Virulence Characteristics and Antibiotic Resistance Patterns among Various Phylogenetic Groups of Uropathogenic <i>Escherichia coli</i> Isolates

Abstract

SUMMARY:The aim of this study was to determine the resistance patterns of uropathogenic Escherichia coli (UPEC) isolates and to investigate the frequency of several virulence genes, including fimH, papA, hlyD, cnf-1, sitA, and tsh, among various phylogenetic groups of UPEC isolates. A total of 85 E. coli isolates were recovered from urine samples from outpatients with a clinical diagnosis of uncomplicated urinary tract infections. A molecular approach to examine the antimicrobial resistance patterns was employed using PCR and the disc diffusion method. The detected frequencies of the virulence factor genes determined using PCR were: fimH (34.1z), papA (9.4z), hlyD (21.2z), cnf-1 (3.5z), sitA (15.3z), and tsh (27.1z). These results revealed that the isolates were resistant to trimethoprimsulfamethoxazole (SXT) (74.1z), cefotaxime (CTX) (68.2z), and amoxicillin-clavulanic acid (AMC) (94.1z), and they were relatively less resistant to N (56.5z). According to these results, further investigation is needed to determine exactly whether or not SXT, CTX, and AMC are appropriate antibiotics for the treatment of UPEC infections in southern Iran. Although these results demonstrate that fimH is the most frequent virulence gene among UPEC isolates, the high prevalence of isolates that do not encode fimH (75.9z) and the relatively low frequency of isolates that carry other virulence genes require further investigation to clarify the role of the other potential virulence factors in the pathogenesis of these isolates.

Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity

“…These strains were mainly associated with phylogenetic groups, B2 and D as described other studies; however, they also have been associated with phylogenetic group A (Ejrnæs et al, 2011; Zhao et al, 2015). These UPEC strains could be considered community strains with predisposing factors that facilitate UTIs in immunocompromised patients, as previously reported (Derakhshandeh et al, 2015; Rodrigues et al, 2015). UPEC strains of Mexican origin are mainly associated with phylogenetic group B2 (López-Banda et al, 2014).…”

“…Phylogenetic groups B2 and D are commonly related to UPEC strains collected from patients with UTIs; however, at a lower frequency, these strains are also related to phylogenetic groups A and B1. In addition, multidrug-resistance (MDR) and virulence factors described in UPEC strains are associated with phylogenetic group B2 (Derakhshandeh et al, 2015; Ferjani et al, 2015). Recently, the MDR-phenotype was found to be related to phylogenetic groups A and D; although, UPEC strains susceptible to most antibiotics are also related to phylogenetic group B2 (Ejrnæs et al, 2011).…”

Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

“…Similar study conducted by Kot et al [42], showed that 38.1% E. coli strains belonged to phylogenetic group B2, 35.3% to group D, 18.5% to group A, and 8.1% to group B1. Phylogenetic group A, represented 18% of isolates, which was higher than in studies conducted in South Korea 3.44% [39] and Iran 0.7% [47], but some studies found phylogroup A was the dominant phylogroup [9,15,48] suggesting that the colon may be the main reservoir for strains that cause urinary tract infections [9]. In some studies, phylogroup D was the dominant strain [25,49].…”

“…The genetic determinants that are studied includes those coding for type 1 [12,[14][15][16].Specific primers were used to amplify sequences of the fim, pap, sfa/foc, afa, hly, cnf, and aer operons. Details of primer sequences and predicted sizes of the amplified products are given in Table 1.…”

Produtos da Agrobiodiversidade: uma análise da qualidade dos sabonetes líquidos de plantas medicinais produzidos por agricultores familiares da Zona Oeste do Rio de Janeiro

“…Em uma feira popular do município de Currais Novos -RN foi realizada uma avaliação da qualidade de plantas medicinais adquiridas (31) O patógeno E.coli é um bacilo Gram negativo, causador de 80 a 90% das infecções no trato urinário; é um microrganismo comensal da microbiota intestinal (32) .…”