Virulence characteristics and epidemiology of Yersinia enterocolitica and Yersiniae other than Y. pseudotuberculosis and Y. pestis isolated from water and sewage

Falcão, Juliana Pfrimer; Brocchi, Marcelo; Proença-Módena, José Luiz; Acrani, Gustavo Olszanski; Corrêa, Ednéia F.; Falcão, Deise Pasetto

doi:10.1111/j.1365-2672.2004.02268.x

Cited by 55 publications

(49 citation statements)

References 29 publications

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“…The presence of tc-like genes in Y. frederiksenii is intriguing, since the species is closely related to Yersinia enterocolitica but is generally considered nonpathogenic (9). It has most often been isolated from fresh water or sewage and only rarely from soil.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of sep Insecticidal Toxin Complex Genes in Serratia spp. and Yersinia frederiksenii

Dodd

Hurst

Glare

et al. 2006

Appl Environ Microbiol

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Some strains of Serratia entomophila and S. proteamaculans cause amber disease of the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae). Three genes required for virulence, sepABC, are located on a large plasmid, pADAP. Sequence analysis suggests that the sepABC gene cluster may be part of a horizontally mobile region. This study presents evidence for the putative mobility of the sep genes of pADAP. Southern blot analysis showed that orthologues of the sep genes reside on plasmids within S. entomophila, S. liquefaciens, S. proteamaculans, and a plasmid from Yersinia frederiksenii. Three plasmids hybridized to the pADAP sep virulenceassociated region but not the pADAP replication and conjugation regions. Subsequent DNA sequence analysis of the Y. frederiksenii sep-like genes, designated tcYF1 and tcYF2, showed that they had 88% and 87% DNA identity to sepA and sepB, respectively. These results indicate that the sep genes are part of a discrete horizontally mobile region.

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Section: Discussionmentioning

confidence: 99%

Occurrence of sep Insecticidal Toxin Complex Genes in Serratia spp. and Yersinia frederiksenii

Dodd

Hurst

Glare

et al. 2006

Appl Environ Microbiol

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“…In most cases, non-pathogenic Yersinia strains were more frequently isolated, including Yersinia frederiksenii, Y. intermedia, Yersinia kristensenii and non-pathogenic Y. enterocolitica subtypes. Pathogenic strains have been isolated from water in only a small number of studies (Fukushima et al 1984;Sandery et al 1996;Falcã o et al 2004). Fredriksson- Ahomaa et al 2006).…”

Section: Introductionmentioning

confidence: 99%

The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes

Cheyne

Dyke

Anderson

et al. 2009

Journal of Water and Health

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Yersinia enterocolitica has been detected in surface water, and drinking untreated water is a risk factor for infection. PCR-based methods have been used to detect Y. enterocolitica in various sample types, but quantitative studies have not been conducted in water. In this study, quantitative PCR (qPCR)-based methods targeting the Yersinia virulence genes ail and yadA were used to survey the Grand River watershed in southern Ontario, Canada. Initial testing of reference strains showed that ail and yadA PCR assays were specific for pathogenic biotypes of Y. enterocolitica; however the genes were also detected in one clinical Yersinia intermedia isolate. A survey of surface water from the Grand River watershed showed that both genes were detected at five sampling locations, with the ail and yadA genes detected in 38 and 21% of samples, respectively. Both genes were detected more frequently at colder water temperatures.A screening of Yersinia strains isolated from the watershed showed that the ail gene was detected in three Y. enterocolitica 1A/O:5 isolates. Results of this study show that Yersinia virulence genes were commonly detected in a watershed used as a source of drinking water, and that the occurrence of these genes was seasonal.

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“…Yersinia enterocolitica and free-living protozoa share the same ecological niches, such as water (26,27), vegetables (28,29), and anthropogenic environments, e.g., domestic refrigerators (13,30). The role of free-living protozoa in the ecology and epidemiology of food-borne pathogens such as Campylobacter, Salmonella, and Escherichia coli has already been documented (31)(32)(33), but for Y. enterocolitica, only brief descriptions of intraprotozoan replication, probably followed by the killing and digestion of the bacterial strain (34), and increased resistance to free chlorine in their early intraprotozoan lifestyle (19) have been reported so far.…”

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confidence: 99%

Behavior of Yersinia enterocolitica in the Presence of the Bacterivorous Acanthamoeba castellanii

Lambrecht

Baré

Damme

et al. 2013

Appl Environ Microbiol

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c Free-living protozoa play an important role in the ecology and epidemiology of human-pathogenic bacteria. In the present study, the interaction between Yersinia enterocolitica, an important food-borne pathogen, and the free-living amoeba Acanthamoeba castellanii was studied. Several cocultivation assays were set up to assess the resistance of Y. enterocolitica to A. castellanii predation and the impact of environmental factors and bacterial strain-specific characteristics. Results showed that all Y. enterocolitica strains persist in association with A. castellanii for at least 14 days, and associations with A. castellanii enhanced survival of Yersinia under nutrient-rich conditions at 25°C and under nutrient-poor conditions at 37°C. Amoebae cultivated in the supernatant of one Yersinia strain showed temperature-and time-dependent permeabilization. Intraprotozoan survival of Y. enterocolitica depended on nutrient availability and temperature, with up to 2.8 log CFU/ml bacteria displaying intracellular survival at 7°C for at least 4 days in nutrient-rich medium. Transmission electron microscopy was performed to locate the Yersinia cells inside the amoebae. As Yersinia and Acanthamoeba share similar ecological niches, this interaction identifies a role of free-living protozoa in the ecology and epidemiology of Y. enterocolitica.

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Virulence characteristics and epidemiology of Yersinia enterocolitica and Yersiniae other than Y. pseudotuberculosis and Y. pestis isolated from water and sewage

Cited by 55 publications

References 29 publications

Occurrence of sep Insecticidal Toxin Complex Genes in Serratia spp. and Yersinia frederiksenii

Occurrence of sep Insecticidal Toxin Complex Genes in Serratia spp. and Yersinia frederiksenii

The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes

Behavior of Yersinia enterocolitica in the Presence of the Bacterivorous Acanthamoeba castellanii

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