Virulence-associated gene pattern of porcine and human Yersinia enterocolitica biotype 4 isolates

Schneeberger, M.; Brodard, Isabelle; Overesch, Gudrun

doi:10.1016/j.ijfoodmicro.2014.12.029

Cited by 15 publications

(5 citation statements)

References 36 publications

(41 reference statements)

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“…Unlike our data, resistance to sulfamethoxazole and streptomycin of Y. enterocolitica isolated from pigs and humans is continuously reported [ 10 , 17 , 20 ]. In line with our findings, resistance to chloramphenicol has not been frequently found [ 35 ], which contradicts other reports on the high rate of Y. enterocolitica resistance to chloramphenicol among slaughtered pigs in Northern Italy and Croatia [ 17 , 20 , 36 ], and among Y. enterocolitica clinical isolates that appeared during the time of the Swedish outbreaks [ 37 ]. There is some controversy regarding the resistance to tetracycline as well.…”

Section: Discussionsupporting

confidence: 66%

Prevalence, Genetic Homogeneity, and Antibiotic Resistance of Pathogenic Yersinia enterocolitica Strains Isolated from Slaughtered Pigs in Bulgaria

et al. 2023

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Yersiniosis is the third most commonly reported foodborne zoonosis in the European Union. Here, we evaluated the prevalence of pathogenic Yersinia enterocolitica among healthy pigs (as a major reservoir) in a slaughterhouse in Bulgaria. A total of 790 tonsils and feces from 601 pigs were examined. Isolation and pathogenicity characterization was carried out by the ISO 10273:2003 protocol and Polymerase Chain Reaction (PCR), detecting the 16S rRNA gene, attachment and invasion locus (ail), Yersinia heat-stable enterotoxin (ystA), and Yersinia adhesion (yadA) genes. Genetic diversity was assessed by pulsed-field gel electrophoresis (PFGE), and antimicrobial resistance by the standard disk diffusion method. Of all the pigs tested, 6.7% were positive for Y. enterocolitica. All isolates belonged to Y. enterocolitica bioserotype 4/O:3. ail, and ystA genes were detected in all positive strains (n = 43), while the plasmid Yersinia virulence plasmid (pYV) was detected in 41. High homogeneity was observed among the strains, with all strains susceptible to ceftriaxone, amikacin and ciprofloxacin, and resistant to ampicillin. In conclusion, a low prevalence of Y. enterocolitica 4/O:3 was found in healthy pigs slaughtered in Bulgaria, not underestimating possible contamination of pork as a potential risk to consumer health.

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Section: Discussionsupporting

confidence: 66%

Prevalence, Genetic Homogeneity, and Antibiotic Resistance of Pathogenic Yersinia enterocolitica Strains Isolated from Slaughtered Pigs in Bulgaria

et al. 2023

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“…In Brazil, Rusak et al () demonstrated resistance to ampicillin and cephalothin in Y. enterocolitica isolates of diverse origin. In Switzerland, Schneeberger, Brodard, and Overesch () observed resistance to ampicillin in B4 isolates from human clinical cases; only two isolates were resistant to chloramphenicol and nalidixic acid, and, additionally, one of these ones was resistant to sulfamethoxazole. In China, Ye et al () found that B1A O:8 isolates from retail foods were susceptible to kanamycin and sulfonamide, but resistant to trimethoprim/sulfamethoxazole (79.3%), ampicillin (91.4%), or cephalothin (91.4%).…”

