Virulence and genotype-associated infectivity of interferon-treated macrophages by porcine reproductive and respiratory syndrome viruses

García-Nicolás, Obdulio; Baumann, Arnaud; Vielle, Nathalie J.; Gómez-Laguna, J.; Quereda, J.J.; Pallarés, F. J.; Ramis, G.; Carrasco, L.; Summerfield, Artur

doi:10.1016/j.virusres.2013.08.009

Cited by 37 publications

(41 citation statements)

References 34 publications

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“…M2 macrophages have been reported to be more susceptible than M1 macrophages to PRRSV infection and to induce higher levels of anti-inflammatory cytokines (e.g. IL-10), enhanced phagocytic activity but reduced antigen presenting functions (Mosser, 2003;García-Nicolás et al, 2014). In the present study, SU1-bel infected piglets showed the highest increase in the number of macrophages both in the cortex and in the medulla of the thymus.…”

Section: Discussionsupporting

confidence: 44%

Thymic depletion of lymphocytes is associated with the virulence of PRRSV-1 strains

Amarilla¹,

Gómez-Laguna²,

Carrasco³

et al. 2016

Veterinary Microbiology

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Section: Discussionsupporting

confidence: 44%

Thymic depletion of lymphocytes is associated with the virulence of PRRSV-1 strains

Amarilla¹,

Gómez-Laguna²,

Carrasco³

et al. 2016

Veterinary Microbiology

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“…In most studies, monocytes were cultivated in the presence of M-CSF/GM-CSF [11] or using LM-929 (murine fibroblast cells expressing murine M-CSF and GM-CSF) cell supernatant [9, 10, 41] to prepare MDMs. In the present study, MDMs were prepared without the addition of cytokines, similarly to Vicenova et al [31] and García-Nicolás et al [12]. The aim of the presented study was to compare the susceptibility of two different types of macrophages to the infection with PRRS virus and to co-infection with H. parasuis.…”

Section: Discussionmentioning

confidence: 99%

“…While alveolar macrophages were relatively resistant to cytopathogenic effect caused by PRRSV, monocyte derived macrophages were much more sensitive to PRRSV infection (Figures 2 and 3). A recent study by García-Nicolás et al [12] showed decreased resistance to PRRSV infection of MDMs non-stimulated by cytokines. In contrast, IFN-γ or IFN-β pre-treated MDMs were protected against cytopathogenic effect caused by different strains of the PRRS virus (including strain Lelystad LVP23) [12].…”

Section: Discussionmentioning

confidence: 99%

“…Freshly isolated peripheral blood monocytes express extremely low levels of CD163 and are not susceptible to PRRSV infection [5] but as monocytes differentiate/age, the expression of CD163 increases and their susceptibility to PRRSV infection is enhanced [9]. Monocyte-derived macrophages (MDMs) [10] can be used as an alternative model for in vitro infection with PRRSV [5, 11, 12]. …”

Section: Introductionmentioning

confidence: 99%

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Concurrent infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis in two types of porcine macrophages: apoptosis, production of ROS and formation of multinucleated giant cells

Kavanová

Matiašková

Levá

et al. 2017

Vet Res

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Porcine reproductive and respiratory syndrome (PRRS) is one of the most significant and economically important infectious diseases affecting swine worldwide and can predispose pigs to secondary bacterial infections caused by, e.g. Haemophilus parasuis. The aim of the presented study was to compare susceptibility of two different types of macrophages which could be in contact with both pathogens during infection with PRRS virus (PRRSV) and in co-infection with H. parasuis. Alveolar macrophages (PAMs) as resident cells provide one of the first lines of defence against microbes invading lung tissue. On the other hand, monocyte derived macrophages (MDMs) represent inflammatory cells accumulating at the site of inflammation. While PAMs were relatively resistant to cytopathogenic effect caused by PRRSV, MDMs were much more sensitive to PRRSV infection. MDMs infected with PRRSV increased expression of pro-apoptotic Bad, Bax and p53 mRNA. Increased mortality of MDMs may be also related to a higher intensity of ROS production after infection with PRRSV. In addition, MDMs (but not PAMs) infected with H. parasuis alone formed multinucleated giant cells (MGC); these cells were not observed in MDMs infected with both pathogens. Higher sensitivity of MDMs to PRRSV infection, which is associated with limited MDMs survival and restriction of MGC formation, could contribute to the development of multifactorial respiratory disease of swine.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-017-0433-6) contains supplementary material, which is available to authorized users.

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“…Porcine monocyte-derived macrophages (MDM) were generated as previously described [49]. Briefly, peripheral blood mononuclear cells were isolated from the blood of 6- to 18-month-old specific pathogen-free (SPF) Large White pigs from the breeding facility of The Institute of Virology and Immunology IVI in Switzerland (in compliance with the Swiss animal protection law, under licence number BE88/14 approved by the animal welfare committee of the canton of Bern, Switzerland) using ficoll-paque density centrifugation (1.077 g/L; GE Healthcare Life Sciences).…”

Section: Methodsmentioning

confidence: 99%

Heterogeneous antigenic properties of the porcine reproductive and respiratory syndrome virus nucleocapsid

Rappe

García-Nicolás²,

Flückiger³

et al. 2016

Vet Res

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Porcine reproductive and respiratory syndrome virus (PRRSV) is an arterivirus responsible for a widespread contagious disease of domestic pigs with high economic impact. Switzerland is one of the rare PRRSV-free countries in Europe, although sporadic outbreaks have occurred in the past. The PRRSV isolate IVI-1173 from the short outbreak in Switzerland in 2012 was entirely sequenced, and a functional full-length cDNA clone was constructed. Genetic and antigenic characterization of IVI-1173 revealed the importance of amino acid 90 of the nucleocapsid protein N as part of a conformational epitope. IVI-1173 was not detected by SDOW17, a monoclonal antibody against N widely used to detect PRRSV-infected cells. Substitution of alanine at position 90 of N [N(A90)] with a threonine [N(T90)] restored reactivity of vIVI1173-N(T90) to SDOW17 completely. The relevance of this amino acid for the conformational SDOW17 epitope of PRRSV N was further confirmed by the opposite substitution in a functional cDNA clone of the genotype 2 isolate RVB-581. Finally, N proteins from ten genotype 1 strains differing from threonine at position 90 were analysed for reactivity with SDOW17. N(A90) totally disrupted or severely affected the epitope in 7 out of 8 strains tested. Based on these findings, 225 genotype 1 strains were screened for the prevalence of N(A90). N(A90) is rare in classical subtype 1 and in subtype 3 strains, but is frequent in Russian subtype 1 (70%) and in subtype 2 (45%) isolates. In conclusion, this study highlights the variable antigenic properties of N among genotype 1 PRRSV strains.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-016-0399-9) contains supplementary material, which is available to authorized users.

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Virulence and genotype-associated infectivity of interferon-treated macrophages by porcine reproductive and respiratory syndrome viruses

Cited by 37 publications

References 34 publications

Thymic depletion of lymphocytes is associated with the virulence of PRRSV-1 strains

Thymic depletion of lymphocytes is associated with the virulence of PRRSV-1 strains

Concurrent infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis in two types of porcine macrophages: apoptosis, production of ROS and formation of multinucleated giant cells

Heterogeneous antigenic properties of the porcine reproductive and respiratory syndrome virus nucleocapsid

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