Virulence factors and antibiotic resistance patterns of uropathogenic Escherichia coli

Mbanga, Joshua; Mudzana, Raymond

doi:10.5897/ajmr2014.7034

Cited by 9 publications

(9 citation statements)

References 29 publications

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“…In this study 103 (51.5%) uropathogenic E. coli isolates carries hemolysin (hly) gene which is comparable to studies conducted in Iran, 50.4% [28] and South Korea, 62% [39]; but higher than studies conducted in Zimbabwe, 12.5% [14]; Tunisia, 19% [12]; Poland, 18.5% [42]; Mexico, 15.4% [31] and China, 11.6% [30].In our study, hemolysin gene was significantly associated with suprapubic pain (p-0.002). This indicates that hemolysin may be responsible for clinical manifestation in UTI patients.…”

Section: Discussionsupporting

confidence: 57%

“…The genetic determinants that are studied includes those coding for type 1 [12,[14][15][16].Specific primers were used to amplify sequences of the fim, pap, sfa/foc, afa, hly, cnf, and aer operons. Details of primer sequences and predicted sizes of the amplified products are given in Table 1.…”

Section: Detection Of Virulence Genes Of Uropathogenic Escherichia Colimentioning

confidence: 99%

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Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

et al. 2020

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Background: Urinary tract infection (UTI) is a common cause of morbidity worldwide. Uropathogenic Escherichia coli (UPEC) bacteria are the major cause of urinary tract infections. UPEC strains derive from different phylogenetic groups and possess an arsenal of virulence factors that contribute to their ability to overcome different defense mechanisms and cause disease. The objective of this study was to identify phylogroup and virulence genes of UPEC among urinary tract infection patients. Methods: A cross sectional study was conducted from January 1, 2017 to October 9, 2017. E. coli bacteria were isolated from UTI patients using culture and conventional biochemical tests. Identification of phylogroup and genes that encodes for virulence factors was done using multiplex polymerase chain reaction (PCR). Data was processed and analyzed with SPSS version16.0 and Epi-info version 3.4.1 software. Results: The most common urologic clinical manifestation combinations in this study were dysuria, urine urgency and urgency incontinence. The frequent UPEC virulence gene identified was fimH 164 (82%), followed by aer 109 (54.5%), hly 103 (51.5%), pap 59 (29.5%), cnf 58 (29%), sfa 50 (25%) and afa 24 (12%).There was significant association between pap gene and urine urgency (p-0.016); sfa and dysuria and urine urgency (p-0.019 and p-0.043 respectively); hly and suprapubic pain (p-0.002); aer and suprapubic pain, flank pain and fever (p-0.017, p-0.040, p-0.029 respectively). Majority of E. coli isolates were phylogroup B2 60(30%) followed by D 55(27.5%), B1 48(24%) and A 37(18.5%). There was significant association between E. coli phylogroup B2 and three virulence genes namely afa, pap, and sfa (p-0.014, p-0.002, p-0.004 respectively). Conclusion: In this study the most frequent E. coli virulence gene was fimH, followed by aer, hly, pap, cnf, sfa and afa respectively. There was significant association between E. coli virulence genes and clinical symptoms of UTI. The phylogenetic analysis indicates majority of uropathogenic E. coli isolates were phylogroup B2 followed by phylogroup D. Phylogroup B2 carries more virulence genes. Hence, targeting major UPEC phylogroup and virulence genes for potential vaccine candidates is essential for better management of UTI and further research has to be conducted in this area.

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Section: Discussionsupporting

confidence: 57%

Section: Detection Of Virulence Genes Of Uropathogenic Escherichia Colimentioning

confidence: 99%

Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

et al. 2020

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“…Moreover, Hemolysin production could be linked to high prevalence of ESBL production and drug resistance [40]. Occurrence of high numbers of Hemolysin producers is in consistence with the work of other workers; Bhrugubalda et al Mittal et al Mbanga and Mudzana; Sharma and Sabitha et al [10,19,29,38,39]. In this study it is also a noteworthy observation that few isolates from healthy children were also Hemolysin producers (n=20; 10%).…”

Section: Antibiotic Susceptibility Findingssupporting

confidence: 72%

“…Misuse of antibiotics in human practice, animal husbandry systems and agriculture has contributed to a large extent on development of drug resistance. Multidrug resistance, MDR, exhibited by resistance to three or more than three classes of antibiotics [28], is a common phenomenon among Enterobacteriaceae [29,19].…”

Section: Introductionmentioning

confidence: 99%

Phenotypic Characterisation of Escherichia coli Isolates from Fish, Diarrheic and Healthy Children in Zanzibar, Tanzania

