Virulence contribution and recognition of homologs of the Verticillium dahliae effector Ave1

Boshoven, J.C.

doi:10.18174/420169

Cited by 3 publications

(4 citation statements)

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“…Verticillium dahliae, V. albo-atrum , and F. oxysporum ( Fol ) inoculations were carried out with root dipping as described previously ( Fradin et al, 2009 ; Boshoven, 2017 ). For phenotyping, stunting (%) between inoculated and mock-inoculated plants was calculated based on plant canopy area at 21 days post inoculation (dpi) using ImageJ ( Abramoff et al, 2004 ) as follows:…”

Section: Methodsmentioning

confidence: 99%

“…Pathogen Inoculations, Phenotyping, and Fungal Biomass Quantification Verticillium dahliae, V. albo-atrum, and F. oxysporum (Fol) inoculations were carried out with root dipping as described previously (Fradin et al, 2009;Boshoven, 2017). For phenotyping, stunting (%) between inoculated and mockinoculated plants was calculated based on plant canopy area at 21 days post inoculation (dpi) using ImageJ (Abramoff et al, 2004) as follows: To quantify fungal biomass, stems sections (∼ 2 cm around the cotyledons) were harvested at 21 dpi and freeze-dried for 48 h. Subsequently, material was ground, and DNA was isolated using CTAB buffer (200 mM Tris-HCl pH 7.5, 50 mM EDTA pH 8.0, 2 M NaCl, 2% CTAB).…”

Section: Generation Of Crispr-cas9 and Rnai Linesmentioning

confidence: 99%

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Impairment of Tomato WAT1 Enhances Resistance to Vascular Wilt Fungi Despite Severe Growth Defects

et al. 2021

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Verticillium dahliae is a particularly notorious vascular wilt pathogen of tomato and poses a reoccurring challenge to crop protection as limited qualitative resistance is available. Therefore, alternative approaches for crop protection are pursued. One such strategy is the impairment of disease susceptibility (S) genes, which are plant genes targeted by pathogens to promote disease development. In Arabidopsis and cotton, the Walls Are Thin 1 (WAT1) gene has shown to be a S gene for V. dahliae. In this study, we identified the tomato WAT1 homolog Solyc04g080940 (SlWAT1). Transient and stable silencing of SlWAT1, based on virus-induced gene silencing (VIGS) and RNAi, respectively, did not consistently lead to reduced V. dahliae susceptibility in tomato. However, CRISPR-Cas9 tomato mutant lines carrying targeted deletions in SlWAT1 showed significantly enhanced resistance to V. dahliae, and furthermore also to Verticillium albo-atrum and Fusarium oxysporum f. sp. lycopersici (Fol). Thus, disabling the tomato WAT1 gene resulted in broad-spectrum resistance to various vascular pathogens in tomato. Unfortunately these tomato CRISPR mutant lines suffered from severe growth defects. In order to overcome the pleiotropic effect caused by the impairment of the tomato WAT1 gene, future efforts should be devoted to identifying tomato SlWAT1 mutant alleles that do not negatively impact tomato growth and development.

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Section: Methodsmentioning

confidence: 99%

Section: Generation Of Crispr-cas9 and Rnai Linesmentioning

confidence: 99%

Impairment of Tomato WAT1 Enhances Resistance to Vascular Wilt Fungi Despite Severe Growth Defects

et al. 2021

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“…V. dahliae, V. albo-atrum and Fol inoculations were carried out with root dipping as described previously (Fradin et al, 2009;Boshoven, 2017). For phenotyping, stunting (%) between inoculated and mock-inoculated plants was calculated based on plant canopy area at 21 days post inoculation (dpi) using Image J (Abramoff et al, 2004) as follows:…”

Section: Pathogen Inoculations Phenotyping and Fungal Biomass Quantifimentioning

confidence: 99%

Adding by subtracting : Impairing tomato susceptibility genes to obtain resistance to Verticillium wilt

Hanika¹

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“…Chapter 6 describes the identification of a multiallelic VdAve1-like gene in the V. dahliae population (Boshoven, 2017) and the functional analysis of the protein encoded by one of its alleles, VdAve1L2. In this chapter, we explored the genomic context of the VdAve1-like gene, as well as a process that could potentially have driven its diversification in the V. dahliae population.…”

Section: Scope and Outline Of This Thesismentioning

confidence: 99%

Functional analysis of effector proteins of the vascular wilt fungus Verticillium dahliae

Fiorin

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In order to establish disease, plant pathogens exploit effectors as molecular tools that manipulate host processes to support host colonization. Verticillium dahliae is a soil-borne fungal pathogen that causes vascular wilt disease in hundreds of dicotyledonous species. For particular hosts, V. dahliae isolates have been grouped in pathotypes based on differential aggressiveness. Based on their disease phenotypes, V. dahliae strains are typically assigned to the defoliating or the non-defoliating pathotype on cotton. A single V. dahliae effector, named the D effector, was previously demonstrated to be necessary and sufficient for the induction of defoliation by strains of the defoliating pathotype, and to act as pathogenicity determinant on this host, as well as on the model plant Arabidopsis thaliana. Thus far, the mechanism underlying D effector activity remains unknown. In this study, we used heterologously produced effector protein to investigate biochemical properties of the D effector in planta. Effector infiltration in cotton cotyledons induced yellowing, vein browning as well as necrosis, and transcript profiling showed that photosynthesis was strongly repressed, while secondary metabolism and response to stress were induced in infiltrated cotyledons. These observations indicate that the D effector triggers stress responses in cotton. In addition, we examined the effect of the D protein in cotton tissues at cellular level. Cells exhibiting reticulate cell wall reinforcements typical of tracheary elements were observed adjacent to xylem vessels and even on the epidermis, suggesting that the D effector induces ectopic xylem differentiation.

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Virulence contribution and recognition of homologs of the Verticillium dahliae effector Ave1

Abstract: A eukaryotic-acquired gene by a biotrophic phytopathogen allows prolonged survival on the host by counteracting the shutdown of plant photosynthesis. PLoS One 5:e8950.

Cited by 3 publications

References 250 publications

Impairment of Tomato WAT1 Enhances Resistance to Vascular Wilt Fungi Despite Severe Growth Defects

Impairment of Tomato WAT1 Enhances Resistance to Vascular Wilt Fungi Despite Severe Growth Defects

Adding by subtracting : Impairing tomato susceptibility genes to obtain resistance to Verticillium wilt

Functional analysis of effector proteins of the vascular wilt fungus Verticillium dahliae

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