Virulence and molecular polymorphism of Prunus necrotic ringspot virus isolates.

Hammond, Rosemarie W.; Crosslin, J. M.

doi:10.1099/0022-1317-79-7-1815

Cited by 36 publications

(31 citation statements)

References 30 publications

(50 reference statements)

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“…Multiple sequence alignment revealed its high conservation in N-terminal except few changes, while the C-terminal was variable. The MP showed similarity to the level of 97-86 % at nucleotide and 98-92 % at amino acid level with MP from other isolates (data not shown) which is in accordance to previous reports [16]. The MP of Indian isolate was found to be highly similar (98 %) to PNRSV-apple isolate (accession no.…”

Section: Rnasupporting

confidence: 79%

“…The presence of methionine (M) at position 90 of PNRSV CP sequence was recognized here as a specific feature of rose isolate of PNRSV. All group I isolates of PNRSV belongs to the rugose pathotype, similar substitutions as found in MP of Indian isolate of PNRSV were also recorded previously [16,40]. Group II isolates have been previously characterized as mild pathotypes [16,40].…”

Section: Discussionmentioning

confidence: 58%

“…All group I isolates of PNRSV belongs to the rugose pathotype, similar substitutions as found in MP of Indian isolate of PNRSV were also recorded previously [16,40]. Group II isolates have been previously characterized as mild pathotypes [16,40]. Group III isolates were closest to an ApMV isolate (NC_003480), which was used as an outgroup in the phylogenetic analysis [40].…”

Section: Discussionmentioning

confidence: 93%

“…Presence of two amino acid insertions (NRNR) at position 39 in PNRSV CP has also been reported previously and was considered as characteristic feature of PV 32 group (Group I) of PNRSV isolates [3,16,17,26,34,40]. The significance of this insertion to biological activity is not known, but it does serve as a group specific feature of the group I isolates that can be used to discriminate between isolates in group I and III.…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization and intermolecular interaction of coat protein of Prunus necrotic ringspot virus: implications for virus assembly

Kulshrestha¹,

Hallan²,

Sharma

et al. 2013

Indian J. Virol.

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Coat protein (CP) and RNA3 from Prunus necrotic ringspot virus (PNRSV-rose), the most prevalent virus infecting rose in India, were characterized and regions in the coat protein important for self-interaction, during dimer formation were identified. The sequence analysis of CP and partial RNA 3 revealed that the rose isolate of PNRSV in India belongs to PV-32 group of PNRSV isolates. Apart from the already established group specific features of PV-32 group member's additional group-specific and host specific features were also identified. Presence of methionine at position 90 in the amino acid sequence alignment of PNRSV CP gene (belonging to PV-32 group) was identified as the specific conserved feature for the rose isolates of PNRSV. As protein-protein interaction plays a vital role in the infection process, an attempt was made to identify the portions of PNRSV CP responsible for self-interaction using yeast two-hybrid system. It was found (after analysis of the deletion clones) that the C-terminal region of PNRSV CP (amino acids 153-226) plays a vital role in this interaction during dimer formation. N-terminal of PNRSV CP is previously known to be involved in CP-RNA interactions, but our results also suggested that N-terminal of PNRSV CP represented by amino acids 1-77 also interacts with C-terminal (amino acids 153-226) in yeast two-hybrid system, suggesting its probable involvement in the CP-CP interaction.

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Section: Rnasupporting

confidence: 79%

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular characterization and intermolecular interaction of coat protein of Prunus necrotic ringspot virus: implications for virus assembly

Kulshrestha¹,

Hallan²,

Sharma

et al. 2013

Indian J. Virol.

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“…There are different opinions on the correlation between the amino acid sequences in MP and/or CP of Prunus isolates of PNRSV and the host specificity, symptoms or serological properties of the virus (Hammond and Crosslin 1998;Aparicio et al 1999;Hammond 2003;Vašková et al 2000;Glasa et al 2002;Spiegel et al 2004). Only Moury et al (2001) studied and discussed this subject for rose isolates of the virus.…”

Section: Introductionmentioning

confidence: 99%

Biological and molecular characterization of Prunus necrotic ringspot virus isolates from three rose cultivars

Paduch-Cichal

Sala-Rejczak

2011

Acta Physiol Plant

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Prunus necrotic ringspot virus (PNRSV) is a rose and stone fruit tree pathogen. Three different PNRSV isolates, originating from three rose cultivars were studied. These PNRSV isolates were characterized using molecular techniques. Nearly the complete nucleotide sequence (1,630 nucleotides) of RNA3 of the isolate PNRSV-R1 has been determined (GenBank Acc. No. DQ003584). The sequence of the MP gene of the PNRSV-R1 isolate was determined, the first such results for a rose-derived PNRSV isolate. The reaction of PNRSV infection on test plants was also investigated. Cucumis sativus cv. Wisconsin, Cucurbita maxima cv. Buttercup and Cucurbita pepo cv. Melonowa _ Zółta appeared to be the most useful test plants for the differentiation of isolate-specific pathogenicity.

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Molecular Evolution of the Plant Virus Family Bromoviridae Based on RNA3-Encoded Proteins

et al. 2005

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We have carried out an evolutionary study of the two proteins encoded by the RNA 3 from members of the plant virus family Bromoviridae. Using maximum likelihood methods, we have inferred the patterns of amino acid substitution that better explain the diversification of this viral family. The results indicate that the molecular evolution of this family was rather complex, with each protein evolving at different rates and according to different patterns of amino acid substitution. These differences include different amino acid equilibrium frequencies, heterogeneity in substitution rates among sites, and covariation among sites. Despite these differences, the model of protein evolution that better fits both proteins is one specifically proposed for the evolution of globular proteins. We also found evidence for coevolution between domains of these two proteins. Finally, our analyses suggest that the molecular clock hypothesis does not hold, since different lineages evolved at different rates. The implications of these results for the taxonomy of this important family of plant viruses are discussed.

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Virulence and molecular polymorphism of Prunus necrotic ringspot virus isolates.

Cited by 36 publications

References 30 publications

Molecular characterization and intermolecular interaction of coat protein of Prunus necrotic ringspot virus: implications for virus assembly

Molecular characterization and intermolecular interaction of coat protein of Prunus necrotic ringspot virus: implications for virus assembly

Biological and molecular characterization of Prunus necrotic ringspot virus isolates from three rose cultivars

Molecular Evolution of the Plant Virus Family Bromoviridae Based on RNA3-Encoded Proteins

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