Section: Discussionmentioning

confidence: 99%

Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile

Mastrodonato

Favier

Estrada

et al. 2018

Journal of Food Safety

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Yersinia enterocolitica is associated with human clinical manifestations. In this study, the bioserotype distribution, virulence potential, the antimicrobial susceptibility, and the genomic diversity of Y. enterocolitica isolates recovered from Argentina and Chile were assessed. Eight Chilean and 22 Argentine isolates recovered from human (7) and animal feces (3), foods (18) and wastewater (2) were analyzed. They belonged to the bioserotypes B2 O:9 (9), B1A O:5 (8), B1A O:41,42‐41,43 (8), B4 O:3 (3) and B1A O:7,8‐8‐8,19 (2). Autoagglutination (AA), Ca2+‐dependent growth at 37°C and yadA, inv and yst genes were observed in B2 O:9 and B4 O:3 strains. B1A strains exhibited the genotype yadA‐ inv‐ yst+. All strains were resistant to ampicillin, rifampicin and erythromycin. By XbaI‐PFGE, 11 genomic types (GTs) were demonstrated (D.I. 0.90). The characteristics of these Y. enterocolitica isolates highlight the importance to maintain active surveillance of this enteropathogen in both countries. Practical applications The trade exchange among Chile and Argentina includes 12–19% of agricultural goods and foodstuffs with fresh and processed vegetables, seafoods and meat products as the major commodities. Additionally, travel and tourism have increased in recent years with more than 2.5 million passengers moving from Argentina to Chile in 2017. In this context, the control of foodborne pathogens and diseases represents a challenge for both countries. The analysis of bioserotypes, virulence potential, antimicrobial susceptibility, and clonal relatedness of Argentine and Chilean Y. enterocolitica isolates encourages further studies to contribute to the knowledge of the epidemiology of this enteropathogen and the implementation of adequate sanitary measures to prevent Y. enterocolitica illness in both countries.

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“…However Fondrevez [ 15 ] modified this protocol and proved that streaking on CIN Agar from ITC broth could increase the number of positive samples in comparison with ISO 10273–2003 method [ 15 ]. The ITC/CIN culturing both favors the growth of biotype 4 [ 16 , 17 ] which is the most common biotype in Europe within humans and also animals such as slaughtered pigs [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Yersinia enterocolitica isolates from humans, pigs and wild boars by MALDI TOF MS

et al. 2018

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BackgroundYersinia enterocolitica is widespread within the humans, pigs and wild boars. The low isolation rate of Y. enterocolitica from food or environmental and clinical samples may be caused by limited sensitivity of culture methods. The main goal of present study was identification of presumptive Y. enterocolitica isolates using MALDI TOF MS. The identification of isolates may be difficult due to variability of bacterial strains in terms of biochemical characteristics. This work emphasizes the necessity of use of multiple methods for zoonotic Y. enterocolitica identification.ResultsIdentification of Y. enterocolitica isolates was based on MALDI TOF MS, and verified by VITEK® 2 Compact and PCR. There were no discrepancies in identification of all human’ and pig’ isolates using MALDI TOF MS and VITEK® 2 Compact. However three isolates from wild boars were not decisively confirmed as Y. enterocolitica. MALDI TOF MS has identified the wild boar’ isolates designated as 3dz, 4dz, 8dz as Y. enterocolitica with a high score of matching with the reference spectra of MALDI Biotyper. In turn, VITEK® 2 Compact identified 3dz and 8dz as Y. kristensenii, and isolate 4dz as Y. enterocolitica. The PCR for Y. enterocolitica 16S rDNA for these three isolates was negative, but the 16S rDNA sequence analysis identified these isolates as Y. kristensenii (3dz, 4dz) and Y. pekkanenii (8dz). The wild boar’ isolates 3dz, 4dz and 8dz could not be classified using biotyping. The main bioserotype present within pigs and human faeces was 4/O:3. It has been shown that Y. enterocolitica 1B/O:8 can be isolated from human faeces using ITC/CIN culturing.ConclusionThe results of our study indicate wild boars as a reservoir of new and atypical strains of Yersinia, for which protein and biochemical profiles are not included in the MALDI Biotyper or VITEK® 2 Compact databases. Pigs in the south-west Poland are the reservoir for pathogenic Y. enterocolitica strains. Four biochemical features included in VITEK® 2 Compact known to be common with Wauters scheme were shown to produce incompatible results, thus VITEK® 2 Compact cannot be applied in biotyping of Y. enterocolitica.

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Virulence-associated gene pattern of porcine and human Yersinia enterocolitica biotype 4 isolates

Cited by 15 publications

References 36 publications

Prevalence, Genetic Homogeneity, and Antibiotic Resistance of Pathogenic Yersinia enterocolitica Strains Isolated from Slaughtered Pigs in Bulgaria

Prevalence, Genetic Homogeneity, and Antibiotic Resistance of Pathogenic Yersinia enterocolitica Strains Isolated from Slaughtered Pigs in Bulgaria

Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile

Identification of Yersinia enterocolitica isolates from humans, pigs and wild boars by MALDI TOF MS

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