Virulence and molecular genetic diversity of the Puccinia triticina population in Hebei Province of China in 2008 and 2010

Zhang, L. Y.; Meng, Qingfang; Yan, Huihuang; Liu, D. Q.

doi:10.1007/s10658-018-1549-x

Cited by 5 publications

(10 citation statements)

References 32 publications

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“…In one of our earlier studies, a wheat line introgressed with the Lr47 gene exerted high resistance to the majority of leaf rust races collected in China, including FHPR, THJS, THTS, FHJQ, PHSL, PHJT, PHSS, KHSS, PHTT, THTT, FHTR, FHHT, TGGT, FHTT, and FGMT [23]. The leaf rust race THTT was predominant in China and showed high virulence to most of the designated Lr genes [5]. In the current investigation, we collected wheat lines introgressed with the Lr47 gene in different spring common wheat cultivars “Yecora Rojo”, “UC1037”, and “White Yecora” from a previous research [23].…”

Section: Resultsmentioning

confidence: 99%

“…In China, due to the climate change and the large-scale cultivation of less diversified wheat cultivars, the epidemic area of wheat leaf rust has expanded from the Yellow-Huai River Valley area to the major wheat-growing regions [3,4]. In the year of 2012, an epidemic of wheat leaf rust occurred in China on more than 15 million ha and led to an approximately 3 million ton yield losses [5,6]. Currently, the genetic improvement of wheat cultivars using different Lr genes is still the most effective solution for this issue.…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Expression Profiling of Genes Associated with the Lr47-Mediated Wheat Resistance to Leaf Rust (Puccinia triticina)

Gao

et al. 2019

IJMS

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Puccinia triticina (Pt), the causal agent of wheat leaf rust, is one of the most destructive fungal pathogens threatening global wheat cultivations. The rational utilization of leaf rust resistance (Lr) genes is still the most efficient method for the control of such diseases. The Lr47 gene introgressed from chromosome 7S of Aegilops speltoides still showed high resistance to the majority of Pt races collected in China. However, the Lr47 gene has not been cloned yet, and the regulatory network of the Lr47-mediated resistance has not been explored. In the present investigation, transcriptome analysis was applied on RNA samples from three different wheat lines (“Yecora Rojo”, “UC1037”, and “White Yecora”) carrying the Lr47 gene three days post-inoculation with the epidemic Pt race THTT. A comparison between Pt-inoculated and water-inoculated “Lr47-Yecora Rojo” lines revealed a total number of 863 upregulated (q-value < 0.05 and log2foldchange > 1) and 418 downregulated (q-value < 0.05 and log2foldchange < −1) genes. Specifically, differentially expressed genes (DEGs) located on chromosomes 7AS, 7BS, and 7DS were identified, ten of which encoded receptor-like kinases (RLKs). The expression patterns of these RLK genes were further determined by a time-scale qRT-PCR assay. Moreover, heatmaps for the expression profiles of pathogenesis-related (PR) genes and several transcription factor gene families were generated. Using a transcriptomic approach, we initially profiled the transcriptional changes associated with the Lr47-mediated resistance. The identified DEGs, particularly those genes encoding RLKs, might serve as valuable genetic resources for the improvement of wheat resistance to Pt.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Expression Profiling of Genes Associated with the Lr47-Mediated Wheat Resistance to Leaf Rust (Puccinia triticina)

Gao

et al. 2019

IJMS

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“…The genomic DNA of each isolate sample was extracted from the urediniospores according to the modified Cetyltrimethylammonium Bromide (CTAB) method described by Gao (2003). The 21 pairs of EST-SSR primers (Supplementary Table S2) developed by Wang et al (2010aWang et al ( , 2010b were used for PCR reaction, and the total reaction system and amplification procedure were performed as described by Zhang et al (2018). PCR products were separated by 10% (w/v) polyacrylamide gel in 0.5× TBE buffer (45 mM Tris, 45 mM boric acid, and 1 mM EDTA), visualized by silver staining.…”

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

“…However, it only revealed the population differences between 2 years in Hebei Province, which did not fully reveal the P. triticina population dynamic patterns in this region. In this study, we analyzed the population genetic diversity of P. triticina in Hebei Province for 10 years (2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010) based on the results of Zhang et al (2018), which can provide a basis for further exploring the race variation patterns of P. triticina and the molecular mechanism of race virulence evolution.…”

Section: Introductionmentioning

confidence: 99%

Virulence and molecular genetic diversity, variation, and evolution of the Puccinia triticina population in Hebei Province of China from 2001 to 2010

Zhang

Zhang²,

Meng³

et al. 2023

Front. Plant Sci.