Rabia

Wambura

Kimera

et al. 2017

IJTDH

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and fish consumers through the seasons. Cross sectional design was used to collect children faecal samples from Mnazimmoja referral hospital. Place and Duration of Study: The study was carried out in Zanzibar between August 2014 and June 2016 Methodology: A total of 113 E. coli isolates from fish (58), diarrheic (35) and healthy children (20) less than five years old were screened. Serotyping was used for detection of serotype O157 and extended-spectrum-β-lactamases (ESBL) production by disc diffusion using Cepodoxime/clavulanic acid (10/1μg) discs. Hemolysin was detected by hemolysis in human blood agar, serum resistance factors by growth inhibition of E. coli that was mixed and incubated with human sera, Hemagglutinins by Hemagglutination of RBCs and Gelatinase enzyme by production of clear zone of degradation of gelatine. Statistical analysis was done using Medcalc statistical software. Statistical difference in virulence factors possession data in E. coli isolates between fish, diarrheic and healthy children were subjected to analysis of variance [ANOVA], where p<0.05 was judged indicative of significant difference. Results: Virulence factors detected in their order of prevalence were hemolysins (21.2%), serum resistance (12.2%), hydrophobicity (8.8%), Hemagglutinins (4.4%), and Gelatinase (2.7%). Virulence factors were detected in 82.9% and 38% of diarrheic children and fish isolates respectively. E. coli O157 serotype was detected in all 3 sources with higher percentage in diarrheic children (6.8%). Extended spectrum β-lactamase E. coli producers were found in fish (6.8%) and diarrheic samples (17.1%) but not from healthy children. Multidrug resistance [MDR] was detected in fish (44.9%), diarrheic children (82.8%) and healthy children (10%). Ampicillin (100%; 22.4%) and Tetracycline (82.9%; 44.8%) exhibited high antibiotic resistance both in diarrheic children and fish respectively. Fish foods could be sources of pathogenic and antibiotic resistant E. coli possessing multiple virulence factors. Moreover O157 serotypes, ESBL producing and multidrug resistant E. coli were also detected in fish foods. Conclusion: It is therefore emphasised to improve hygiene in the fish value chain as well as raise awareness and frequent monitoring to fish stakeholders in Zanzibar.

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“…The genetic determinants that are studied includes those coding for type 1 (11,13,14,15).Specific primers were used to amplify sequences of the fim, pap, sfa/foc, afa, hly, cnf, and aer operons. Details of primer sequences and predicted sizes of the amplified products are given in Table 1.…”

Section: Detection Of Virulence Genes Of Uropathogenic Escherichia Colimentioning

confidence: 99%

Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

Dadi

Abebe

Zhang

et al. 2019

Preprint

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Background Urinary tract infection (UTI) is a common cause of morbidity and mortality worldwide. Uropathogenic Escherichia coli (UPEC) bacteria are the major cause of urinary tract infections. UPEC strains derive from different phylogenetic groups and possess an arsenal of virulence factors that contribute to their ability to overcome different defense mechanisms and cause disease. The objective of this study was to identify phylogroup and virulence genes of UPEC among urinary tract infection patients. Methods A total of 200 E. coli bacteria were isolated from 780 UTI patients using culture and conventional biochemical tests. Identification of phylogroup and genes that encodes for virulence factors was done using multiplex polymerase chain reaction (PCR). Data was processed and analyzed with SPSS version16.0 and Epi-info version 3.4.1 software. Result The most common urologic clinical manifestation combinations in this study were dysuria, urine urgency and urgency incontinence. The frequent UPEC virulence gene identified was fim H 164 (82%), followed by aer 109 (54.5%), hly 103 (51.5%), pap 59 (29.5%), cnf 58 (29%), sfa 50 (25%) and afa 24 (12%).There was significant association between pap gene and urine urgency (p-0.016); sfa and dysuria and urine urgency (p-0.019 and p-0.043 respectively); hly and suprapubic pain (p-0.002); aer and suprapubic pain, flank pain and fever (p-0.017, p-0.040, p-0.029 respectively). Majority of E. coli isolates were phylogroup B2 60(30%) followed by D 55(27.5%), B1 48(24%) and A 37(18.5%). There was significant association between E. coli phylogroup B2 and three virulence genes namely afa, pap, and sfa (p-0.014, p-0.002, p-0.004 respectively). Conclusion In this study the most frequent E. coli virulence gene was fim H, followed by aer, hly, pap, cnf, sfa and afa respectively. There was significant association between E. coli virulence genes and clinical symptoms of UTI. The phylogenetic analysis indicates majority of uropathogenic E. coli isolates were phylogroup B2 followed by phylogroup D. Phylogroup B2 carries more virulence genes. Hence, targeting major UPEC phylogroup and virulence genes for potential vaccine candidates is essential for better management of UTI and further research has to be conducted in this area.

show abstract

Virulence factors and antibiotic resistance patterns of uropathogenic Escherichia coli

Cited by 9 publications

References 29 publications

Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

Phenotypic Characterisation of Escherichia coli Isolates from Fish, Diarrheic and Healthy Children in Zanzibar, Tanzania

Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

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