Virulence-encoding genes related to extraintestinal pathogenic E. coli and multidrug resistant pattern of strains isolated from neonatal calves with different severity scores of umbilical infections

Martins, Lorrayne de Souza Araújo; Mott, Rodrigo G.; Martinez, Antônio C.; Orsi, Henrique; Hernandes, Rodrigo T.; Rall, Vera Lúcia Mores; Pantoja, J.C.F.; Ribeiro, Márcio Garcia

doi:10.1016/j.micpath.2022.105861

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…Akiyama et al (2015) found diarrheic genes, including astA, in several E. coli groups, such as Shiga-toxin-producing E. coli and Diarrheagenic E. coli from cattle in Japan. Recently, Martins et al (2022) found that the serum resistance-related genes (traT, ompT) could be used as biomarkers to investigate ExPEC isolates from umbilical infections in calves. Other reports showed that E. coli strains isolated from cattle in Lithuania, China, and Egypt could harbor those pathogenic genes, including fuyA, traT, and tsh.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance and Pathogenicity of Escherichia coli Isolated from Cattle Raised in Households in the Mekong Delta, Vietnam

2024

Int J Vet Sci

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Of 127 cattle feces samples collected in the Mekong Delta from March to June 2023, 121 samples were positive for E. coli (95.28%). In those surveyed households, there was no statistically significant difference in the prevalence of E. coli in cattle of different ages, dairy or beef. The antimicrobial susceptibility test indicated that those E. coli strains were susceptible to ceftazidime (87.27%), cefuroxime (89.09%), amikacin, and doxycycline (90.91%). However, E. coli strains were remarkably resistant to ampicillin (81.82%), streptomycin and tetracycline (74.55%), colistin and chloramphenicol (70.91%), amoxicillin/clav.acid (65.45%). Moreover, 63.64% of examined E. coli strains were resistant to one to twelve antibiotics, and the resistant pattern of Am + Ac + Co + Sm + Te + Cl accounted for the highest rate (16.36%). By PCR method, the presence of genes encoding antibiotic resistance was determined, in which the tetA gene had the highest presence (69.09%), and the lowest was the blaTEM gene (18.18%). Most E. coli (34.55%) strains harbored two to four antibiotic resistance genes, and the phenotypes of cat1 + sulII and sulII + tetA were the most common (5.45%). Using the PCR method to determine the presence of virulence genes, it was recorded that the tsh gene had the highest presence rate (54.55%), and the lowest was the fyuA gene (12.73%). Those E. coli strains (23.63%) could carry two to four virulence genes, and the most common pattern was astA + tsh (9.09%). Thus, the prevalence of pathogenic and antibiotic-resistant E. coli in cattle was critical to protect animal and human health.

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Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance and Pathogenicity of Escherichia coli Isolated from Cattle Raised in Households in the Mekong Delta, Vietnam

2024

Int J Vet Sci

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Identification of Escherichia coli isolated from flies (Insecta: Diptera) that inhabit the environment of dairy farms harboring extraintestinal virulence markers

Motta,

Bertolini

et al. 2023

Journal of Applied Microbiology

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Aims We investigate extraintestinal pathogenic genes (ExPEC) related to virulence of Escherichia coli in flies from the dairy environment. Methods and Results We collected 217 flies from nine dairy farms, which were submitted to microbiological culture. Fifty-one E. coli were identified using mass spectrometry. Eleven dipteran families were identified, with a predominance of Muscidae, and a minor frequency of Tachinidae, Drosophilidae, Sphaeroceridae, Ulidiidae, Syrphidae, Chloropidae, Calliphoridae, Sarcophagidae, and Piophilidae. A panel of 16 virulence-encoding genes related to ExPEC infections were investigated, which revealed predominance of serum resistance (traT, 31/51 = 60.8%; ompT, 29/51 = 56.9%), iron uptake (irp2, 17/51 = 33.3%, iucD 11/51 = 21.6%), and adhesins (papC, 6/51 = 11.8%; papA, 5/51 = 9.8%). Conclusions Our findings reveal Dipterans from milking environment carrying ExPEC virulence-encoding genes also identified in clinical bovine E. coli-induced infections.

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Virulence-encoding genes related to extraintestinal pathogenic E. coli and multidrug resistant pattern of strains isolated from neonatal calves with different severity scores of umbilical infections

Cited by 2 publications

References 27 publications

Antibiotic Resistance and Pathogenicity of Escherichia coli Isolated from Cattle Raised in Households in the Mekong Delta, Vietnam

Antibiotic Resistance and Pathogenicity of Escherichia coli Isolated from Cattle Raised in Households in the Mekong Delta, Vietnam

Identification of Escherichia coli isolated from flies (Insecta: Diptera) that inhabit the environment of dairy farms harboring extraintestinal virulence markers

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