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Wheat leaf rust, caused by Puccinia triticina, is one of the most important fungal diseases of wheat in China. However, little is known about the dynamic changes of population structure and genetic diversity of P. triticina during a period of time. In this study, 247 isolates of P. triticina collected from Hebei Province from 2001 to 2010 were tested on 36 Thatcher near-isogenic lines for virulence diversity and detected by 21 pairs of Expressed Sequence Tag derived Simple Sequence Repeat (EST-SSR) primers for genetic diversity. A total of 204 isolates were successfully identified as 164 races, and THTT, THST, PHRT, THTS, and PHTT were the most common races in Hebei Province from 2001 to 2010. The cluster analysis based on virulence showed that P. triticina has a rich virulence polymorphism, which had a certain correlation with the years, while the cluster analysis based on EST-SSR showed that the genetic diversity of the P. triticina population was significantly different between years in Hebei Province from 2001 to 2010. In addition, the population structure of P. triticina may have changed greatly in 2007 and 2009, which was significantly different from that of 2001–2006 on either virulence or genetic characteristics. The variation frequency of the population structure had an increasing trend during this period. From 2001 to 2010, there was a certain degree of gene flow among the P. triticina populations. No significant correlation was found between virulence and molecular polymorphism. The genetic differentiation analysis of the 10 tested populations (each year as a population) showed that the coefficient of genetic differentiation (Gst) was 0.27, indicating that there was a certain genetic differentiation among or within populations of P. triticina in Hebei Province. The genetic variation within populations (73.08%) was higher than that among populations (26.92%), which indicated that the genetic variations were mainly found within populations. Our study provides the foundation for a better understanding of the population structure change and genetic diversity of P. triticina over a period in Hebei Province of China.

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“…( Pt ) is a widespread fungal disease that often leads to significant wheat yield losses ( Huerta-Espino et al, 2011 ; Singh et al, 2016 ). China is the world’s largest wheat producer, and leaf rust epidemics affect approximately 15 million ha in China annually ( Liu and Chen, 2012 ; Zhang L. et al, 2019 ). Breeding and planting wheat cultivars resistant to leaf rust can reduce fungicide use and is thus an economical and environmentally friendly strategy for managing this disease.…”

Section: Introductionmentioning

confidence: 99%

Identification and Validation of a Major Quantitative Trait Locus for Adult Plant Resistance Against Leaf Rust From the Chinese Wheat Landrace Bai Qimai

et al. 2022

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Leaf rust caused by Puccinia triticina Eriks. (Pt) is a common disease of wheat worldwide. The Chinese wheat landrace Bai Qimai (BQM) has shown high resistance to leaf rust for a prolonged period of time; the infected leaves of BQM displayed high infection types (ITs), but they showed low disease severities at the adult plant stage. To find quantitative trait loci (QTL) for resistance to leaf rust, 186 recombinant inbred lines from the cross Nugaines × BQM were phenotyped for leaf rust response in multiple field environments under natural Pt infections and genotyped using the 90K wheat single nucleotide polymorphism (SNP) chip and simple sequence repeat (SSR) markers. A total of 2,397 polymorphic markers were used for QTL mapping, and a novel major QTL (QLr.cau-6DL) was detected on chromosome 6DL from BQM. The effectiveness of QLr.cau-6DL was validated using the three additional wheat populations (RL6058 × BQM, Aikang58 × BQM, and Jimai22 × BQM). QLr.cau-6DL could significantly reduce leaf rust severities across all tested environments and different genetic backgrounds, and its resistance was more effective than that of Lr34. Moreover, QLr.cau-6DL acted synergistically with Lr34 to confer strong resistance to leaf rust. We believe that QLr.cau-6DL should have high potential value in the breeding of wheat cultivars with leaf rust resistance.

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Virulence and molecular genetic diversity of the Puccinia triticina population in Hebei Province of China in 2008 and 2010

Cited by 5 publications

References 32 publications

Genome-Wide Expression Profiling of Genes Associated with the Lr47-Mediated Wheat Resistance to Leaf Rust (Puccinia triticina)

Genome-Wide Expression Profiling of Genes Associated with the Lr47-Mediated Wheat Resistance to Leaf Rust (Puccinia triticina)

Virulence and molecular genetic diversity, variation, and evolution of the Puccinia triticina population in Hebei Province of China from 2001 to 2010

Identification and Validation of a Major Quantitative Trait Locus for Adult Plant Resistance Against Leaf Rust From the Chinese Wheat Landrace Bai Qimai

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