Virulence and antibiotic-resistance genes in Enterococcus faecalis associated with streptococcosis disease in fish

Akter, Tasmina; Haque, Md. Najmul; Ehsan, Rakib; Paul, Sulav Indra; Foysal, Javed; Tay, Chin Yen; Islam, Md. Saiful; Rahman, Mohammad Mafizur

doi:10.1038/s41598-022-25968-8

Cited by 14 publications

(3 citation statements)

References 59 publications

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“…Fish vibriosis is widespread in the seas and brackish waters, affecting both marine and coastal freshwater fish of various species, including salmon, cod, eel, herring, perch, and flounder [71]. Along with fish vibriosis, Aeromonas septicemia, edwardsiellosis, columnaris and streptococcus, as well as a number of other diseases, are largely responsible for economic losses in aquaculture production [72][73][74][75][76][77][78][79][80][81][82][83][84].…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation and auto-aggregation abilities of novel targeted aqua-probiotics

Մանվելյան¹,

Բալայան²,

Miralimova³

et al. 2023

FFHD

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Background: The probiotics’ auto-aggregation and biofilm formation abilities have a significant role in the development of biotechnological processes.Objective: The aim of this study was to evaluate the biofilm formation and auto-aggregation abilities of novel, targeted aqua-probiotics isolated from aquatic organisms.Methods: The biofilm formation abilities of Lactobacillus delbrueckii str. UZ-1, Lactiplantibacillus plantarum str. R3, Lactococcus str. UZ-2, Enterococcus faecium str. R2, Pediococcus acidilactici str. N from the culture collection of the Microbiology of the Academy of Sciences of the Republic of Uzbekistan, Bacillus subtilis str. 1R, Bacillus amyloliquefaciens str. 4R and from the culture collection of the Southern Federal University of Russa and Lacticaseibacillus rhamnosus str. 1A and Enterococcus str. 9-3 from the culture collection of the Armenian National Agrarian University were assessed.Results: According to the investigations, the biofilm formation abilities of Lactobacillus delbrueckii str. UZ-1, Lactiplantibacillus plantarum str. R3, Lactococcus str. UZ-2, Enterococcus faecium str. R2, Pediococcus acidilactici str. N, Bacillus subtilis str. 1R, Bacillus amyloliquefaciens str. 4R, Bacillus amyloliquefaciens str. 5R, Lacticaseibacillus rhamnosus str. 1A and Enterococcus str. 9-3 were 0.119 ± 0.05D, 0.113 ± 0.065D, 0.196 ± 0.04D, 0.116 ± 0.01D, 0.152 ± 0.05D, 0.74 ± 0.15D, 2.621± 0.55D, 1.831 ± 0.45D, and 0.227 ± 0.04D and 0.483 ± 0.15D respectively. The highest rate of auto-aggregation was shown by Bacillus amyloliquefaciens str. 5R, and Bacillus amyloliquefaciens str. 4R was the strain with the highest ability to form biofilm. These two Bacillus strains are also distinguished by the highest DNA protective properties and relatively low antioxidant activity. Despite the fact that Bacillus amyloliquefaciens str. 5R showed the highest rate of auto-aggregation after 2 hours, this strain showed the lowest level of auto-aggregation among the studied strains after 24 hours. The Enterococcus str. 9-3 strain with the highest antioxidant activity showed 0.483 ± 0.15D biofilm formation ability.Conclusion: The novel targeted aquaprobiotics have distinct biofilm formation and aggregation properties, which are important to consider when planning appropriate biotechnological processes, requiring specific membrane properties of probiotics.Graphical Abstract: Membrane properties of novel targeted aquaprobiotics.Keywords: Lactobacilli, aqua-probiotic, antioxidant activity, biofilm formation, aggregation, Enterococcus str. 9-3

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Section: Discussionmentioning

confidence: 99%

Biofilm formation and auto-aggregation abilities of novel targeted aqua-probiotics

Մանվելյան¹,

Բալայան²,

Miralimova³

et al. 2023

FFHD

View full text Add to dashboard Cite

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“…Briefly, each set of query proteins was aligned with the databases, and annotations of the best-matched subjects ( e -value < 10 −5 ) were obtained for gene annotation. Based on the Comprehensive Antibiotic Resistance Database (CARD) and the Virulence Factor Database (VFDB), the antibiotic resistance and virulence genes for each Salmonella isolate were evaluated ( Akter et al, 2023 ). The Plasmid replicons for the genome sequences were studied using PlasmidFinder ( Akter et al, 2023 ).…”

Section: Methodsmentioning

confidence: 99%

“…Based on the Comprehensive Antibiotic Resistance Database (CARD) and the Virulence Factor Database (VFDB), the antibiotic resistance and virulence genes for each Salmonella isolate were evaluated ( Akter et al, 2023 ). The Plasmid replicons for the genome sequences were studied using PlasmidFinder ( Akter et al, 2023 ). The sequencing results for Salmonella strains were uploaded to the National Foodborne Disease Molecular Tracing Network (TraNet).…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial resistance and genomic investigation of Salmonella isolated from retail foods in Guizhou, China

Zhou,

Ye,

Zhou

et al. 2024

Front. Microbiol.

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IntroductionSalmonella is a major foodborne pathogen worldwide that causes severe morbidity and mortality. It is mainly caused by consuming contaminated food, with retail food considered the primary source.MethodsIn Guizhou, China, 102 Salmonella strains isolated from 2016 to 2021 underwent phenotypic antimicrobial resistance testing and whole-genome sequencing (WGS) to understand Salmonella diversity, including serotypes, sequencing types (STs), antimicrobial genes, virulence genes, plasmid types, multi-locus sequence types (MLST), and core genome MLST (cgMLST).Results and discussionS.Typhimurium was the dominant serotype, and O:4(B) was the leading serogroup. The most prevalent genotype was ST40. Phenotypic antimicrobial resistance identified 66.7% of the sampled isolates as multi-drug resistant (MDR). S.Enteritidis (n = 7), S.Typhimurium (n = 1), S.Indiana (n = 1), S.Kentucky (n = 1), S.Uganda (n = 1), all of which were MDR, were resistant to Colistin. Resistance rates varied significantly across different strains and food types, particularly meat products exhibiting higher resistance. Notably, significant increases in resistance were observed from 2016 to 2021 for the following: ≥ 1 resistant (P = 0.001), MDR (P = 0.001), ampicillin (P = 0.001), tetracycline (P < 0.001), chloramphenicol (P = 0.030), and trimethoprim/sulfamethoxazole (P = 0.003). The marked escalation in drug resistance over the recent years, coupled with the varying resistance rates among food sources, underscores the growing public health concern. Our findings highlight the need for a coordinated approach to effectively monitor and respond to Salmonella infections in Guizhou, China.

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Unravelling the genomic secrets of bacterial fish pathogens: a roadmap to aquaculture sustainability

Olymon,

Yadav,

Teronpi

et al. 2024

Mol Biol Rep

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Virulence and antibiotic-resistance genes in Enterococcus faecalis associated with streptococcosis disease in fish

Cited by 14 publications

References 59 publications

Biofilm formation and auto-aggregation abilities of novel targeted aqua-probiotics

Biofilm formation and auto-aggregation abilities of novel targeted aqua-probiotics

Antimicrobial resistance and genomic investigation of Salmonella isolated from retail foods in Guizhou, China

Unravelling the genomic secrets of bacterial fish pathogens: a roadmap to aquaculture sustainability

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