Determination of the frequency of β-lactamase genes (bla SHV, bla TEM, bla CTX-M) and phylogenetic groups among ESBL-producing uropathogenic Escherichia coli isolated from outpatients

Genotypic Detection of the Virulence Factors of Uropathogenic Escherichia Coli (Upec) Strains Isolated From Pregnant Females and Their Correlation With Antibiotic Resistance Pattern

Virulence factors analysis and antibiotic resistance of uropathogenic Escherichia coli isolated from patients in northeast of Iran

Contact Info

Product

Resources

About